Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,858	T→G	K129N (AAA→AAC)	fbaA ←	fructose‑bisphosphate aldolase class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,858	0	T	G	100.0%	175.7 / NA	49	K129N (AAA→AAC)	fbaA	fructose‑bisphosphate aldolase class II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/27);  total (22/27)

CAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGC  >  NC_000913/3070635‑3071094                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             cAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGGCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCg                                                                                                                                                                                                                   <  2:161712/251‑1 (MQ=255)  aaCGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTACATGTGCCTGTTGTACACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGACGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGCCAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGGAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCgacg                                                                                                                                                                                                                   >  1:212561/1‑247 (MQ=255)                 ggTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGtt                                                                                                                                                                                                   <  1:118810/251‑1 (MQ=255)                              gAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTg                                                                                                                                                                                      >  1:263941/1‑251 (MQ=255)                               aaGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTgg                                                                                                                                                                                     <  1:71754/251‑1 (MQ=255)                                     ccATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATGCGTCAGACAGGTCTATCATGTGAGAATAGAACAGCGGCTTACCGGTAGCTTCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAgt                                                                                                                                                                                <  1:47980/250‑1 (MQ=255)                                      cATGTGGCTGTTGTCCACGTCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAgtgt                                                                                                                                                                              <  1:41297/251‑1 (MQ=255)                                        tgtgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTcgcg                                                                                                                                                                                           >  1:57820/1‑236 (MQ=255)                                        tgtgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTcgcg                                                                                                                                                                                           <  2:57820/236‑1 (MQ=255)                                                 ttgccccGCCGTCGTCTGCTCCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTGTGGACAGGCGCTCCAGGTATTTAGAGCTGATTTCGATGTTCTCTTGCAGAGAGTCTTCAGACAGGTCGATCAGGTGAGAAGAGAACTGCGGCGTACCGGTTGCTGCGGAGTGGTTTTCTCCCGCGTCCAACAGATCATGGATCCTCTGCATCAGGGGCTTCGCTCAGTGGTCAGTGTGCAGGATAAc                                                                                                                                                                    <  1:222590/244‑1 (MQ=255)                                                 tgtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAAcc                                                                                                                                                                   >  1:54223/1‑251 (MQ=255)                                                     cACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGAGGTCAGTGTGCAGGATCACCGGa                                                                                                                                                                >  2:69010/1‑250 (MQ=255)                                                                  tcACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCGCTGCAGCAGTTTCGTCGCGCAGTTGGCATTGTGCAGGATAACCGGAACACCATAATg                                                                                                                                                     <  2:179288/248‑1 (MQ=255)                                                                           gTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTg                                                                                                                                         <  1:241835/251‑1 (MQ=255)                                                                                    aGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTGCGAGGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTGCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACTCCATAATGTTCAGCCATCGGGTGAACGTg                                                                                                                                <  2:229846/251‑1 (MQ=255)                                                                                       tCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGAGTTCGATGTTCTCTTGCAGAGAGTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAAGGTTCAGCCATCTGGTGAACGTGAt                                                                                                                              <  2:212561/250‑1 (MQ=255)                                                                                         gATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATgcg                                                                                                                           <  1:171701/251‑1 (MQ=255)                                                                                         gATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATgcg                                                                                                                           >  1:6859/1‑251 (MQ=255)                                                                                           tttCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTAGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACAACATAATGTTCAGCCATCTGGTGAACGTGATgcgc                                                                                                                          >  1:364539/1‑250 (MQ=255)                                                                                               cAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCAcc                                                                                                                       >  1:182040/1‑249 (MQ=255)                                                                                                     cATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTAAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCag                                                                                                                     >  2:172661/1‑245 (MQ=255)                                                                                                      aTGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATcgc                                                                                                              >  2:50532/1‑251 (MQ=255)                                                                                                                         gcTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCAccc                                                                                           >  1:187447/1‑251 (MQ=255)                                                                                                                            ccAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATGCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCTCGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACCCCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCATGATAGCAGCACCCTg                                                                                         <  1:121841/250‑1 (MQ=255)                                                                                                                              aGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCgg                                                                                      >  1:318325/1‑251 (MQ=255)                                                                                                                                   tttAGAGAAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGt                                                                                 >  1:250879/1‑251 (MQ=255)                                                                                                                                     tAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTc                                                                                <  2:217835/250‑1 (MQ=255)                                                                                                                                      agagCAGATTTCGATGTTCTCTTGCCGAGATTCTTCCGACCGGTCGAACATGTGAGAAGACCACAGCGGCTCCCCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCAAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGCACCCCATAATGTTCAGACAt                                                                                                                                            >  2:177752/1‑189 (MQ=255)                                                                                                                                      agagCAGATCTCGATGTTCTCTTGCAGAGATTCGTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCGGCGAAGTTGTTTTCTCCCGTGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCGTCGCGCGGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCAt                                                                                                                                            <  1:177752/189‑1 (MQ=255)                                                                                                                                        ggcagagttcgattgtcgctggCAGAGATTCGTCATACAGGTCGATCATGTGAGAAGAGAACAGCGTCTTACCGGTATCTGCGAAGTGGTTTTCGCCCGCGTCCAACAGACCGTCGATTCACGGCAGCAGTTTCTTCTCGCAGTGGGCAGTGTGCAGGATACCCGGAGCACCATAATGTGCAGCCATCTGGTGAACGTGATGCGCAGCATATAGCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGAt                                                                            <  2:201350/230‑1 (MQ=255)                                                                                                                                                 cGATGTTCTCTTGCAGAGATGCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCt                                                                   <  2:54223/251‑1 (MQ=255)                                                                                                                                                   aTGTTCTCTTGCAGCGATTCTTCAGACAGGTCGCTCATGTGAGAAGAGACCAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCCGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATTTTCAGCCATCTGGCGCACGTGATGCGCCCCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCttt                                                                 >  2:156125/1‑251 (MQ=255)                                                                                                                                                      ttCTCTTGCAGAGATTCTTCAGAAAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGCCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGGGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTAcc                                                              >  1:309611/1‑251 (MQ=255)                                                                                                                                                            tGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACTCTGCGGAACGTCAGATTTCACGCCTTTACCAGc                                                           <  1:184893/248‑1 (MQ=255)                                                                                                                                                                   aTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGaa                                                 <  1:53252/251‑1 (MQ=255)                                                                                                                                                                      cttcAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGc                                               >  2:69744/1‑250 (MQ=255)                                                                                                                                                                        tcAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGCAGTGGTTTTCACCCGCGACCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTAAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGCACGTCAGATTTCAAGCCTTTACCAGCGATAAAGGAAGcacc                                            >  2:227920/1‑251 (MQ=255)                                                                                                                                                                          aGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGcaccac                                          <  1:49223/251‑1 (MQ=255)                                                                                                                                                                             cAGGTAGATCATGTGAGAAGAGAACCGCGGATTACCGGTAGCTGCGACGTGGTTTTCACCCGCGTACAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATACCCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCCCCCTGCGGAACGTCAGATTTCACGCCTTTCCCAGCGCTAAAGGAAGCACCACCGt                                       >  2:328336/1‑251 (MQ=255)                                                                                                                                                                               ggTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACTCCTTTACCAGCGATAAAGGAAGCACCACCGTTg                                     <  2:182040/251‑1 (MQ=255)                                                                                                                                                                                  cGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGgag                                  <  1:69744/251‑1 (MQ=255)                                                                                                                                                                                    aTCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGaa                                <  2:318325/251‑1 (MQ=255)                                                                                                                                                                                       aTGTGAGAAGAGAACAGCGTCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAATCACGACGGTTGGAGAACTg                             <  2:315493/251‑1 (MQ=255)                                                                                                                                                                                                     cAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGgcg               >  1:263360/1‑251 (MQ=255)                                                                                                                                                                                                        cGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCTTCCAACAGACCGTCGATCCACGGCAGCAGGTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCtt            <  2:237317/251‑1 (MQ=255)                                                                                                                                                                                                         ggCTTACCAGTAGCGGCGAAGTTGTTTTCCCCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTGCGCGCAGTGGTCAGTGTGCAGTATAACCTGAACACCATAATGTGCAGCCATCTGGTGAACGTGATGCTCACCAGAGATCGCGCCCGGTATAGGAGCTCCCGGCTGAACGTCAGATTTCACGCCTTTACCAGCTATAAAGGAAGCACCTCTGTTGGAGGAGTTAACGATAACCGGCTCttt           <  2:222277/251‑1 (MQ=255)                                                                                                                                                                                                            ttACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAAc        >  2:84814/1‑251 (MQ=255)                                                                                                                                                                                                                cGGTAGCTGCGAAGTGGTTTTCCCCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTa    <  1:362436/251‑1 (MQ=255)                                                                                                                                                                                                                  gTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTagc  >  2:69519/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             CAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTTTAGC  >  NC_000913/3070635‑3071094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑