Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	371,758	IS30 (+) +2 bp	coding (520‑521/867 nt)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	270541 =	NA (NA)	28 (0.770)	28/482	0.3	100%	noncoding (1/1221 nt)	IS30	repeat region
	?	NC_000913	= 371759	0 (0.000)					coding (521/867 nt)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
*	?	NC_000913	371758 =	0 (0.000)	20 (0.550)	18/482	0.7	100%	coding (520/867 nt)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
	?	NC_000913	= 1470516	NA (NA)					noncoding (1221/1221 nt)	IS30	repeat region

GCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTGTCT  >  NC_000913/270297‑270788                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        gcgcAGAGTTTAAACGCGAATCCGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTTAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGAt                                                                                                                                                                                                                                                   <  1:268949/251‑1 (MQ=255)       agTTTAAACGCGAATCCGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGAATGTCGGCCTTTCCACAATGACGCGATGGGTGAAAAAATTACGTGATGAACGCCAGGGCAACACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAAtt                                                                                                                                                                                                                                             >  1:16990/1‑251 (MQ=255)          ttAAACGCGAATCCGCTAAACTGGTCGTTGAACAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACAAAAAGACTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGAAGATTCAATTGGt                                                                                                                                                                                                                                          >  2:53405/1‑251 (MQ=255)                      ccGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACACATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGTTCAACGCAACAGt                                                                                                                                                                                                                              >  2:330836/1‑251 (MQ=255)                                     ttGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGTAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGTAAAATGAAATATTAAAAAAGGCTACTGTAGATGCAATTGGTCAACGCAACAGTTATGTTAAAACATg                                                                                                                                                                                                                <  2:248084/250‑1 (MQ=255)                                       gACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTCTGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAACACACCAAAAGCCTCCCCCCTTACCCCGGAACAAATTGAAATCCGTGATCTCCGGAAAAAGCTCCAACGTATTGAAATGGAACATGAACTATTACAAAAGGCTACTGTAGATTCCATTGGTCAACGCCACAGTTATGTGAAACCATgggg                                                                                                                                                                                                             >  2:355597/1‑251 (MQ=255)                                                             aTGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATgag                                                                                                                                                                                       <  1:53405/251‑1 (MQ=255)                                                                                           ccACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCctc                                                                                                                                                         <  1:337654/251‑1 (MQ=255)                                                                                                              gAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATTATACGAACATTTACAGCTGAGGAAAAAGCCTCTGTTTTTGAACTATGgaa                                                                                                                                       <  1:190702/250‑1 (MQ=255)                                                                                                                                                     aGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGt                                                                                               >  1:68942/1‑251 (MQ=255)                                                                                                                                                                     ccGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCCACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGACCGGCACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACg                                                                                >  1:330888/1‑250 (MQ=255)                                                                                                                                                                      cGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGAt                                                                              >  1:155778/1‑251 (MQ=255)                                                                                                                                                                                      ccGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGTCTACTGTAGATTCAATTTGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAg                                                               <  2:306198/250‑1 (MQ=255)                                                                                                                                                                                          gAGCTCAGGTAAAATCTTCAACGTAGTTAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTGTATTGGGCAACGCATCAGGTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGTGACGAAAATTTACAGCAGAGGAAAAAGCCTCTGTTGTTGATCTATGGAAGAACGGAACAGGCTTCAGTGAGATAGCGAATATCCTGGGGTCAAAACCCGGAACGAGCTTCACTATGTTAAGGGAt                                                           <  2:333160/250‑1 (MQ=14)                                                                                                                                                                                           ggattaGGAAAAAGATACAACGGATTGAGAGGGAAAATGAAATATTAAAAAAGGATACTGTAGATTCAATTGGTCAACGCAACAGTTAGGTGAAAACATGGGGTTGCGGAGGTTGGTTGAAGGAGACGAACATTTACAGCAGAGTAAAAAGCCTCTGTTTGTGAGCTAGGGAAGAACGGAACATGCTTCAGTTAAATAGCTGATATCCTGGGTTCAATACCCGGAACGATCTTCACGATGTTAAGGGATa                                                          <  2:218373/245‑1 (MQ=14)                                                                                                                                                                                               cAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTg                                                       <  1:330836/249‑1 (MQ=255)                                                                                                                                                                                                         cTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTAATGTAGCTTCAATTGGTCAACGCAACAGTTATGAGAAAACATAGAGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAAc                                           >  2:147035/1‑251 (MQ=255)                                                                                                                                                                                                            cAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGtt                                                                                                                                                     <  2:219825/142‑1 (MQ=255)                                                                                                                                                                                                            cAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGtt                                                                                                                                                     >  1:219825/1‑142 (MQ=255)                                                                                                                                                                                                                             aaaTGATATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAgg                                                                                                                           >  1:110072/1‑151 (MQ=25)                                                                                                                                                                                                                             aaaTGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAgg                                                                                                                           <  2:110072/151‑1 (MQ=40)                                                                                                                                                                                                                                   aaTATTAAAAAAGGCTACTGTAGATTCAATTGGTCACCGCAACAGTTATGTGAAAACATGGGATTGCGGCGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCATCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGAGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAGGGGATACTGGCGGCATAAAACCCACTGAGCGTAAGAGGGCTGTAGcc                 >  2:277671/1‑250 (MQ=11)                                                                                                                                                                                                                                    atatTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCGCTGGTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATAGCCTGGTTTCAAAACCCGGAACGAGCTTGACTATGTTAAGGGATACTGGCGGCAAAAAACCCCATGAGCGTAAGCGGGCTGTAGCTc                <  1:364928/251‑1 (MQ=11)                                                                                                                                                                                                                                         aaaaaaGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAc                                                                                        >  1:410723/1‑174 (MQ=34)                                                                                                                                                                                                                                         aaaaaaGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAc                                                                                        <  2:410723/174‑1 (MQ=34)                                                                                                                                                                                                                                                  tACTGTAGATTCGATTGGTCAACGCATCAGATATATGAAAAAATGGTATTGCGGAGGTTTTTTGAATGAGACGAACATTTACAACAGAGGCAAAAGCCTCTGTTTTTGAAATATGGAAGATCGTAACAGGCTTCAGTGAAATAGCGAATAACCTGGGATCAAAACTCGGAACGATCTTCACTATGTTAAGGGATACTGACGGCATAAAACGCCATGCGCGTCAGCGGGCTGTACATCACCTGATAATGTCt  >  2:33178/1‑251 (MQ=11)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        GCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCAGCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGATGAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGTGAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTGTCT  >  NC_000913/270297‑270788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.