Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,291,496	C→T	P14S (CCC→TCC)	galU →	UTP‑‑glucose‑1‑phosphate uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,291,496	0	C	T	100.0%	109.8 / NA	30	P14S (CCC→TCC)	galU	UTP‑‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

GAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCCCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGC  >  NC_000913/1291250‑1291727                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        gAATGAGCAAACGATAACGCGGGCTAAAGTTTCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGt                                                                                                                                                                                                                                     <  2:345802/251‑1 (MQ=255)                      ggcgaaaTTTGCATTACCTGATAATGTCGGCCGGTGGAACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACACGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAGACACGAACAGTCCAAGAGAATTTAAATGGCTACCATTCAAACGAAAGTCAAATAAGACGTTATCTCCGATGCGGGATTAGGAAACAGGa                                                                                                                                                                                                                 >  2:92804/5‑250 (MQ=255)                                       cTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATACCTAATTGTCTCACTTGTGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATACCCTGGGATGCGATACGGAAATATGAACACGTTCAAAACACTACCAGTCCAGGAGACTTTAAATGGCTGCCATTAATACGAAAGTCACAAAAGCCGATATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGaac                                                                                                                                                                                                >  1:48063/1‑249 (MQ=255)                                                   gCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCTCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTGGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAag                                                                                                                                                                                   <  1:388780/251‑1 (MQ=255)                                                      ggtggtACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAaga                                                                                                                                                                                  <  1:299090/249‑1 (MQ=255)                                                                       cATTCGAATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCTCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCAGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCgct                                                                                                                                                               >  2:363287/1‑249 (MQ=255)                                                                         ttCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGATGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACAAGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGAGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATa                                                                                                                                                              >  1:319501/1‑250 (MQ=255)                                                                           cGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAg                                                                                                                                                            <  2:283805/250‑1 (MQ=255)                                                                                 aGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCGTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAAGTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGGTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAATGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTaa                                                                                                                                                     <  1:419572/251‑1 (MQ=255)                                                                                            tAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATAc                                                                                                                                             <  1:252082/248‑1 (MQ=255)                                                                                              aTTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGAGGCTGCCACTTGTCGATAAGCCATTAATTCAATAcgtcgt                                                                                                                                        >  2:394066/1‑251 (MQ=255)                                                                                                               cctTTTCCGAACTTAGCCCCTTCGGGGCGCTGATATACTGGGATGCGACACAGAAATATGAACACGTTCAAAACACGCACAGTCCAGGAGAATTTAAATGGCTGCCATTAATAAGAAAGTCACAAAAGCCGTTATCTCCATTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAACGCCTTCCCGACAGAGATGCTGCCACTTGTCGCTAAGCCATTAATTAAATACGTCATGATTGAATGTATTGCgg                                                                                                                       >  2:358226/1‑251 (MQ=255)                                                                                                                 ttttCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTACATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGATTGAATGTATTGCGGCt                                                                                                                     >  2:362119/1‑251 (MQ=255)                                                                                                                                cccTTCGGGGGGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAAGTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAAtt                                                                                                      <  2:243546/251‑1 (MQ=255)                                                                                                                                      ggggTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTg                                                                                                >  1:364103/1‑251 (MQ=255)                                                                                                                                       gggTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTgg                                                                                               >  2:166507/1‑251 (MQ=255)                                                                                                                                           gCTGACATTCTGGGGTGCGATACAGAAATATGAACACGTTCACAACACGAACAGTCAAGGAGAATTTAAATGGCTGCCATTAATTAGAAAGTCAAAAAAGCAGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCACGAAAGAGATTCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTac                                                                                           >  2:336780/1‑251 (MQ=255)                                                                                                                                                   aCTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCAt                                                                                   >  2:309258/1‑251 (MQ=255)                                                                                                                                                           gCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAAc                                                                           <  1:130167/251‑1 (MQ=255)                                                                                                                                                                     aaTATGATCACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGTTGACATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGTAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACTCTCATCTAAAAACTCTATTGaaa                                                                 <  2:259562/251‑1 (MQ=255)                                                                                                                                                                           aaCACGCTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACt                                                           >  1:109189/1‑251 (MQ=255)                                                                                                                                                                                     aaaCACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAACCCACTTTGATAcc                                                   >  2:154491/1‑249 (MQ=255)                                                                                                                                                                                                gTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTgg                                      <  1:154491/251‑1 (MQ=255)                                                                                                                                                                                                                       ccATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGACGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgagt                >  2:260773/1‑247 (MQ=255)                                                                                                                                                                                                                         aTTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGcc                                                                                                                                                          <  2:403601/110‑1 (MQ=255)                                                                                                                                                                                                                         aTTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGAGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGcc                                                                                                                                                          >  1:403601/1‑110 (MQ=255)                                                                                                                                                                                                                           tAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAAc           >  1:11524/1‑251 (MQ=255)                                                                                                                                                                                                                              tACGAACGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTAGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAACCTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACg                   >  2:278025/1‑240 (MQ=255)                                                                                                                                                                                                                              tACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACg                   <  1:278025/240‑1 (MQ=255)                                                                                                                                                                                                                                    aGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGc  <  1:107530/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        GAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCCCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGC  >  NC_000913/1291250‑1291727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑