Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,970 +GT coding (789/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9681.G92.6% 74.6 / 1.6 27coding (787/1632 nt)oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (15/10);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01
*NC_0009131,301,9682.T92.6% 77.7 / 0.9 27coding (787/1632 nt)oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (15/10);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

ATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑GT‑‑AGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGC  >  NC_000913/1301726‑1302196
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                      
ataAAATACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAAGTCGGCGAAAAATGGACCCATCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGTTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTTCC‑‑Gtgtaggg                                                                                                                                                                                                                                <  2:241292/251‑7 (MQ=255)
                       aCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGGCAACCGCGACC‑‑GTGGAGTGGTGAAATCTACATGACGTATAAc                                                                                                                                                                                                         <  2:144499/251‑1 (MQ=255)
                                   ccAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATc                                                                                                                                                                                             <  2:21023/251‑1 (MQ=255)
                                                      aaTTCGCCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAACGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                           >  2:316160/1‑250 (MQ=255)
                                                         gcGGCGAAAAATGGACCCAGCCTGGTACTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAATTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTGCCAGAAGCTGaac                                                                                                                                                                        <  2:359088/249‑2 (MQ=255)
                                                                  aaTGGACCCAGACTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACTGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATcc                                                                                                                                                              >  2:46891/1‑251 (MQ=255)
                                                                    tGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                            >  2:353682/1‑251 (MQ=255)
                                                                                ggTAATATCGTCACCAACGGTGCCTATTCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCGTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATTACGTATAACAGCa                                                                                                                                                                                                     <  2:353918/198‑1 (MQ=255)
                                                                                ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCa                                                                                                                                                                                                     >  1:353918/1‑198 (MQ=255)
                                                                                      atCGTCACCAACGGTGCATATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTTTATTAACCCGGTAACCTATTTGCCTATTGCTTCTTAAGTTACAGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAGTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCTCGTTGa                                                                                                                                           <  2:295618/250‑1 (MQ=255)
                                                                                                  ggTGCCAATACCTTCAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGAAGCCCGACCTACTGGACCAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTAACGATGTCAACCGCTCGCGTGT‑‑AGTGGTGAAATCGACATGACGTCTAACAGCATGCCGATCGACTTGTTCCAGAAGCTGAAAAACGAGAACCCGGCCGAAGTTCACGTTGATCCATACAtgtg                                                                                                                               >  2:285740/1‑250 (MQ=255)
                                                                                                         ataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACt                                                                                                                       >  2:213916/1‑251 (MQ=255)
                                                                                                                 aaaGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGAAATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCACGGCCGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTagga                                                                                                                >  2:13466/1‑247 (MQ=255)
                                                                                                                         ggTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaaca                                                                                                        >  1:289353/1‑250 (MQ=255)
                                                                                                                                   gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                             >  2:81388/1‑251 (MQ=255)
                                                                                                                                   gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                             <  2:73904/251‑1 (MQ=255)
                                                                                                                                              tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                                                   >  2:255200/1‑250 (MQ=255)
                                                                                                                                                ttGAACGCAGCCCGACCTACCGGAACAACGCGAAAACCGTTATTAACCATGTAACCTATTTGCTTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACa                                                                                                                                                                                                        <  1:204280/131‑1 (MQ=255)
                                                                                                                                                ttGAAAGCAGCCCGAACTACTGGAACAACGCGAAAACCGTTATTAACCCGGAAACCTATTTGCCTATTGCTTCTGCAGTTCCCGATGTCCACAGCTACCGTGT‑‑AGTGGTGAAATCGAAATGACGTATaaaa                                                                                                                                                                                                        >  2:204280/1‑129 (MQ=255)
                                                                                                                                                                      gAACAACGCTAAACCCGTTATTAACCAGGTAACCTATTTGCCTATGGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAGTAAGAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGaa                                                          <  2:149872/251‑1 (MQ=255)
                                                                                                                                                                                aaacccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGCAACCGCCATTCAACGATGTGCGTGTGCGAACCGCACTGAAACTAGGTATg                                                >  2:177799/5‑251 (MQ=255)
                                                                                                                                                                                 aaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTACCAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATgg                                               <  1:296478/251‑1 (MQ=255)
                                                                                                                                                                                    ccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                            <  1:213916/251‑1 (MQ=255)
                                                                                                                                                                                      gTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATGGACcgc                                          >  1:341524/1‑251 (MQ=255)
                                                                                                                                                                                                aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTg                                >  2:328796/1‑251 (MQ=255)
                                                                                                                                                                                                     aCCTATTTGCCTATTTCTTCTGAATTTACCGATGTCACCCGCTCCCGTGT‑‑AGTTGTGAAAGCGACATGACGTATAACAGCATGCTGATCGAATTGTTCCAGAAGCTGAAAAAAGATACCCCTGACGAAGTTCAGGTGGATCCATCCCTGTGCACTTTCTATTACGAATTTAACAACCAGAATCCTCCATTCAACGATGTGCGTGTGCGTACCGCACTGATACTAGTTATGGACCGCGATATTAGTGTtaat                           <  2:336583/251‑1 (MQ=255)
                                                                                                                                                                                                                              ttACCGATGTCAACCGCTACCGTGT‑‑AGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGAAGGGCAACATGc  >  1:290355/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                      
ATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑GT‑‑AGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGC  >  NC_000913/1301726‑1302196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑