Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,556	G→A	S374S (AGC→AGT)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,556	0	G	A	97.6%	127.9 / NA	42	S374S (AGC→AGT)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/16);  minor base C (1/0);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTCGCGCCAGTCACTGCCGCGCGTGCCGGAGTAGAGCAGCGC  >  NC_000913/4515309‑4515798                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   gggCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTc                                                                                                                                                                                                                                                 <  2:349535/250‑1 (MQ=255)                           gTTGATTCATTCAAATAGCGATAGCCCACGCCCCCTTCGTGCGCGGAAGGGCCGATCATAAAGCTCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAcgcg                                                                                                                                                                                                                       >  2:143268/1‑251 (MQ=255)                                ttcattcaAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCAt                                                                                                                                                                                                                  >  1:66502/1‑251 (MQ=255)                                   attcacaaagacaTAGCCCACGCCCACTTAGAGCGCGGAAGGGCCGATCATAAAGATCTAGATATAGAGAGGCTCAATCCCGCGCACCCCGTAGCTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCCGGGATTGGGGGTAGAACCCCTGACTGTTGAATTTACGCTGGCTGTCTGGCTGGAACTGGTAGCCTAGCCTCGACCGCTTGCGACGACCACAAAAGAGGTCaaa                                                                                                                                                                                                                 >  2:232271/13‑247 (MQ=255)                                     tcaAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATAcgg                                                                                                                                                                                                              <  1:173153/250‑1 (MQ=255)                                        aaTAGCGATAGCCCCCGCCCACGTCGTGCGCGGAAGGTCCGATCATAAAGATCGGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTGACGTTGCGAGAGGGTG‑ATGCATTTGCCTTGCTCCAGGTCGCCCCGGCGCAGGGTTGGGATTTATAAGTCCTGAATTTTGAATTTATGCTGTCTGTCCGGCTGGAGCTGGTAGCCGAGATACGCCAGCTTGTGTCGACCCCAGAATCGGTCATAcgggcgg                                                                                                                                                                                                          <  2:170853/251‑1 (MQ=255)                                             cGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTgg                                                                                                                                                                                                      >  1:32864/1‑250 (MQ=255)                                                     cacgccctcTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCTGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTg                                                                                                                                                                                                       <  1:274610/241‑1 (MQ=255)                                                     cacgcccacTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCTGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATAcgggcgggag                                                                                                                                                                                                       >  2:274610/1‑239 (MQ=255)                                                           cacTTCGTGCGCGGAAGGGCCGATCATACAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGCTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCACAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGGTTGCCCGCGacagg                                                                                                                                                                                       >  2:280491/1‑247 (MQ=255)                                                                 gTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCCGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGGGGATTGCCAGCGAACGGcgtcgg                                                                                                                                                                                 >  2:308702/1‑250 (MQ=255)                                                                 gTGCGCGGAAGGGCCGATCATAAAGATCCGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGCG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGCGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGGTTGCCAGCGATCGGcgtcgt                                                                                                                                                                                 >  2:339366/1‑251 (MQ=255)                                                                                      aaaGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCAc                                                                                                                                                            >  1:45441/1‑251 (MQ=255)                                                                                      aaaGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGGTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCAc                                                                                                                                                            >  1:258261/1‑251 (MQ=255)                                                                                           tCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGACAGCGATCGGCGTAGTAATCCGCGCGAGACAGGCCACCGGGc                                                                                                                                                       >  1:92802/1‑251 (MQ=255)                                                                                                  ttAGCGTGGCGCAATACCGCGCACCCAGTAGTTACGCGGCGAG‑TGGTGTATGCGTTTTCCTTGCTCCAGTTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTTAATTTATGCTGGCTGTCTTGCTGGAACGGGTAGCCCAGACGCGCCAGCGTGCGACGACCCCAGAAGCGTTCATACGGGCGGTTGGATTGCCATCGATCGGCGTCTTAATCCGCGCGAGACAGGCCACCGGGCATGTCg                                                                                                                                                 <  1:177594/249‑1 (MQ=255)                                                                                                       gTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTCATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTc                                                                                                                                            >  1:137219/1‑250 (MQ=255)                                                                                                          gCTCAATACCTCGCTCCCAGTAGTTACGCGGCGAGGGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAAGTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCTCCGGGCATGTCGGCTTCACcg                                                                                                                                        <  2:129111/251‑1 (MQ=255)                                                                                                               aTACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGCAGCCGCTGCGCAGGGTTTGGGTGCAGCACCCCTGAATGTTGAATTTCTGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGGGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAACCAGGCCCCAGGGCCTGTCGGCTTCACcgtcgt                                                                                                                                    >  2:339164/1‑250 (MQ=255)                                                                                                                   cgcgCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGGTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGGCGGCTTCACCGTCGTAatat                                                                                                                               >  2:195923/1‑251 (MQ=255)                                                                                                                     cgcACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTg                                                                                                                             >  1:165214/1‑251 (MQ=255)                                                                                                                       cACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTgc                                                                                                                            >  1:89171/1‑250 (MQ=255)                                                                                                                        aCCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTgc                                                                                                                            >  1:289667/1‑249 (MQ=255)                                                                                                                                     gcggcgAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGACGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGa                                                                                                               >  2:334091/1‑249 (MQ=255)                                                                                                                                        gcgAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGgtg                                                                                                          >  1:154626/1‑251 (MQ=255)                                                                                                                                          gagaGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTCATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGtgtg                                                                                                        <  2:137219/251‑1 (MQ=255)                                                                                                                                                gTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTc                                                                                                  <  1:49264/251‑1 (MQ=255)                                                                                                                                                      gCGTTTTCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAAACCCTGAATGTGGAATTTATGCTGGCTGGCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCg                                                                                             <  2:199807/251‑1 (MQ=255)                                                                                                                                                                     cAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTTCGACGACCCCAGAAGCGGTCATACGGGCGGGTTGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCTGCTTCACCGTCTTAATAGTGCAGCAGGCGGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCttt                                                                              <  1:151463/251‑1 (MQ=255)                                                                                                                                                                         tAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCAACGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCCGTTGGAGGGGTGACCCTCATCCGGCGCACATTTGCTTTTCa                                                                           >  2:295640/1‑250 (MQ=255)                                                                                                                                                                                tGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGAACCCAGCAGCGGACATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGACCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTTCTTTTCAGCATCAgg                                                                   >  1:169128/1‑251 (MQ=255)                                                                                                                                                                                           tGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGc                                                         <  1:341908/250‑1 (MQ=255)                                                                                                                                                                                                 tAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCCCCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTAGATGCGGGTggg                                                  >  2:42148/1‑250 (MQ=255)                                                                                                                                                                                                  aGAACCCCTGAATGTTGAATTTATGCTGGCTGTCGGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCAGCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGgc                                                  <  2:222202/250‑1 (MQ=255)                                                                                                                                                                                                     aCCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCtg                                              >  1:226734/1‑251 (MQ=255)                                                                                                                                                                                                        ccTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGc                                           <  1:23540/251‑1 (MQ=255)                                                                                                                                                                                                           gAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCt                                          <  1:320804/249‑1 (MQ=255)                                                                                                                                                                                                           gAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTc                                         <  1:334091/250‑1 (MQ=255)                                                                                                                                                                                                                             ggctgTCTGGCTGGAACTGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTCGCGCCAGTCACTGCcgc                        <  1:32984/249‑1 (MQ=255)                                                                                                                                                                                                                                              tGGTAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGGGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTCGCGCCAGTCACTGCCGCGCGTGCCGGAGTAGagc       >  1:209453/1‑249 (MQ=255)                                                                                                                                                                                                                                                 tAGCCCAGACTCGCCAGCTTGCGCCGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGACAGCGATCGGCGTCGTAATCCGCGCGCGACAGGCCACCGGGCCTGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGGGCACCTCCTCCGGCGCATGTTTGCTTTTCAGCCTCAGGTCGTCGATGCGGGGGGCGCTGAGCTCGCGCCAGTCACTGCCGCGCGTGCCGGCGGAGAGCAgcgc  >  2:141036/1‑251 (MQ=255)                                                                                                                                                                                                                                                 tAGCCCAGACTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTCGCGCCAGTCACTGCCGCGCGTGCCGGAGTAGAGCAgcgc  >  1:190951/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   TGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTG‑ATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATGCGGGTGGCGCTGTGCTCGCGCCAGTCACTGCCGCGCGTGCCGGAGTAGAGCAGCGC  >  NC_000913/4515309‑4515798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑