Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	83.6 / NA	27	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/11);  total (16/11)

ACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑GT‑‑AGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTAC  >  NC_000913/1301749‑1302210                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                           aCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGGCAACCGCGACC‑‑GTGGAGTGGTGAAATCTACATGACGTATAAc                                                                                                                                                                                                                       <  2:144499/251‑1 (MQ=255)             ccAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATc                                                                                                                                                                                                           <  2:21023/251‑1 (MQ=255)                                aaTTCGCCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAACGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                                         >  2:316160/1‑250 (MQ=255)                                   gcGGCGAAAAATGGACCCAGCCTGGTACTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAATTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTGCCAGAAGCTGaac                                                                                                                                                                                      <  2:359088/249‑2 (MQ=255)                                            aaTGGACCCAGACTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACTGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATcc                                                                                                                                                                            >  2:46891/1‑251 (MQ=255)                                              tGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                                          >  2:353682/1‑251 (MQ=255)                                                          ggTAATATCGTCACCAACGGTGCCTATTCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCGTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATTACGTATAACAGCa                                                                                                                                                                                                                   <  2:353918/198‑1 (MQ=255)                                                          ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCa                                                                                                                                                                                                                   >  1:353918/1‑198 (MQ=255)                                                                atCGTCACCAACGGTGCATATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTTTATTAACCCGGTAACCTATTTGCCTATTGCTTCTTAAGTTACAGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAGTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCTCGTTGa                                                                                                                                                         <  2:295618/250‑1 (MQ=255)                                                                            ggTGCCAATACCTTCAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGAAGCCCGACCTACTGGACCAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTAACGATGTCAACCGCTCGCGTGT‑‑AGTGGTGAAATCGACATGACGTCTAACAGCATGCCGATCGACTTGTTCCAGAAGCTGAAAAACGAGAACCCGGCCGAAGTTCACGTTGATCCATACAtgtg                                                                                                                                             >  2:285740/1‑250 (MQ=255)                                                                                   ataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACt                                                                                                                                     >  2:213916/1‑251 (MQ=255)                                                                                           aaaGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGAAATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCACGGCCGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTagga                                                                                                                              >  2:13466/1‑247 (MQ=255)                                                                                                   ggTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaaca                                                                                                                      >  1:289353/1‑250 (MQ=255)                                                                                                             gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                           >  2:81388/1‑251 (MQ=255)                                                                                                             gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                           <  2:73904/251‑1 (MQ=255)                                                                                                                        tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                                                                 >  2:255200/1‑250 (MQ=255)                                                                                                                          ttGAACGCAGCCCGACCTACCGGAACAACGCGAAAACCGTTATTAACCATGTAACCTATTTGCTTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACa                                                                                                                                                                                                                      <  1:204280/131‑1 (MQ=255)                                                                                                                          ttGAAAGCAGCCCGAACTACTGGAACAACGCGAAAACCGTTATTAACCCGGAAACCTATTTGCCTATTGCTTCTGCAGTTCCCGATGTCCACAGCTACCGTGT‑‑AGTGGTGAAATCGAAATGACGTATaaaa                                                                                                                                                                                                                      >  2:204280/1‑129 (MQ=255)                                                                                                                                                gAACAACGCTAAACCCGTTATTAACCAGGTAACCTATTTGCCTATGGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAGTAAGAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGaa                                                                        <  2:149872/251‑1 (MQ=255)                                                                                                                                                          aaacccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGCAACCGCCATTCAACGATGTGCGTGTGCGAACCGCACTGAAACTAGGTATg                                                              >  2:177799/5‑251 (MQ=255)                                                                                                                                                           aaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTACCAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATgg                                                             <  1:296478/251‑1 (MQ=255)                                                                                                                                                              ccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                                          <  1:213916/251‑1 (MQ=255)                                                                                                                                                                gTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATGGACcgc                                                        >  1:341524/1‑251 (MQ=255)                                                                                                                                                                          aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTg                                              >  2:328796/1‑251 (MQ=255)                                                                                                                                                                               aCCTATTTGCCTATTTCTTCTGAATTTACCGATGTCACCCGCTCCCGTGT‑‑AGTTGTGAAAGCGACATGACGTATAACAGCATGCTGATCGAATTGTTCCAGAAGCTGAAAAAAGATACCCCTGACGAAGTTCAGGTGGATCCATCCCTGTGCACTTTCTATTACGAATTTAACAACCAGAATCCTCCATTCAACGATGTGCGTGTGCGTACCGCACTGATACTAGTTATGGACCGCGATATTAGTGTtaat                                         <  2:336583/251‑1 (MQ=255)                                                                                                                                                                                                        ttACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGAAGGGCAACATGc                >  1:290355/1‑251 (MQ=255)                                                                                                                                                                                                                      cgctaccgtgT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTac  >  1:181123/10‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                           ACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑GT‑‑AGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTAC  >  NC_000913/1301749‑1302210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑