Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,310,770	A→G	H32H (CAT→CAC)	yciI ←	protein YciI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,310,770	0	A	G	100.0%	124.7 / NA	34	H32H (CAT→CAC)	yciI	protein YciI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

CGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATC  >  NC_000913/1310530‑1311009                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 cGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACt                                                                                                                                                                                                                                        <  1:304074/251‑1 (MQ=255)       cAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCACCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAAc                                                                                                                                                                                                                                  >  1:304129/1‑251 (MQ=255)        agaaacggggaccTTAAATATTGCGGCTCTCTTCAGAACACTTTCTTAAATGGTTTCACTGCCCCTAGTTCATCTCCTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTGCTCCCTAACGGTTGAGCCAGTAAATCCCGCAGCACCTGGATCGTTCCTGTCTACCGCTGGCATTGGACCAGCTGTCACCAAACGACCTTCATCGTGCAGTAACTGTAAACg                                                                                                                                                                                                                                 >  1:175852/12‑251 (MQ=255)                 gCCCTTAAATATTGAACTTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGGGCTAAAt                                                                                                                                                                                                                         <  1:315051/250‑1 (MQ=255)                               aaTCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACgg                                                                                                                                                                                                          >  2:347801/1‑251 (MQ=255)                                               acTTTCTTAAATGGTTTCACTGAAACGAGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCg                                                                                                                                                                                            >  2:20489/1‑249 (MQ=255)                                                       aaaTGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCa                                                                                                                                                                                   <  1:30197/250‑1 (MQ=255)                                                                         gtgttcaaaaagtCCTGCCGCTACGTACGGGTCAGACTCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTAAACCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTTCCGCTGGCATTGGACCAGCTTTCAAAACAAGGCCTTCATCGTGCAGTAACTGTACACGTGCTAAATGTGCCGAACGACCGGAACGGCGTTTTTCGGGGCTATCAGCATT‑gctttgcgagtaa                                                                                                                                                                >  2:363993/13‑238 (MQ=255)                                                                             tCATAGACTCCTGCCTCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCTTAAATaa                                                                                                                                                            <  1:333808/250‑1 (MQ=255)                                                                                 ggacccgtGCCGCGAAGTACGGGTCCGCATCGGCCCAGGCCTGAGCAGCTTCCAGCGATTCAAATTAAGCAATAACGGTTGAGCCGGTAAGTCCCGCAGCCCCTGGATCGGTGCTGTCTACCGCTGGGAGTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGTCGGACGAACGGAAAGGCGTGTTTCGAGGCTATGAGCGTT‑GTTTTGGGCGTAAATAACATac                                                                                                                                                       <  2:213415/244‑1 (MQ=255)                                                                                   aCTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATaca                                                                                                                                                      >  2:99387/1‑250 (MQ=255)                                                                                    cTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATacaac                                                                                                                                                    >  2:416709/1‑251 (MQ=255)                                                                                                       gTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGtt                                                                                                                                 <  1:67051/251‑1 (MQ=255)                                                                                                                 gCCCACGCCTGAGCAGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGtt                                                                                                                       >  1:343734/1‑251 (MQ=255)                                                                                                                                         gATTCAAATTCAGCAATAACGGTTGAGCCAGTACATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCCACAAACGACCTTCCTCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGCCGAACGGa                                                                                                                                                                                                         >  1:270408/1‑146 (MQ=255)                                                                                                                                         gATTCAAATTCAGCAATAACGGTTGAGCCAGGAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCGTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCTTACGAACGGa                                                                                                                                                                                                         <  2:270408/146‑1 (MQ=255)                                                                                                                                               aaTTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGa                                                                                             >  2:294601/1‑247 (MQ=255)                                                                                                                                                             ggTTGAGCCAGTAAATTCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAga                                                                           <  1:50591/251‑1 (MQ=255)                                                                                                                                                                   gCCAGTAATGCCCGCAGCCCCTGGATCGTTACTGTCTAACGCTGGCATGGTATCCGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTGTGAGCGTAAATAACATACAACACGGGCACAACGCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGt                                                                     <  1:276604/251‑1 (MQ=255)                                                                                                                                                                      aGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAACTGACCACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTCTCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGAACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCtt                                                                  >  1:183920/1‑251 (MQ=255)                                                                                                                                                                         aaaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGt                                                               >  1:199017/1‑251 (MQ=255)                                                                                                                                                                          aaTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGtt                                                              <  1:119700/251‑1 (MQ=255)                                                                                                                                                                                  aGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACg                                                                                                                                                                                                               >  1:54738/1‑99 (MQ=255)                                                                                                                                                                                  aGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCGTCATCGTGCAGTAACTGTAAACTTGCTAAAGGTGCCGGACg                                                                                                                                                                                                               <  2:54738/99‑1 (MQ=255)                                                                                                                                                                                   gCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATg                                                     <  2:199017/251‑1 (MQ=255)                                                                                                                                                                                         ggATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCt                                                                                       >  2:240976/1‑211 (MQ=255)                                                                                                                                                                                         ggATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GGTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCt                                                                                       <  1:240976/211‑1 (MQ=255)                                                                                                                                                                                                      cTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑Gtt                                                                                                                                                                          >  1:93896/1‑114 (MQ=255)                                                                                                                                                                                                      cTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑Gtt                                                                                                                                                                          <  2:93896/115‑2 (MQ=255)                                                                                                                                                                                                                      aCCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACAtt                  <  1:6089/251‑1 (MQ=255)                                                                                                                                                                                                                             gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACTGAAAGGCGTTTTGCGAGGCTATCAGCTTTTGT‑GTGAGCGTAAATAACATACAACACGGGCACAACGCCTGGTTCGGGAAAGTTGTAAAGTTCGTTATTTGAAAGGGCGAAGATCTGCATCGGAAAGATGATGTCTGTGTGAAGGCCAGGCATAAAGTAAGGCTAAGTACGCCAAAAATGACAGTTTCActg           <  2:417406/251‑1 (MQ=255)                                                                                                                                                                                                                             gTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActg           <  2:304129/251‑1 (MQ=255)                                                                                                                                                                                                                              tCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActg           >  2:205740/1‑250 (MQ=255)                                                                                                                                                                                                                                      cGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTTTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActgatcctgatc  <  1:416709/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 CGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTT‑GTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATC  >  NC_000913/1310530‑1311009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑