Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	54.4 / NA	23	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

CAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTA  >  NC_000913/1337152‑1337624                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       cAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                <  1:109878/251‑1 (MQ=255)        gCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcg                                                                                                                                                                                                                         >  1:186053/1‑251 (MQ=255)               tccGCAGGCTTGCTGGCGAAAAAAGCCGTACCTCTGGGCCTAAAGCGGCAACCCTGGGGCAAAACGTCGCTGGCACCGGGTTCGAAAGTCGTTTATGATTATCTGGCAAAAGCGAAACTGAAACCGCATCTAGACGAACTGCGGTTTAGCCTTGTGAGAAACGGTTGTACCAACTGTCTAGGTAACTCTTGTCCGCAGCCCGGACCTATCGAAACGGCAATCAAAAAAGGAGATTTAACCggcgtggcg                                                                                                                                                                                                                    >  2:266855/2‑241 (MQ=255)                 cGCAGGCTTGCTGGCGAACAAAGACGAAACTCCGAGCCTCAAGCGGCAACAATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCACAAGCGAAACTGACACCGTATCTCGACGACCTGGGGTTTAAGCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGAGCCGCTGACCGATCCTATCGAAACGGCACTCACACAAGGCGATTTAACCGTcggtgcgtggc                                                                                                                                                                                                                >  2:128973/1‑247 (MQ=255)                    aGGCTTGCTGGCGGAAAAAGCCGTACCTCTGGGCCTCACGCGGCAACCATGGGTCAAAACGTAGCTGGCACCGGGTTAGAAATTCGTTTCTGATTATCTGGCAAAAGCGAAACTGTCACCGGATCTCGACGCACTGGGGTTTAACCTCGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGAACCTCCCGAAACGGCCATCAAAAAAGGCTGTTTAAACGTCGGCGCGGGGCTGt                                                                                                                                                                                                             >  2:386106/1‑251 (MQ=255)                       cTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAACAAAGGCGCTTTAACCGTCGGTGCGGTGCTGTCCg                                                                                                                                                                                                          >  2:145930/1‑251 (MQ=255)                                             aCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGCAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGATGCCCACTCCTATCGAAACGGCCATCAAAAAAGGCGCTTTACCAGTCGGTGCGGTGCTGTCCGTCAACCGTCACttttaccgcct                                                                                                                                                                                    >  2:79793/1‑243 (MQ=255)                                                   ggCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAACGCGAAACTGCCACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGGGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccc                                                                                                                                                                              >  2:132676/1‑250 (MQ=255)                                                               cAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTa                                                                                                                                                                   >  2:342189/1‑250 (MQ=255)                                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                                   >  1:267542/1‑251 (MQ=255)                                                                                                 cGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGCCACCGTATCTCGACGCCCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGtt                                                                                                                                >  1:255532/1‑251 (MQ=255)                                                                                                  gAAAGTCGGTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACGAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                                                                                                               <  1:260751/251‑1 (MQ=255)                                                                                                    aaGTCGTTTCTGATTATTTGGCAAAAGCGAAACTGACACCGTATCTCGAAGAACTGGTGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATACTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCGGGCCTCGCCGCCGCTGGTGGTTGcc                                                                                                                             <  2:267542/251‑1 (MQ=255)                                                                                                                               cGAAACTGCCACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACAACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                                                                                                              >  1:32984/1‑239 (MQ=255)                                                                                                                               cGAAACGGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATGGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTCTCCTGCAGCCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGGCGCCTATGCGCTGGCGGGa                                                                                                              <  2:32984/239‑1 (MQ=255)                                                                                                                                          ccGTATCTCGACGACCTGTGGTTTAACCTTGTGGGATTCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGGCGATTTATCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGGATCCTTCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCGTTTTCGCGGGCTGTAAATATGAATATTAACCTGGCTTc                                                                                       <  2:255532/251‑1 (MQ=255)                                                                                                                                                                                                    gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCa                             <  1:342189/251‑1 (MQ=255)                                                                                                                                                                                                            gggCCGCTGCCCGAGCTGATCGAATCGGCAATCAAAAAAGGCGATGTAACCGTCGGGGCTGTGCTGGCCGGCAACCGTATCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGTAAAGATGAATATCAACCTGGCTTCTGAGCCTGTCGGCCATGATCGCAAAGGCGATCTGGTTTATCTGAAAGATATCTGGCCATCGGcaca                     <  2:170848/251‑1 (MQ=255)                                                                                                                                                                                                                 ccTGCCCGATCCTATCGAAACGTCCACCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCACCCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATTAATATCAACCTGTCTTCTTAGCCTATCGGCCATGATTGCAAAGGCTATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACCAGaaa                <  2:234802/250‑1 (MQ=255)                                                                                                                                                                                                                          tCCTACCGAAACGGCAATCAAAAAAGGCTATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTATCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTATCTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCAGGATCGCAAATGCGATCCTGTTTATCTGAAAGATATCTGGCCAGCGGCACAAGAAATTGCCCGt        <  2:407780/250‑1 (MQ=255)                                                                                                                                                                                                                               gcGAAACGTCAATCAAAAAAGGGGATTTAACCGTCGGTGCGGTGCGGGCCGGTAACCGGAACGTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGTCTGGCCGCGCCGCCGCTGGTTGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCTTATCGGCCATGCTCGCAAAGGCGATCCGGTTGATCTGAAAGATATCTGGCCATCGTCACAAGAAATTTCCCTGGCGGTa  <  2:165579/250‑1 (MQ=255)                                                                                                                                                                                                                                           tCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTTGCTTCTGAGCCTATCGGCCATGATCg                                                                  <  2:37478/175‑1 (MQ=255)                                                                                                                                                                                                                                           tCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCg                                                                  >  1:37478/1‑175 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTA  >  NC_000913/1337152‑1337624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑