Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,565	C→T	G169G (GGG→GGA)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,565	0	C	T	100.0%	60.3 / NA	18	G169G (GGG→GGA)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

CAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTA  >  NC_000913/1695461‑1695792                                                                                                         |                                                                                                                                                                                                                                    caactagctgACGCGTGGTTACAGTCGTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATaa                                                                                       <  2:21521/238‑1 (MQ=255)         tgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGCAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTAACACAAACGGTGATACGTACACTTTTCCCGGCAATAAAATACGGCGTg                                                                            >  1:391090/2‑250 (MQ=255)         tgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTg                                                                            >  1:372733/2‑250 (MQ=255)             gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGt                                                                             >  2:423854/1‑245 (MQ=255)             gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGt                                                                             <  1:423854/245‑1 (MQ=255)               gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCAcc                                                                                                                                                                   >  2:330289/1‑157 (MQ=255)               gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCAcc                                                                                                                                                                   <  1:330289/157‑1 (MQ=255)               gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCgg                                                                     >  1:186881/1‑251 (MQ=255)                              cgcgACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTtcgtcg                                                                                                                                                                            >  1:175156/1‑133 (MQ=255)                              cgcgACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAAGCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTgcgtcg                                                                                                                                                                            <  2:175156/133‑1 (MQ=255)                                   cATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt                                                                                                                                                                                         >  1:405362/1‑115 (MQ=255)                                   cATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt                                                                                                                                                                                         <  2:405362/115‑1 (MQ=255)                                        gTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTg                                            <  2:372733/251‑1 (MQ=255)                                                ggTGATATCGTCCACCCAGGTGTTCGTCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATATTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCa                                    <  1:63727/251‑1 (MQ=255)                                                    atatCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACGCTTTTCCCGGCAATAACATACTGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATTCTCCATCAc                                <  2:139656/251‑1 (MQ=255)                                                         gTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCt                           >  1:56479/1‑251 (MQ=255)                                                                              ggtgTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGc      <  1:256601/251‑1 (MQ=255)                                                                                   agagCATTACGCTGCGATGGCTTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTa  >  2:159250/1‑250 (MQ=255)                                                                                                         |                                                                                                                                                                                                                                    CAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTA  >  NC_000913/1695461‑1695792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑