Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,699,275	C→T	intergenic (‑95/‑80)	malI ← / → malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,699,275	0	C	T	100.0%	32.2 / NA	13	intergenic (‑95/‑80)	malI/malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

AGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGAT  >  NC_000913/1699079‑1699492                                                                                                                                                                                                     |                                                                                                                                                                                                                          agagATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCa                                                                                                                                                                      <  2:123752/250‑1 (MQ=255)        cGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAAGGTGATTTATGTCTCACTAAAATTTGTTAAACCGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTTTtc                                                                                                                                                              >  1:105303/1‑251 (MQ=255)                gCCACTAAGCACAAGAGAAACGGTCCTTACCGACACGCCCGCAGCCAGCTCAACAGCATGAAGGGTTATTTTTGTGGCGGTAGCCATGCAGATGACCTACTCCCTTATTATGTGACAGATAAAACGTTTTACCTTTTATTTATATTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAAGTTGATAAATCGTTTGATCGTCTCGCGAAATGTACTGAATCAAGATTGTTCTCGACgag                                                                                                                                                      <  2:63087/251‑1 (MQ=255)                                   aCGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCGTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCg                                                                                                                                   <  1:268983/251‑1 (MQ=255)                                                                           gAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCcagc                                                                                           <  2:271702/251‑1 (MQ=255)                                                                                gTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGtt                                                                                       >  1:61877/1‑250 (MQ=255)                                                                                                 aGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGt                                                                      <  1:292935/250‑1 (MQ=255)                                                                                                                          aTTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGc                                            >  1:16966/1‑251 (MQ=255)                                                                                                                                 gACAGAGAAAACGTTTTTCCGTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAGAATTTTATAAAACGTTTTATCTTCTCGCGCAATTTACTGAACCCAGAGTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTCAGGCAAACGCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCAtt                                      <  2:294975/250‑1 (MQ=255)                                                                                                                                                            tatCTTATACCCGCTCTTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTagc           >  2:98970/1‑250 (MQ=255)                                                                                                                                                             atCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCCTTATGCTCGGCATTGGTAGTTCTCTTagc           >  2:326915/1‑249 (MQ=255)                                                                                                                                                               cTTATACCCGCTATTATTGTTGCGTAATGTGATTTATGTTTCACTAAAATTTTATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCATATTGTTCTCTACGAGGAGTCGTTTTATGACGGCTAAAACAGCACCGAAAGTCTCGCTGTGGGATTTCTGCCAGCATTTAGGCAAAACTTTCAGGTTACCGGTTGCATTATGGTCTTTCTGCGGCATTAGGCTCGGCATTGGTAGTTCTCTTagca          <  2:249958/248‑1 (MQ=255)                                                                                                                                                                    aCCCGCTATTATAATTGCGCAATGAGAATTATGTCTCCATAAACTTTGCAAAAACGTTTAATATTATAGCGCAATTTACTGAATCAAGAATGTACTCTAAGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGATGTGGGAGTTCTTCCAGCCGTTAGGCAAAACCTTCATGTTACCAGTGGCATTATTGTCGTTCTGCGGAATTATGCTCGGCATTGGAAGTTCTCTTagaggcgctgat  >  2:30636/1‑241 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                          AGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGAT  >  NC_000913/1699079‑1699492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑