Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	41.5 / NA	14	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

TATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652103‑1652571                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   tataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaa‑cag                                                                                                                                                                                                                              <  1:132072/250‑1 (MQ=255)  ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCCCCTCAGAGAGTATGGcagaa‑cagaa                                                                                                                                                                                                                            <  2:93259/251‑1 (MQ=255)           ttCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAACAATGGAACAATGATGAACAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGAAATAGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGGTGTTCACCTCAGCGAGTAGGGCAGAA‑CAGAAAAACaaaa                                                                                                                                                                                                                    >  1:165688/1‑250 (MQ=255)           ttCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGCAACCAA‑TCGGGGCTCTTATCTTTTTAAGCCAACAATTCACGCCCTCCGGAATCAGAATACCTTAACGAGACTCGGCAAAGAGCATTACATTTACTTTTACCTCAAAGAGTATGGCAGAA‑CCGGAAACCaaaa                                                                                                                                                                                                                    >  1:99570/1‑250 (MQ=255)             cGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATg                                                                                                                                                                                                                 <  1:396150/251‑1 (MQ=255)                                       aTGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgtgt                                                                                                                                                                                       <  1:303315/251‑1 (MQ=255)                                                                        gatgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCAGTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTa                                                                                                                                                     <  2:15697/251‑1 (MQ=255)                                                                                   gaAATTTAGCAAAAGACCGTTTCACGGTACCAAAACCAAAGGCGGGAACACA‑TACGGTAATTCATATTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACCTTAAAGAAACTGAGCAAAACGCCTATCATTGATGTTCAACTCAGAGAGAATGGCAGAA‑CAGAAAAACAAACAAGCACCTTTGTTTTTCAACCTGAAGTGTACGCGAGAGAAAAAAATTATGG‑GACCCATTTTCACGTTGACta                                                                                                                                           >  1:384651/1‑249 (MQ=255)                                                                                          aGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAACACGGG‑CATTTATCCTATTAAGGCAACAATCGACGCGCCCCGGAGTCCCATGACAATAAAGAGACTGCGCAAAAAGCATACCATTTATGTTCCCCCCAGAGAGCATGGCAGAAACA‑CAAAACAAAAAAGCCCCTTTGTTTTTCAAACTGAAGTGTCAGAGAGAGGAAAAAATTATTGTGAAC‑CTTTTACCGTTGGCTCAAAAAggg                                                                                                                                   >  1:28587/1‑250 (MQ=255)                                                                                                                             cGGGAACCAA‑TCGGGACATTCATCTTATTAAGCCAGCAATCGACGCTCTCAGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTAACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGAGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACACAATAAGGCAGATGTGGGACGCAGCGATACAACGTGCCGGACTc                                                                                                >  2:402328/1‑251 (MQ=255)                                                                                                                                gAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGc                                                                                             >  1:278303/1‑251 (MQ=255)                                                                                                                                                                                                   cGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaaa                                                                                    >  1:303362/1‑194 (MQ=255)                                                                                                                                                                                                   cGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaaa                                                                                    <  2:303362/194‑1 (MQ=255)                                                                                                                                                                                                                            tGATGTTCACCTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGcc  >  1:265309/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAA‑CAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACC‑ATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652103‑1652571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑