Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,344,4230TC74.3% 54.6 / 13.8 36I352T (ATC→ACC) melBmelibiose:H(+)/Na(+)/Li(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (2/7);  new base C (11/15);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCT  >  NC_000913/4344195‑4344665
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
gAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGTGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCTCA‑ACGTCGTCCGCATTGTGATTGCTGGTATTTTGCTGAATGTGGGAACGGCGCTTGTCTGGGTATGACATGTCATCATGGTTGCAGATATCGTTGATTACGGTGAATATaaa                                                                                                                                                                                                                              <  1:255204/251‑1 (MQ=255)
            tagcgACGTTGGTCTTCTTCCCACGCTTAGTTAAATCATTATCCCGCCGCATTTTATGGGCCGGAGCATCTATTCTAACGGTGTTAAGCTGTGTAGTTCTCCTGTTACTGACATTATTGAGCTAAC‑CACACGTCGTCCTCTTTGTGATTGCGGGTCTTTTGCTGAATGTGGGTACGGCGCTTTTCTGGGCATTACAGGTCATCATGGAGGCAGATACCGTTGATTACGGTAAATCGAACCTGCTCGTACGc                                                                                                                                                                                                                  >  2:6266/5‑251 (MQ=255)
                                                          aCGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCg                                                                                                                                                                    >  1:350021/1‑251 (MQ=255)
                                                            gCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGt                                                                                                                                                                  <  1:57330/251‑1 (MQ=255)
                                                              aTTTTATGGGCCGGAGCAACTATTCCTCCGGAGATAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTCTCAAA‑ACGTCGTCCTCCTTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCAGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGCAGGGCGGTTc                                                                                                                                                                >  2:272843/1‑251 (MQ=255)
                                                                tttATGGGCCGGTGCAGCTATTCTGCCGGTGTTAAGCTGTGGTGTTCGCCGGGTAATGGCATTAATGAGCGAGCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGTATGTGGGAACGGCGCTTTTTTGGGTATTACAGGTCATCATGGTGGCAGATTCCGTGGGTTACGGTGAATATAAACTGCACGTACGCTGTGATAGTATCGCTTACTCCGTGCAGACTAGGGTGGTGAAGGTCGTTTCa                                                                                                                                                               <  2:60787/250‑1 (MQ=255)
                                                                   aTGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCt                                                                                                                                                           <  1:178558/251‑1 (MQ=255)
                                                                      ggCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCGGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTg                                                                                                                                                        <  2:95904/251‑1 (MQ=255)
                                                                       gCCGGAGCCTCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGGCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATAGCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCATTTGc                                                                                                                                                       <  2:356651/251‑1 (MQ=255)
                                                                             gCATCTATTCTTCCGGTGTTAAGCTGTTGTTTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTTCTCCGTGCAGATTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCttt                                                                                                                                                 <  1:418476/251‑1 (MQ=255)
                                                                                   aTTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGGCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCtttttt                                                                                                                                              <  1:414711/248‑1 (MQ=255)
                                                                                              gtTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTc                                                                                                                                             <  2:298089/238‑1 (MQ=255)
                                                                                              gtTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTc                                                                                                                                             >  1:298089/1‑238 (MQ=255)
                                                                                                           gtTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTgg                                                                                                                   <  1:193646/251‑1 (MQ=255)
                                                                                                               tcCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTAtggtgg                                                                                                                                                                             >  1:218579/1‑189 (MQ=255)
                                                                                                               tcCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTAtggtgg                                                                                                                                                                             <  2:218579/189‑1 (MQ=255)
                                                                                                                         ggCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACATGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTTCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTTCGGCTTTTGTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATg                                                                                                     <  1:376829/251‑1 (MQ=255)
                                                                                                                                aTGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACa                                                                                              <  1:139202/251‑1 (MQ=255)
                                                                                                                                              aCGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGTTATTACAGGTCATCATGGTGGCAGATTCCGTTGATTCCGGTGAATATAAACTGCCCGTTCGCTGTGAAAGTATCGCTTACACCGTTCAGACTTTGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGcc                                                                                 <  1:71503/251‑1 (MQ=255)
                                                                                                                                               cgtcgtCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGccc                                                                                <  2:282908/251‑1 (MQ=255)
                                                                                                                                                  cgtcCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTAt                                                                             >  1:189507/1‑251 (MQ=255)
                                                                                                                                                                        aTTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTTAACAGGCTCCGCAAGCCCTATTAGGTATGCAGTTTATTATGAt                                                       >  2:340146/1‑251 (MQ=255)
                                                                                                                                                                          ttggCTGAATGTGGGTACGGCGCTTTTCTGGTTATTACAAGACATTATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTAAGCTGTGAAAGTATCGCGTGCTCCGGGCAGACTATGTGGGGGAAGGGCGGTTGTGCCTTTGAGTTTTGTTTCATTGCTGTTTTGTTATAGATGATGGTCTATGTGCCGTTTGTTGGTCAGTCTACGCTAGCATTATTAGTTTTGCAGgtgattgtgatt                                                      <  2:376199/247‑1 (MQ=255)
                                                                                                                                                                                    gtgGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGGATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTTCCGAATGGTTAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAAc                                           <  1:274220/251‑1 (MQ=255)
                                                                                                                                                                                            ggCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGtttt                                   >  2:140894/1‑251 (MQ=255)
                                                                                                                                                                                              cgcTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGAGTTCGGTGTATATAAACTGCACGGTCGCAGGGTAAGTTTCGCTTACTCCTTGCAGTGTATGGTGGTTAAGGGTGGTTCAGCCTTTGCGGTTTTTTTCATTGCGGGTGTGTTAGTGATGATTGGCTATGTGCCGAATGTTGAACAGTCGTCGCAAGCCCTATTAGGTATTCAGTTTATTATGATTGCTCTCCCAACTCTGTTTTTc                                 <  2:135262/251‑1 (MQ=255)
                                                                                                                                                                                               gcTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTtgt                                                                                                                                  <  2:15086/153‑1 (MQ=255)
                                                                                                                                                                                               gcTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTtgt                                                                                                                                  >  1:15086/1‑153 (MQ=255)
                                                                                                                                                                                                  tttCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTATGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCAt                               <  1:205941/249‑1 (MQ=255)
                                                                                                                                                                                                   ttCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGGCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATggg                            >  2:70684/1‑250 (MQ=255)
                                                                                                                                                                                                    tCTGGGTATTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCAGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTAAGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGt                            >  2:340679/1‑250 (MQ=255)
                                                                                                                                                                                                           aTTACAGGTCATCATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTg                     >  1:30372/1‑250 (MQ=255)
                                                                                                                                                                                                              acagggtatcatGGTGGCAGATACCGTTGAGTACGGTGAATATAAACGTCACGTACTCTGTTAAAGTATCGCTTACGCTGTGCTTACTATGTTGGTGATGGGCTGTTCAGCCTTTGCGGCGTTTTTCATGGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAAGGGTTATCAGTCTACGCAAGCCTTATTAGTTATGCCGTTTATTAGTATTGCTCTACCAATTCTGTTTTTCAAGGTAACGCTGAtt                  <  2:403671/243‑1 (MQ=255)
                                                                                                                                                                                                                        atGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTGCCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCg       >  1:196228/1‑251 (MQ=255)
                                                                                                                                                                                                                           gtggCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGttt    <  1:139/251‑1 (MQ=255)
                                                                                                                                                                                                                             ggCAGATATCGTTGATTACGGGGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTTAAGGGCGGTTCAGCTTTTGCGGCTTTTTTCTTTGCGGTTTTGTTAGGGATGATTGGCTATGTACCTAATGTTGAACAGTCTACGCAAGCCCTATTATGTATGCAGTTTATTAGGATTGCTCTACCAACTCTGGTTTTCATGGTAACTCTGATTCTCAACTTCCGTTTCt  <  2:338951/251‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
GAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACA‑ACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCT  >  NC_000913/4344195‑4344665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑