| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,311,002 | 2 bp→GT | intergenic (‑22/+104) | alsA ← / ← alsB | D‑allose ABC transporter ATP binding subunit/D‑allose ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,311,002 | 0 | T | G | 96.8% | 87.8 / ‑7.3 | 31 | intergenic (‑22/+105) | alsA/alsB | D‑allose ABC transporter ATP binding subunit/D‑allose ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (20/10); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,311,003 | 0 | A | T | 100.0% | 99.1 / NA | 31 | intergenic (‑23/+104) | alsA/alsB | D‑allose ABC transporter ATP binding subunit/D‑allose ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (21/10); total (21/10) | |||||||||||
AATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACC > NC_000913/4310780‑4311242 || aaTGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACAt < 1:148451/249‑1 (MQ=255) atggtactggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTACCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGCAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCt > 2:319569/1‑250 (MQ=255) cTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTCGTAATGCCTGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTAAGGTTATTTTGTTGCCCGGATAAGACACTTCATTAGc > 1:326474/1‑250 (MQ=255) cGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCATGTCTACGGTCAGGTTATTTTGTTGGCCGGATAAGTCACTTCATTAGCATCATCATCCg < 2:152949/251‑1 (MQ=255) aTTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcatc > 1:171288/1‑251 (MQ=255) aCCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCAtt < 1:321904/250‑1 (MQ=255) cccGCGCCATTTTCTCCTAGTAATGCATGTATTTCTCCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCTATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCAACATTCa < 1:93650/251‑1 (MQ=255) cATTTTATCCTAGTCAGGCATGTATTTCACCACGATAAACCGATAACTTAACCGACTTTAATGAGTGAACCGGACCAAAGGACTTGCCGATTCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAGATTACGTCTTATTGACTGATGCCTACATTCTTCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatcacacgggctacttcagttttgt > 2:139175/1‑230 (MQ=255) tttCTCCTAGTAAAGCATGTATTTCACCAGGATAAACCGTTAACTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTgt > 2:359212/1‑250 (MQ=255) aTGCATGTATTTCACCAGGATAAACCGTTAAATGAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAtt < 1:107857/251‑1 (MQ=255) gTATTTCACAAGGATCAACCGTTAAATTACCGGCCTTTAATACGTGAACCGGACCAAAGAACATGCCGAACCCCGCCAACGATCTCTATGGCGCCACCATAAATAACCTCTCTGCACGACAGTTCGTATTATTCACTGATGGCTACATTTATCAGATCTACGTTCAGGTTATTTTGTTGGACGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTAACCAGGATTGAATCg > 2:51931/1‑251 (MQ=255) aCCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGt < 1:220068/218‑1 (MQ=255) aCCAGGATAAACCGTTAAATTAAACGACTTTAATGAGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGt > 2:220068/1‑218 (MQ=255) ggATAAACCGTTAAATTAACCGAATTTAATGCGTGAACCGGACCAAAGGACTTGCCGATACCCACCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATTAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGGATCATCATCCGACATCATTCAGTTTTATTGAGTGACCATGATTGAATCGCCCAGTTTaa > 2:333913/1‑250 (MQ=255) aTAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGACAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCCTCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAAtt > 2:326842/1‑251 (MQ=255) aCCGTTAAATTCACCGACTTTACTGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCTATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGCAAGTTCGTATTATTGCCTGATGGCTAAATTTATCAGGTCTACGATCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCCGGATTGAATCGACCAGGTTAAATTCCgg > 2:46746/1‑251 (MQ=255) aCCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATcc > 1:301804/1‑249 (MQ=255) ttAATGCGTGCACCGAACCACAGGACTTGCCGATCCCCGCCATCGAAATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTAAGTTCAAGTTATTTTGTTGGCCGGATAAGACCATTCATTAGCATCATCATCCGGCCTCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGAt > 2:137447/1‑250 (MQ=255) tAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCAATCGCTATATATGACGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATa > 1:23462/1‑250 (MQ=255) aaTGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAAc > 1:137501/1‑251 (MQ=255) ggtccAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGAGATCCTTGCCGGAttt < 2:301804/248‑1 (MQ=255) aCCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTtct > 1:305595/1‑251 (MQ=255) cAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTtctc > 2:216093/1‑250 (MQ=255) aTCCCCGCCATCGATATATATAGCATGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGCCACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATACCCTTGCCGGTTTTCTCAGCGTCAACCATCac > 2:24332/1‑250 (MQ=255) aTCCCCGCCATAGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGCCCAGGATTGAATCGACCAGTTTACATTCCGGTGCTTTATACAGCGGGGTTACCTTGCCGGATTTCTCAGCGTCAACCATc > 2:130825/1‑249 (MQ=255) aTCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTTCGTTCAGGTTATTTTGTTGGCCGGCTAAGACACTTCGTTAACATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAAACTTGCAGGATTTCTCAGCGTCAACCATCAGCTTCAGAcc > 1:118553/1‑251 (MQ=255) ataaCCTCTCTGCACGAAAGTTCGTATCATTGCATGATGGCAACATTTATCAGGTATACGTTCAGGTTATTTTGTTGGCCGGATACGACACTTCATTAGCATCATCATCCGTCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGCCCAGTTTAAGTTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGttt > 2:16772/1‑250 (MQ=255) aCCTCTCTGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTgg < 1:183041/251‑1 (MQ=255) ctGCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCa < 1:216093/248‑1 (MQ=255) tGCACGAAAGTTCGTATTATTGCCTGATGGATACATTTATCAGGTATACGTTAAGGCTTTTTTGTAGGACGGATAAGACACTTCATTAGCATCACCCACCGGCAAAATTCAGTTTTATTGAGTGAACAGAATTGAATCGACCAGTTTAAATTACGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATCTCCGCCGGGTTCTGGGCAAc > 1:160971/1‑249 (MQ=255) gCACGAAAGTTCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAAcc < 2:171288/249‑1 (MQ=255) || AATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACC > NC_000913/4310780‑4311242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |