| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,528 | T→C | Q8R (CAG→CGG) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,528 | 0 | T | C | 97.3% | 127.1 / ‑6.6 | 37 | Q8R (CAG→CGG) | araD | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (15/21); minor base G (0/1); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCA > NC_000913/66287‑66772 | ggggAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg > 1:185733‑M1/1‑246 (MQ=255) ggAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTAAGCGGTCATGACGATGTAAACGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg > 1:238031‑M1/1‑244 (MQ=255) gAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagac > 1:60971‑M1/1‑243 (MQ=255) aaTGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacc < 1:192646‑M1/251‑10 (MQ=255) gCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc < 2:185733‑M1/251‑13 (MQ=255) ggCGTGTCGGAGGAGGGCTTTTTCGGTCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATAGCGTCAGCGGGCATGACGCTGTAATCGACGCCGGAAGGTTTGAGCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCTTCGcccgatacccgcacgcgtcggcctgcaggcatggag < 2:315063‑M1/251‑37 (MQ=255) cGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTTTAATACCGGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagt < 1:127448‑M1/251‑44 (MQ=255) aggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGAGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagag > 1:42990‑M1/1‑202 (MQ=255) ttCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgat > 1:59475‑M1/1‑194 (MQ=255) gTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaa < 1:81836‑M1/251‑61 (MQ=255) accctcaacCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCTGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTGTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcatgtatggataaacagtagagagttgcgataaaaa < 2:308514‑M1/246‑62 (MQ=255) cctgcaaccACCTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGAGGTCAGGACGCTGTAAGCGACGCCGGAAGGTGTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagatccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagc < 2:270865‑M1/247‑64 (MQ=255) cTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg > 2:58563‑M1/1‑187 (MQ=255) aCCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcagg < 2:60971‑M1/251‑70 (MQ=255) ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtaaggtagg > 2:293806‑M1/1‑177 (MQ=255) aCCGGTTGCGATGGTAACCGCGACCATAGCGTCAGCGGGCATGACGCTGTAATCGACGCCGGAAGGTTTGATCGCAAAGCCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGGTTCTAATACCCGGCGcccgagacccgcacgcgtcgaccggcaggtatggagaaacagtatagagttgcgataaaaagcgtcagttaggagcc < 2:247033‑M1/251‑78 (MQ=255) gCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTGTGAGCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTGCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagatagttgcgataaaaagcgtcaggtagtatccgctaatcttat < 2:43898‑M1/250‑89 (MQ=255) atCGTCAGCGGTCATGTCGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatg < 2:164138‑M1/251‑105 (MQ=255) atCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagcgagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatg > 1:286885‑M1/1‑147 (MQ=255) gCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagc < 1:362482‑M1/251‑112 (MQ=255) ggTCAGGACGCTGTAATCGACGCCGGAAGGTTTGAGCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGTCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaa < 1:239972‑M1/251‑114 (MQ=255) cATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCGGACGTTGCCCCATGTGAGCGGGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagt < 2:272341‑M1/251‑117 (MQ=255) aaTCGACGCCGGCAGGTTTGATCACAAAGACGCCGCGCTCGCGCTCAAAGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagcaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtg > 2:213075‑M1/1‑124 (MQ=255) cGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctcatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgca > 2:184796‑M1/1‑121 (MQ=255) ccGGAAGGTTTGATCACAAAGACGCCGCTCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGATGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagaaccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaggcgtcaggtaggatccgctaatcttatggataaaactgctatggcatcgcaacgggtgacgccgtgcaaataat > 2:213896‑M1/1‑116 (MQ=255) gTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtg < 1:269635‑M1/251‑143 (MQ=255) aTCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggactt < 1:94767‑M1/251‑148 (MQ=255) cgcgCTCGCGATCAACGGCGCTGACGTTGCCACATGTGAGCGTGACCAGGTTGTGTTTTCGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagccccgcaagcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgatt > 1:18481‑M1/1‑91 (MQ=255) cgcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattataga < 2:96472‑M1/250‑166 (MQ=255) gcgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttac < 1:199244‑M1/251‑178 (MQ=255) cgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacg < 1:5506‑M1/251‑179 (MQ=255) gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgc > 2:315682‑M1/1‑72 (MQ=255) gTTGCCCAATGTGAGCGTGACCAGGTTGGGTTTTGGCAGCGCCAGGTTGGCTTATAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgagttttt > 1:86378‑M1/1‑66 (MQ=255) tGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgcgtttttgtcatggctttggtcccgctttgttacagaat < 1:108134‑M1/250‑218 (MQ=255) ccAGGTTGGCTGCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgcgtttttgtcatggctttggtcccgctttgttacagaatgcttttaat < 1:142156‑M1/251‑227 (MQ=255) tCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgtggacttttctgccgtgattatagacacttttgttacgcgtttttgtcatggctttggtcccgctttgttacagaatgcttttaataagcggggtt < 1:3322‑M1/250‑237 (MQ=255) aaTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggcgaaaaagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgccgccgtgcaaataatcaatgtggccttttctgccgtgattatagacacttttgttacgcgtttttgtcatggctttggtcccgctttgttacagaatgcttttaataagcggggttaccg > 1:344577‑M1/1‑11 (MQ=255) | GGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCA > NC_000913/66287‑66772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |