Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT96.0% 81.1 / ‑6.7 25N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (10/14);  minor base C (1/0);  total (11/14)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑G‑‑TAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCG  >  NC_000913/1301753‑1302219
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                             >  1:422991/1‑250 (MQ=255)
 aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACa                                                                                                                                                                                                                               >  2:357038/1‑247 (MQ=255)
 aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACa                                                                                                                                                                                                                               <  1:357038/247‑1 (MQ=255)
            aaGCCGCTATCGAGACATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAt                                                                                                                                                                                                                >  1:99972/1‑251 (MQ=255)
            aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGAATGCCGATCGAAt                                                                                                                                                                                                                >  2:101720/1‑251 (MQ=255)
            aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTGACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAt                                                                                                                                                                                                                <  2:322005/251‑1 (MQ=255)
                                                             cGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATcc                                                                                                                                                               >  1:329919/1‑251 (MQ=255)
                                                                 cccAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAATCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                                           <  1:174476/250‑1 (MQ=255)
                                                                   cAACGGTGCCTATACCTTAAAAGTTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAGCCAGGTAACCTATTTGCCTATTGCTTCTGAAGGTACCGATGTCAACCGCTACC‑‑GGGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt                                                                                                                                                         <  1:334883/251‑1 (MQ=255)
                                                                              ataCCGTAAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACt                                                                                                                                              <  2:296453/251‑1 (MQ=255)
                                                                                 ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAtt                                                                                                                                           >  2:75429/1‑251 (MQ=255)
                                                                                            tGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaa                                                                                                                                 <  2:82778/250‑1 (MQ=255)
                                                                                                    aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTTCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATACCTGTGCATTTACTATTTCGAAATGAACAACCAgc                                                                                                                         <  2:111203/250‑2 (MQ=255)
                                                                                                    aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGa                                                                                                                         <  1:75429/250‑1 (MQ=255)
                                                                                                        gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                                    <  1:322047/251‑1 (MQ=255)
                                                                                                                                                    gAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt                                                                        >  1:47837/1‑251 (MQ=255)
                                                                                                                                                             tattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                <  2:99972/250‑1 (MQ=255)
                                                                                                                                                                      ggTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACTGCGATATCATTGt                                                      <  1:20555/251‑1 (MQ=255)
                                                                                                                                                                       gTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGCATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCACCGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt                                                     >  2:79958/1‑251 (MQ=255)
                                                                                                                                                                                  gcctttttcTTCTGAAGTTTCTGATGTCAACCGCAACCGTG‑‑TAGTGGGGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTAACTTTTTCGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGTGATATCATTGTTAATAAAGTGaa                                          <  2:252845/243‑1 (MQ=255)
                                                                                                                                                                                           ttCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAggg                                 >  2:144649/1‑251 (MQ=255)
                                                                                                                                                                                               gAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGATATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCa                               <  1:332021/249‑1 (MQ=255)
                                                                                                                                                                                                          tGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt                   >  1:266667/1‑250 (MQ=255)
                                                                                                                                                                                                           gTCAACCGCTACCGTG‑‑TAGTGGTGCAATCGACATGAAGTACAACATCATGCTGATGGAATTTTTCCAGAAGCTGAAAAAAGAGACCCCGGACAAAGTTCACGTTGATCCATGCTGGTGCCTTTATTATTACAAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACGAGGTATGGACCGCGATATCTTTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa                  <  2:257821/250‑1 (MQ=255)
                                                                                                                                                                                                                          g‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg  >  2:423053/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑G‑‑TAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCG  >  NC_000913/1301753‑1302219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑