| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,301,970 | +GT | coding (789/1632 nt) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,301,968 | 1 | . | G | 92.0% | 83.7 / 0.4 | 25 | coding (787/1632 nt) | oppA | oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (10/13); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| * | NC_000913 | 1,301,968 | 2 | . | T | 92.0% | 74.7 / 0.4 | 25 | coding (787/1632 nt) | oppA | oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (10/13); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
ATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑G‑‑TAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA > NC_000913/1301726‑1302203 || ataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTAGCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCGATTTGCCTATTGCTGCTGAAGTTACCGATGTCAACCGCTACC‑‑G‑‑tgta < 1:239462/248‑4 (MQ=255) cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGc > 1:422991/1‑250 (MQ=255) aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACa > 2:357038/1‑247 (MQ=255) aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACa < 1:357038/247‑1 (MQ=255) aaGCCGCTATCGAGACATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAt > 1:99972/1‑251 (MQ=255) aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGAATGCCGATCGAAt > 2:101720/1‑251 (MQ=255) aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTGACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAt < 2:322005/251‑1 (MQ=255) cGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATcc > 1:329919/1‑251 (MQ=255) cccAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAATCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc < 1:174476/250‑1 (MQ=255) cAACGGTGCCTATACCTTAAAAGTTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAGCCAGGTAACCTATTTGCCTATTGCTTCTGAAGGTACCGATGTCAACCGCTACC‑‑GGGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt < 1:334883/251‑1 (MQ=255) ataCCGTAAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACt < 2:296453/251‑1 (MQ=255) ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAtt > 2:75429/1‑251 (MQ=255) tGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaa < 2:82778/250‑1 (MQ=255) aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTTCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATACCTGTGCATTTACTATTTCGAAATGAACAACCAgc < 2:111203/250‑2 (MQ=255) aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGa < 1:75429/250‑1 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg < 1:322047/251‑1 (MQ=255) gAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt > 1:47837/1‑251 (MQ=255) tattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg < 2:99972/250‑1 (MQ=255) ggTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACTGCGATATCATTGt < 1:20555/251‑1 (MQ=255) gTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGCATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCACCGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt > 2:79958/1‑251 (MQ=255) gcctttttcTTCTGAAGTTTCTGATGTCAACCGCAACCGTG‑‑TAGTGGGGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTAACTTTTTCGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGTGATATCATTGTTAATAAAGTGaa < 2:252845/243‑1 (MQ=255) ttCTGAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAggg > 2:144649/1‑251 (MQ=255) gAAGTTACCGATGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGATATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCa < 1:332021/249‑1 (MQ=255) tGTCAACCGCTACCGTG‑‑TAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt > 1:266667/1‑250 (MQ=255) gTCAACCGCTACCGTG‑‑TAGTGGTGCAATCGACATGAAGTACAACATCATGCTGATGGAATTTTTCCAGAAGCTGAAAAAAGAGACCCCGGACAAAGTTCACGTTGATCCATGCTGGTGCCTTTATTATTACAAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACGAGGTATGGACCGCGATATCTTTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa < 2:257821/250‑1 (MQ=255) || ATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACC‑‑G‑‑TAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA > NC_000913/1301726‑1302203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |