Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,354,4170TG74.2% 47.7 / 15.9 31R101R (AGA→CGA) lysUlysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base G (11/12);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTGCCGCCAGTTTTTCGCG  >  NC_000913/4354178‑4354651
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
ggATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTTCTGATCCTGCAGACCATGGAATTGATCTGGTAAAGGGCGTAGTGCTTTAGTCAGCAGGCGCAGCGCAGTACAGTGAATGGAAAGCTCATCCGTTTGCGTCTGAAACAGCGTACCGCGGGCACCGATAATGTCTCCCAGATCCCATTTTTTAAAGTGATCGTTATAAACAGCTTCTGGCAGGCTATCTCGTGCAACGTACa                                                                                                                                                                                                                                 <  1:258631/251‑1 (MQ=255)
    ttATCGATAGCGATGAGGTCCAGATCACGGTGACGATAACGGACTTCCTGATCCTGCAGGCCACGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTAACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACTGTTg                                                                                                                                                                                                                             >  2:61820/1‑251 (MQ=255)
               gATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAc                                                                                                                                                                                                                  >  1:312310/1‑251 (MQ=255)
                     gTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACAt                                                                                                                                                                                                            >  1:95425/1‑251 (MQ=255)
                        cAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCt                                                                                                                                                                                                         <  1:42562/251‑1 (MQ=255)
                             aaCGTTAACGATAACGGACTTCCTGATCCTGCAGCCCATGGAATTTATCTGGTAACGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGATTACGGCCACCGACATCCTTCAAc                                                                                                                                                                                                    >  2:370459/1‑251 (MQ=255)
                                    aCGATAACGGACTTCCTGATCCTGCAGACCATTGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCTCCCAGATCCCATTTTTTAAACTGATCGTTATAAACGCCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTAc                                                                                                                                                                                               <  2:208745/249‑1 (MQ=255)
                                    aCGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGCCGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTAc                                                                                                                                                                                               >  1:208745/1‑249 (MQ=255)
                                                     gATCCTGCAGGACATGGAATTTATCTGGTAAAGGGCGTAGTGCTTTAGGCAGCAGGCGCAGCGCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATg                                                                                                                                                                            <  2:99090/251‑1 (MQ=255)
                                                     gATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCAt                                                                                                                                                                             <  1:201804/250‑1 (MQ=255)
                                                         cTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac                                                                                                                                                                        >  2:69737/1‑251 (MQ=255)
                                                           gCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTGAGTTAGCAGGCGCAGCTCATTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAATCTGATCGTTATAAACCCCTGCTGTCAGGCTATCTCTTGCAGCGTGCATTTTGATTCGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTGCCCCATGATacga                                                                                                                                                                      <  2:387650/251‑1 (MQ=255)
                                                             aGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacgacg                                                                                                                                                                    >  1:77361/1‑251 (MQ=255)
                                                                 cATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc                                                                                                                                                                >  1:306209/1‑251 (MQ=255)
                                                                    ggAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATTTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcatc                                                                                                                                                             <  1:370459/251‑1 (MQ=255)
                                                                       aTTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCAtt                                                                                                                                                          <  1:242047/251‑1 (MQ=255)
                                                                              tGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCGGCTCAGTACAGAGAATGGAAAGATCACCCGTTTGCGTCTTAAACAGCGTAACGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTACACTGATCGTTCTACACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGCCATCATGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGcccg                                                                                                                                                   >  2:135083/1‑249 (MQ=255)
                                                                                                                    cAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTg                                                                                                              <  1:274147/250‑1 (MQ=255)
                                                                                                                           gTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCt                                                                                                      <  1:105258/251‑1 (MQ=255)
                                                                                                                                  gAAAGCTCACCCGTTTGCGCCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCGTCTTGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTGTAATGTTTAAGGAGTCCAGGTCCGGGTTATCCTTCGCATc                                                                                               <  2:365235/251‑1 (MQ=255)
                                                                                                                                          aCCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGCCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCtt                                                                                       >  2:11046/1‑251 (MQ=255)
                                                                                                                                                   cGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCATTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACt                                                                              <  1:315582/251‑1 (MQ=255)
                                                                                                                                                               cGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTAt                                                                  <  1:35870/251‑1 (MQ=255)
                                                                                                                                                                  aCCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGt                                                               <  1:69737/251‑1 (MQ=255)
                                                                                                                                                                   ccGCGGGCACCGATAATGGCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACCCCCTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCAGTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTc                                                              <  1:390647/251‑1 (MQ=255)
                                                                                                                                                                                               cccATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATACTGCAACGTTACCAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCcac                                   >  1:394468/1‑250 (MQ=255)
                                                                                                                                                                                                                tCGTTATAAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTgc                 >  1:345034/1‑251 (MQ=255)
                                                                                                                                                                                                                    tataAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTgccgcc             >  2:113062/1‑251 (MQ=255)
                                                                                                                                                                                                                     ataAACACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTgccgcc             >  1:308638/1‑250 (MQ=255)
                                                                                                                                                                                                                       aaaCACCTTCTGGCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTGCCGCCAGt          >  1:194832/1‑251 (MQ=255)
                                                                                                                                                                                                                                  ggCAGGCTATCTCGTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGCTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTGCCGCCAGTTTTTcgcg  <  1:5130/248‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
GGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCACCCAGATCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCATTGGGAAACGCCACACCTTGCTGACGTAGTGCCGCCAGTTTTTCGCG  >  NC_000913/4354178‑4354651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑