| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,380 | 1 | . | C | 100.0% | 81.0 / NA | 28 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (12/16); total (12/16) | |||||||||||
| * | NC_000913 | 4,296,380 | 2 | . | G | 92.9% | 72.4 / ‑6.1 | 28 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (12/14); minor base T (0/2); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTG > NC_000913/4296194‑4296613 || cgTCTTATCAGGCCTACGCCATACCGCGCAATGGCCTGATTTAGCGTGATTTTGTATGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCAAAAGCCTGATTTAGCTTGATTTTGTAGGACGGATAATGTGTTTACGCCGCATCCTATGTCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCg < 1:180505/251‑1 (MQ=11) tatggcatACGCCAGACAGCGCAATAGCCTAATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGtt < 2:125463/243‑1 (MQ=255) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct > 1:55389/1‑251 (MQ=255) cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc < 1:66232/251‑1 (MQ=255) aGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt < 1:236102/251‑1 (MQ=255) tgtttttGTAGGTCGAATAAGGCGTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTATGTCGGTCGGATAAGGCGTTTACGCCGCATCCAACATCAATGCCTGATGCAACGATTGC‑CGCATCTTATCCGGCCTATCTTACCCGTTGGCTAATATTTCAAACTCTTCACACCCGGTATCCAACCCTTcc > 2:16317/4‑250 (MQ=14) tAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCACTAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACTGCCTGCTGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc > 2:184318/1‑251 (MQ=17) gCGTTTATGCCGCATCCAACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGt < 2:329329/251‑1 (MQ=255) ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt > 1:12677/1‑251 (MQ=255) cgccATCAACGCCTGATGCGACGCTTAACGCGACTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGAGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAACCCCTTCCATACAGCTCAGCTTCACCCAGTGCAGCGCCTTTTgact > 2:331830/4‑248 (MQ=18) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTtctc < 1:184318/251‑1 (MQ=255) ccgtATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGATCTGGATTTTGTAGGTCTGATTCGTCGTTTACTCCGCATCCGACATCAATGCCTGATTCTTCGCTTTC‑CGCGTCTTATCAGGCCTATCTTAACCGTTTGTTAATTTTTCAATCTCTTCTCACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCATCGCCTTTTGCTTGTTCTTCTCCATAAAGCCTGCTTCACCGTTTa < 2:158955/248‑1 (MQ=21) tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTa > 1:47946/1‑248 (MQ=255) acAGGCCTACTCCAGACAGCGCAATACTATGTTATTGGGTGAGATTGGAGGTCAGATAATGCGTTTACTCCTCATCCGCCATCAAAGCCTGAGTCGACG‑ATGCGCGAGTCTTATCAGGCCTCTCTTAACCGTTGTTCAATTTTTCAAACTCTTCACACCCGGTATCAATCTCTTCCATCCAGCTCAGGTTCAACCAGTGAAGCTACGTATGCTTGTTCTGCTCGTTTAAACCTTCTTCACCGTTTAAGAAc < 2:220816/250‑1 (MQ=255) cAGGCATACGCCAGACAGCGCAATAGCATGATTTAGCGTTATTTTTTAGGTCGGATAAGGCGTTTACGCCGCATCCTACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCATCCATTGCAGCGCCTTTGGCTTGTTCTGCGCTATAAATCCTTCTTCACCGTTTAAGAAc < 2:50463/250‑1 (MQ=21) ccTACGCCAGACAGCGCAATAGCATGATTTAGTGTGATTTTGTGGGTCGGATAACGCGTTTACGCCGCCTCCGACATCAATGCCTGATGCGATGCTTTC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAACCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCAGTGCAGCGCCTTTTTCTTGTTCTTCGCGATATAACCTTCTTCTCCGTTTAAGACcatcat < 2:325376/251‑1 (MQ=255) cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt > 1:36233/1‑251 (MQ=255) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt > 1:400765/1‑250 (MQ=255) gcgcAATAGCCTGATTAAGCGTGATTTAGTAGGGCGGATAAAGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc < 2:422937/251‑1 (MQ=38) cgcAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGTCATCACTGCCTGATGCGACGCTTGC‑CGCGTCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt > 2:420953/1‑144 (MQ=18) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt < 1:420953/144‑1 (MQ=255) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGtt > 2:194432/1‑224 (MQ=255) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGtt < 1:194432/224‑1 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTACGCACATCATTCCCGCCCAGTACTc > 2:61919/1‑241 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc < 1:61919/241‑1 (MQ=255) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAAtt < 1:224557/250‑1 (MQ=255) tCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAAGCTTGC‑CGCGTCTTATCAGGCATCTCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTg > 1:403620/1‑251 (MQ=255) gcGGATAAGGCGTTTACGCCGCATCCGACATCAAGGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGGTCGGGAAATTGCAGAGCTg < 2:152937/250‑1 (MQ=255) || CGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGC‑CGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTG > NC_000913/4296194‑4296613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |