Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,655,795	IS2 (–) +5 bp	intergenic (‑122/‑9)	ynfA ← / → ynfB	putative transporter YnfA/DUF1283 domain‑containing protein YnfB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1467910 =	NA (NA)	19 (0.550)	19/482	0.6	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 1655799	0 (0.000)					intergenic (‑126/‑9)	ynfA/ynfB	putative transporter YnfA/DUF1283 domain‑containing protein YnfB
*	?	NC_000913	= 1469240	NA (NA)	25 (0.730)	25/478	0.4	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	1655795 =	0 (0.000)					intergenic (‑122/‑13)	ynfA/ynfB	putative transporter YnfA/DUF1283 domain‑containing protein YnfB

GAAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTT  >  NC_000913/1467662‑1468139                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      gaAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGGTAGTGGTGAGTCGGCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAAGGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTgg                                                                                                                                                                                                                                     <  1:163749/251‑1 (MQ=255)  aaaaaCGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCTGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGa                                                                                                                                                                                                                                    <  1:200097/251‑1 (MQ=255)    aaaCGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGAtt                                                                                                                                                                                                                                  <  1:346780/251‑1 (MQ=255)               cAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGCGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTatat                                                                                                                                                                                                                       >  1:204276/1‑251 (MQ=255)               cAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGCCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCAGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGACCCCTGGATTTGCCCCTatat                                                                                                                                                                                                                       >  1:26717/1‑251 (MQ=255)                                       tGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACtt                                                                                                                                                                                                 <  2:70081/249‑1 (MQ=255)                                                                     ttgtcgtcagtggcgcatcctcgatataTCTGCATGGCGCTCCGACAATCAGCGGACAATTCCCTCTGGGGTTTGCCGGCGTTATTCGGCTCCAGGATACACCTTTGCTGGAACTTGGCAGTGGCGCTACGCTCACCATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCCGCCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATCTTc                                                                                                                                                                 >  2:180979/25‑251 (MQ=25)                                                                               tggtgAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCt                                                                                                                                                                                                                                       <  1:225114/171‑1 (MQ=255)                                                                               tggtgAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                                                                                                                         >  2:42334/1‑249 (MQ=255)                                                                               tggtgAATCGTCGCTATATCTGAATGGAGATCCGACAATCAGCGGCGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGGGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCt                                                                                                                                                                                                                                       >  2:225114/1‑171 (MQ=255)                                                                                                          agaTACGACAATCAGCGGAGCATACCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAACTTGGCAGTGGGGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGACCCTATATTTCCAGACATCTGTTATCACTTAACCACTTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGc                                                                                                                            >  2:311928/1‑251 (MQ=37)                                                                                                               cgataaTCAGCGGAGAATGCCCTCTGGGGTTTGCCGGGGTTAGTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGTGACAAGATCCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTGACCCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATTCCATTc                                                                                                                       <  2:116637/247‑1 (MQ=25)                                                                                                                            agaAGTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATGTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCg                                                                                                          <  2:12416/251‑1 (MQ=255)                                                                                                                               aTTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCGCGAAc                                                                                                       <  1:243754/251‑1 (MQ=255)                                                                                                                                                            gggTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGCTATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTa                                                                           >  1:307766/1‑250 (MQ=255)                                                                                                                                                                     aTAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAAAAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTGCTTTGCTGCCGTTAACCCGTCTgg                                                                 >  2:269744/1‑251 (MQ=255)                                                                                                                                                                                      aTTGGCAGTGGCGCTACGCTAAAAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATCTTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGGGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGCACGCCTCTGCAAGGTTCTTTTCTGCCGTTAACCCGTCTGGTTTGGGCATGTTACTgt                                                >  2:284140/1‑250 (MQ=255)                                                                                                                                                                                             gTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGTTTCTTTGCGGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTc                                         <  1:148891/251‑1 (MQ=255)                                                                                                                                                                                                          aaCAATGCAGGATATTGACAGTTTTGAACATTATGGGACAAGAACCCGGGATTGGCCCATATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATGCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAAGGCTCGAACGCCTCTGCAGGGTTCTTGGCTGCCGTTAATCCGTCTGGTTTGGGCAAGATTCTGATGTAGTCGCGCTTTATCGtt                            <  2:26717/251‑1 (MQ=17)                                                                                                                                                                                                                     atatTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTAGAATGCTCGAACGCCTCTGCAAGTTTCGTTGCTGCCGTTAACCCGTCTGGTTTGGGCATTATACTGATGTAGGCACGCTTTATCGTTTTCACGAAGct                 <  2:344877/251‑1 (MQ=255)                                                                                                                                                                                                                      tatTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTCTCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGctc                >  1:227127/1‑251 (MQ=255)                                                                                                                                                                                                                       atTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATCCTGATGTAGTCACGCTTTATCGTTTTccctcagctct               >  2:264676/1‑241 (MQ=255)                                                                                                                                                                                                                          gACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGTTGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTg            >  1:79106/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGtt  >  1:42744/1‑250 (MQ=38)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      GAAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTT  >  NC_000913/1467662‑1468139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.