| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,360,528 | T→C | A469A (GCA→GCG) | cadC ← | DNA‑binding transcriptional activator CadC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,360,528 | 0 | T | C | 85.0% | 39.4 / 2.5 | 20 | A469A (GCA→GCG) | cadC | DNA‑binding transcriptional activator CadC |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (9/8); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-02 | |||||||||||
ATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGG‑AGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCAATTCCGGCAGTGTAACAATGTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATG > NC_000913/4360287‑4360776 | atataataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGTAACATACAAAAACTCAACAACAAATAGTGCCGAGCATAAATCAACCCGGGAGTTACTTATTCTGAAGCGAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCTTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTccc < 2:292841/247‑1 (MQ=255) aCCAACAGTAACATACAAAAACTCAACAACAAATATTGCCGAGCATAAATCAACCCGGGAGTTACTTATTCTGAAGCGAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAAtt > 1:203745/1‑251 (MQ=255) cAACAGTAACATACAAAAACTCAACAACAAATATTGCCGAGCATAAATCAACCCGGGAGTTACTTATTCTGAAGCGAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAAttt > 1:242497/1‑250 (MQ=255) tgacGAGCATAAATCAACCCGGGAGTTACTTATTCTGAAGCTAGAAATTTGTAGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAACATTTTCAATACAGTAAAGGGTGTTTGCCCCTTGGCGTAAATTAAAGGCGGTGAGATATGTATCAGCCGCTTCGCGGTTCATCCCCTGCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATCTCAAGAGCAATTCTAGTATTTa < 2:257123/247‑1 (MQ=255) ccggg‑AGTTACTTATTCTGAAGCGAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGc > 2:297067/4‑186 (MQ=255) ccggg‑AGTTACTTATTCTGAAGCGAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGc < 1:297067/183‑1 (MQ=255) cctg‑AGTTACTTAGTCTGAAGCAAGAAATGTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATGTATCGCCTGGTAAGACTCATCt < 2:395765/248‑1 (MQ=255) cGG‑AGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTg > 1:2448/1‑251 (MQ=255) aTAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTATTAttt < 1:110128/251‑1 (MQ=255) aaGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCTCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGc < 1:364041/141‑1 (MQ=255) aaGGTAAAACATAAGGAACAGAAGTCTGGAATATCCCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCTCTTCATTTCATAAACCTTGc > 2:364041/1‑141 (MQ=255) acaacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTACGACTCATCTGTTTTACCTTTTACCAGAGCACt > 2:117606/1‑230 (MQ=255) acaacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAAAGCACt < 1:117606/230‑1 (MQ=255) aGGAACAGAAGTCTGGAATATTCCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTTGGCGTAAATTAAAGGCGGTTAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAATAAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGCCCGCTTTTAATTGATATATAATGGACa < 2:198569/251‑1 (MQ=255) cATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCCGAGCACTGACCGCTTTTATTTGAtatata > 2:161359/1‑221 (MQ=255) cATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGAtatata < 1:161359/221‑1 (MQ=255) aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCAATTCCGGCAGTGTAACAATGt > 1:107010/1‑251 (MQ=255) gTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCAATTCCGGCAGTGTAACAATGTTATCt > 1:162849/1‑250 (MQ=255) tGGGCGTAAATTAAAGGAGGGGCGATATGCATCAGCCGCTTCCCGGTTTATACCCTTCATTACATAAACCTTGCCAAGAAACACATAATTTAGCCAGGACCTTTCAAGATCAATACCAGTATTTATAGCCTGGTAAGACTCATCTGTTTTACCTTTTACCCGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCACTTCCGGCAGTGTAAAAATGTTATCTCTTTCTGTGTTCAGtgctg > 2:336012/1‑251 (MQ=255) aaaTTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCAATTCCGGCAGTGTAACAATGTTATCTATTTCTGTGTTCAGTGCTGCTAATTg > 1:138927/1‑251 (MQ=255) gctgctTACCGGTTGATCCCCTTCATTTCATAAACCTTGCCAAGCAACACCTAATTTAGCCCGGCCATATCAATATCAATGCCAGTATTTATCGCACGGTAAGCATCATCTGTTTTACCTTTTCCCAGAGCACTGACCGCTTTTATTTGATATATAATGGACCGGATGTTCAATTCCGGCAGAGTACCTATGTTATCTATTTCTGTGTACAGTGCTCCTCATTGTTTTTCATCTAAAGGATGTTGCGAAtt > 2:363468/1‑250 (MQ=255) | ATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGG‑AGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTTGTTCAATTCCGGCAGTGTAACAATGTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATG > NC_000913/4360287‑4360776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |