Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,519	G→A	A331V (GCG→GTG)	lacI ←	DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,519	0	G	A	100.0%	34.1 / NA	13	A331V (GCG→GTG)	lacI	DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/11);  total (2/11)

AGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCG  >  NC_000913/366401‑366762                                                                                                                       |                                                                                                                                                                                                                                                    gatgcggtcctcaatcgcgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                                                                                                                 <  1:55447/233‑1 (MQ=255)                                 gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCtt                                                                                   <  1:418788/249‑1 (MQ=255)                                 gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCg                                                                                 <  1:283611/251‑1 (MQ=255)                                     gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGGGGGTTTTTTTTTTTCCAGTGAGACGGGCAACAGCTGATTTCCCTTCACCCCCTGGCCCTGAGGGAGGTGTAGCAAGCGGGCCACGCTGGGTTGCCCCCGCCGGCGGAAATCCTGTTTTATGGTGGTTAAAGGCggggggtaaacataggttgtttttgggt                                                                             >  1:164438/1‑226 (MQ=255)                                          ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGAAtcgtc                                                                         <  1:108419/250‑1 (MQ=255)                                                 ggAACCCTGTCGTGCCAGCTGCATTAATGAAACGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAGGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCa                                                                 <  1:53828/251‑1 (MQ=255)                                                      ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTAcc                                                            <  1:210372/251‑1 (MQ=255)                                                         gTCGTGCCTGCTGCATTAATGAATCGGCCAACGCGCTTGGAGAGGCGGTTTGCGTATTGGTAACCAGGTTGGTTTTTCTTTTCACCAGTGATACGGGCAACAGCTTATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTGGCCTCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACGTGAACTGTCGTCGGTAGCTTCGTATCCCACTGCCgac                                                         <  2:276187/251‑2 (MQ=255)                                                                       aTTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGTGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAAc                                           <  1:404389/251‑1 (MQ=255)                                                                             gaagcGGCCAACGGGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGTTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCTCCAACGCGCAg                                     <  2:392664/247‑1 (MQ=255)                                                                             gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAg                                     <  1:358679/251‑1 (MQ=255)                                                                                                   agGCGATTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGAGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATTTAACATGAGCCGTCTTTGGTATCCTCGTATCCCACTAACCAGATATCCGCACCAACGCGCAGCCCCGACTCGGGAAttgcccgc               >  1:135231/1‑244 (MQ=255)                                                                                                                aTTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCACAgcg  <  1:118863/251‑1 (MQ=255)                                                                                                                       |                                                                                                                                                                                                                                                    AGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCG  >  NC_000913/366401‑366762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑