| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,310,841 | C→T | D9N (GAC→AAC) | yciI ← | protein YciI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,310,841 | 0 | C | T | 93.1% | 87.4 / ‑0.3 | 30 | D9N (GAC→AAC) | yciI | protein YciI |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (11/16); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01 | |||||||||||
TCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACT > NC_000913/1310606‑1311085 | tCATAGACTCCTGCCTCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCTTAAATaa < 1:333808/250‑1 (MQ=255) ggacccgtGCCGCGAAGTACGGGTCCGCATCGGCCCAGGCCTGAGCAGCTTCCAGCGATTCAAATTAAGCAATAACGGTTGAGCCGGTAAGTCCCGCAGCCCCTGGATCGGTGCTGTCTACCGCTGGGAGTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGTCGGACGAACGGAAAGGCGTGTTTCGAGGCTATGAGCGTT‑GTTTTGGGCGTAAATAACATac < 2:213415/244‑1 (MQ=255) aCTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATaca > 2:99387/1‑250 (MQ=255) cTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATacaac > 2:416709/1‑251 (MQ=255) gTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGtt < 1:67051/251‑1 (MQ=255) gCCCACGCCTGAGCAGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGtt > 1:343734/1‑251 (MQ=255) aaTTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGa > 2:294601/1‑247 (MQ=255) ggTTGAGCCAGTAAATTCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAga < 1:50591/251‑1 (MQ=255) gCCAGTAATGCCCGCAGCCCCTGGATCGTTACTGTCTAACGCTGGCATGGTATCCGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTGTGAGCGTAAATAACATACAACACGGGCACAACGCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGt < 1:276604/251‑1 (MQ=255) aGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAACTGACCACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTCTCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGAACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCtt > 1:183920/1‑251 (MQ=255) aaaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGt > 1:199017/1‑251 (MQ=255) aaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGtt < 1:119700/251‑1 (MQ=255) gCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATg < 2:199017/251‑1 (MQ=255) ggATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCt > 2:240976/1‑211 (MQ=255) ggATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GGTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCt < 1:240976/211‑1 (MQ=255) aCCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACAtt < 1:6089/251‑1 (MQ=255) gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACTGAAAGGCGTTTTGCGAGGCTATCAGCTTTTGT‑GTGAGCGTAAATAACATACAACACGGGCACAACGCCTGGTTCGGGAAAGTTGTAAAGTTCGTTATTTGAAAGGGCGAAGATCTGCATCGGAAAGATGATGTCTGTGTGAAGGCCAGGCATAAAGTAAGGCTAAGTACGCCAAAAATGACAGTTTCActg < 2:417406/251‑1 (MQ=255) gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActg < 2:304129/251‑1 (MQ=255) tCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActg > 2:205740/1‑250 (MQ=255) cGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActgatcctgatc < 1:416709/251‑1 (MQ=255) aaCTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAat < 2:141963/250‑1 (MQ=255) tAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGt > 1:90864/1‑118 (MQ=255) tAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGt < 2:90864/118‑1 (MQ=255) gttaaTGTGCCGGACTAAACGAAAGGCGTCTTTCGAGGCTATCAGCTTT‑GTTTTTAGCGTAAATAACAAACAACACGGGCACAACTCCTTGTTCGGGAAAGTTTTAAAGGTCGTTATTTGAAAGGGGGAAGATCTGCAACGGAAAGATGATGGCTTTGTTTAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTAGTGAATATGATTGCTATTTg < 2:370559/248‑1 (MQ=255) cGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCGTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCgag < 1:20489/251‑1 (MQ=255) aCGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGAcc > 1:364311/1‑251 (MQ=255) aGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAAt > 1:391330/1‑251 (MQ=255) aGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATa < 2:174288/138‑1 (MQ=255) aGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATa > 1:174288/1‑138 (MQ=255) tt‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGGAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACt < 1:347801/249‑1 (MQ=255) | TCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACT > NC_000913/1310606‑1311085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |