Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,110,421	C→A	noncoding (56/76 nt)	pheV →	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,110,421	0	C	A	100.0%	74.5 / NA	22	noncoding (56/76 nt)	pheV	tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/12);  total (10/12)

CCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTG  >  NC_000913/3110212‑3110661                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ccTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGCCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGccc                                                                                                                                                                                                         >  2:280931/1‑251 (MQ=255)             gCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGt                                                                                                                                                                                              <  2:76859/250‑1 (MQ=255)                    tCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGAAGAGCAGGGGATTGAAAATACCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTCAGAACCCGCCCACAAGGCGGGTTTTTGCtt                                                                                                                                                                                      >  2:97912/1‑251 (MQ=255)                              ggACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                                                                                                                                                                              <  1:280931/249‑1 (MQ=255)                                       ggTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGGTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAAcc                                                                                                                                                                    <  2:241308/250‑1 (MQ=255)                                            gCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTTATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGATCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                                                                                                                                                               <  2:236345/250‑1 (MQ=255)                                                                    cAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGTCGAATCAGGTTTAATGCGCCGCGTGGCCCGGATAGCTCATGCGGTAGAGCAGGGGATTGAAAATCCCCGGGTCCTTGGTTAGATTCCGAGTCCTGGCACCACTAAGTCTTAAGAACCCGTCCACAAGGCGGGTTTTTGCTGGTTGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTg                                                                                                                                      <  1:171877/251‑1 (MQ=255)                                                                                 gATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGGCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTActct                                                                                                                         <  2:226560/251‑1 (MQ=255)                                                                                            cTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                                                                                                                                                                              >  1:240882/1‑187 (MQ=255)                                                                                            cTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                                                                                                                                                                              <  2:240882/187‑1 (MQ=255)                                                                                             tGCAGTCAATTGAAGAAATTTGATTGCCGAGACGCGGCGCATCAGGTTTAATGAGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAACTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCCCGTTAAc                                                                                                             >  2:146088/1‑251 (MQ=38)                                                                                                aGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACgag                                                                                                          <  1:324516/251‑1 (MQ=255)                                                                                                aGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACg                                                                                                            >  1:63781/1‑249 (MQ=255)                                                                                                              aTTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGATGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGCCAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGCGAGTAATTTTATTgg                                                                                            >  2:134258/1‑250 (MQ=255)                                                                                                                        cgagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAtctc                                                                                  >  2:117276/1‑251 (MQ=255)                                                                                                                                                      ccGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGc                                                    <  2:63781/251‑1 (MQ=255)                                                                                                                                                             ccGGATAGCTCAGTCGGTAGAGCAGGTGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGc                                              >  1:196345/1‑250 (MQ=255)                                                                                                                                                                   aGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTc                                                                                                                                                                 >  1:285368/1‑129 (MQ=255)                                                                                                                                                                   aGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTc                                                                                                                                                                 <  2:285368/129‑1 (MQ=255)                                                                                                                                                                                  gCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGTAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTtct                        <  1:146088/251‑1 (MQ=255)                                                                                                                                                                                         aTTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCagcagc                 <  1:234908/251‑1 (MQ=255)                                                                                                                                                                                                        gtCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTtg  >  1:147280/1‑251 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTG  >  NC_000913/3110212‑3110661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑