Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,565	C→T	G169G (GGG→GGA)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,565	0	C	T	90.0%	28.3 / ‑5.6	10	G169G (GGG→GGA)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/3);  minor base A (0/1);  total (6/4)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAA  >  NC_000913/1695485‑1695810                                                                                 |                                                                                                                                                                                                                                                      tCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAACTCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATa                                                                                                                               >  2:109717/1‑201 (MQ=255) tCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACAGTGATa                                                                                                                               <  1:109717/201‑1 (MQ=255)         gACATGCGTCAACACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGAAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGGTCAGTTCGTTGTTCACACAACCGGTGATATGTAAACTTTTCCCGGCAATAACATACGGCGAGACATAGGCTTCAAATGGCGtatac                                                                      >  2:327454/1‑249 (MQ=255)                   accacGGTGATATCGTCCACCCAGGTGTTCGGCGAGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATAATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATg                                                           >  1:271576/1‑251 (MQ=255)                                        cAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCTCACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACa                                                                                           >  2:217254/1‑198 (MQ=255)                                        cAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACa                                                                                           <  1:217254/198‑1 (MQ=255)                                           gtgtTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAc                                   <  2:39892/251‑1 (MQ=255)                                               tcggctgggtgTAGAGCATTACGCTGCGATGGATACCGGCTTAGTTAAAGAAAGCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCTTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAAGGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGTCCca                                <  2:354355/243‑1 (MQ=255)                                                                    cGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGAGTGACATCGGGTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGAcc          >  2:84373/1‑251 (MQ=255)                                                                             ggATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGAATGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGaa  >  1:279416/1‑250 (MQ=255)                                                                                 |                                                                                                                                                                                                                                                      TCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAA  >  NC_000913/1695485‑1695810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑