Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	49.4 / NA	19	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/9);  total (10/9)

TTCTGTTTATACTGGAGTTCGCCCT‑GGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTT  >  NC_000913/1652096‑1652552                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              ttCTGTTTCTACTGGAGCTAGCCCTCGATG‑GCTGTGAGCCCTTGGATGGGAGCACATAGATCTGGAACCTGGAACTATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTAACGGTACCAAAAACACAGGCGGGAAGCAATAGGGTTATTCATCTCATTAAGCCAGCACTCAACCACCTCCCGAGTCAGGTGACATTAACGAGAATGAGCAAAACGCCTCTCATTGCTGTTCACCTCAGAGAGTAtagc                                                                                                                                                                                                                 >  2:213728/1‑247 (MQ=255)      tttATACTGGAGTTCGCCCT‑GGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCCTTTCTCTTATTAAGCCAGCAATCGACGCCCTCCGGAGGCAGATGACATTAAAGAGACTGAGCAAAGAGAATATCCTTTATGTTCACCTCAGAGAGTAAGGcagaac                                                                                                                                                                                                            >  2:290039/1‑250 (MQ=255)          taCTGGAGTTCGCCCT‑GGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCAGTCATCTTATTAAGAACGCAAACGACGCCATCCGGAGTACGATGACATTAACGAGAATGAGCAAAGAACATATAATTGATGTTACCCTCCGCGAGTATGGcagaacagaa                                                                                                                                                                                                        >  1:361010/1‑250 (MQ=255)                  ttCGCCCT‑GGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCCGAAAGTATGGCAGAACAGAAAAACaaaa                                                                                                                                                                                                >  2:193569/1‑250 (MQ=255)                             ttaGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGTCATTCATTTTATTAAGGCAGCAAACAACGATCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGGTCACCTCAGAGAGAATGGGAGAACAGAAAAACAAAAAAACACCtttt                                                                                                                                                                                     >  1:6052/3‑250 (MQ=255)                                   gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACCTTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCAACTCCGAGAGTATGGCAGAACAAAAAAACAAAAATGCACCTTTGtttttt                                                                                                                                                                               >  2:176530/1‑250 (MQ=255)                                               aTGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCATCTTTTTAAGCCAGCAATCGACGATCTCCGGAGTCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCCACCTGAAgtgt                                                                                                                                                                   >  1:366199/1‑251 (MQ=255)                                                                   aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt                                                                                                                                               <  2:33840/251‑1 (MQ=255)                                                                                   gatCAGGAGACATTTAGCAAAAGACCTTTTCACGGTACCAAAAACACAGGCGGGAACCAAGCGGGTCATTCATCTCATGAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTGACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAACGTACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGATTAAAAAATTATGGGGACCATTTTACCg                                                                                                                               <  2:79743/251‑1 (MQ=255)                                                                                                      aaaGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCGCAGAGAGTAGGGCAGAACAGAAAAACAAAAAGGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGGGACCATTTTACCGTTGACTCAATAAGGCAGAt                                                                                                            <  1:184547/251‑1 (MQ=255)                                                                                                                     gTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGAt                                                                                             <  1:290039/251‑1 (MQ=255)                                                                                                                                       ggAACCAATCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACAGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGATAAACTAAAATGCACCTTTGTTTTGCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATGTTGCCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGttgctgactccg                                                                           <  2:348917/251‑12 (MQ=255)                                                                                                                                             aaTCGGGTCATTCATCTTATTAAGACAGCAATCGACGCTCTCCGGAGTCAGATGACATCAACGAGACAGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                     >  2:364432/1‑251 (MQ=255)                                                                                                                                                cGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaa                                                                  <  1:404694/251‑1 (MQ=255)                                                                                                                                                      attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGGCATTAACGAGACTGAGCAAAGAGCATAGCAGTGATGTTCACCTCAGAGAGTATGGTAGAACAGAAAAACAAAAATGCACCTTTGTTTGTCAACCTGAAGTGTCAGCTAGAGTAAAAAATTATGGTGACCATTTTACCGGTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCata                                                            <  1:382456/251‑1 (MQ=255)                                                                                                                                                         catCTTATTAAGCAAGCAATCGACGCTCTCCGGAGTCAGATGACATTTACTAGACTGAGCAAACAGCATATCTTTGATGTTCACCTCAGCGAGTATGGCAGAACAGACAACCAAAAATGCACCTTTGTTTTTCAACATGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGAATCAATAAGGCAGATGTGGGATGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc                                                          >  2:104885/1‑250 (MQ=255)                                                                                                                                                              tattaAGCCAGCAAACGACGCTCGCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCATTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAg                                                        <  1:144910/247‑1 (MQ=255)                                                                                                                                                                                       cGGAGTCATATGACATTAACGAGACTGAGCAAAGAGCATTTCATTGATGTTCACCTCAGATAGTATGGCAGAACAGAAAAACAAAAATGCCCCGTTTTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTTACTCAATAAGGCAGATGTGGGACGCAGCGATAAATCGTTCCGGACTCCGCCATCGAAAATCATAGCAGTCGAGACATACTTCTGCCTGCTGGTCGCt                           <  2:361010/251‑1 (MQ=255)                                                                                                                                                                                                                tGAGCAAAGAGCATATCATTGATGTTCACCTCAGAAAGTATGGAAGAACAGAAAAACAAAAATGCCCCTTTGTTTTTCAACCTGAAGTGTAAGCGAGAGTAAAAAATTATGGTGAACATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGAAGCGATAAAACGTGCCGGACTCCGCCATAGAAAATCATATCCGTCGAGACATACTTATGCCAGCTGGACGCTGACAGCTGGTGCTAACCCGGCAttt  >  1:390080/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              TTCTGTTTATACTGGAGTTCGCCCT‑GGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTT  >  NC_000913/1652096‑1652552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑