Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,392,517 G→A noncoding (46/76 nt) glyX → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,5170GA96.2% 56.3 / NA 26noncoding (46/76 nt)glyXtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/11);  minor base C (0/1);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCT  >  NC_000913/4392279‑4392756
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
agagGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCg                                                                                                                                                                                                                                      >  1:105434/1‑250 (MQ=255)
      aaaGATTTCTCGTATAATGCGCCTACCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTACCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCg                                                                                                                                                                                                                               <  2:99153/251‑1 (MQ=255)
             tctcGTGTAATGGGCCGCTCGTAACGACGCAGAAATGCGAAAAGTGCGAAAGCAAAATTTAGTAGTACGCGGGAAAAGCACAGTTGGTAGAGCACGACCTTGCGAAGGGCGGGGTCGCGAGATCGAGTCTCCGTTCCGGCTCCAAAATGTGAAAAGTGCTGCAGAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTccc                                                                                                                                                                                                                         <  2:276712/250‑1 (MQ=17)
               tcGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCt                                                                                                                                                                                                                      >  1:131562/1‑251 (MQ=255)
                      aTGCTCCTCCCGTAAAGACACAGAAATGCGAAAATTACGAACGCAAACTTAAGTAGTACGCGGGCATAGCTCAGTTGGTAGAGCACGACCTTGCCACGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCCAGCGGGAATAGCTCCGTTGGTAAAACACTACCTTGCACTGGACGGGATC‑GCGAGTTCGCGCCTCGTTCCCCGATCCaactt                                                                                                                                                                                                               >  2:365538/1‑248 (MQ=17)
                                   aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTg                                                                                                                                                                                                   <  1:22883/250‑1 (MQ=255)
                                         gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGTTGAAAAGCCCAGACCACCCAACCGGGAATAGCCCAGTTGGGAGAGCGCGTCCATGCCCGGGTCGGGAGC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAACATTTGCAAGGGCTGTacagcc                                                                                                                                                                                            >  2:169089/1‑246 (MQ=17)
                                                  cGAAAATTAAGAAAGTAAAATTAAGAAGTACGAGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGGCTGGGTCGCGAGTTCGAGTCGCGTTACCCGCGCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccacc                                                                                                                                                                                   <  2:104868/251‑1 (MQ=18)
                                                   gAAAATCACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                                                                                                                                                                                  >  1:96787/1‑251 (MQ=255)
                                                                           tagtaCGCGAGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGAGAATTCTAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGAGCTGCAAATCTCAGACCACCCAAGCGAAAATAGCTCAATAGGTAGAGCACGACCTTGCAAAGGACGGGATC‑ACGAGTACGAGTCTCAGTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAATCGGGAATAGATCAGtttgt                                                                                                                                                            >  1:56721/1‑246 (MQ=18)
                                                                             gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGAAAAGCCCAGACCACCCACGCGGGAATAGCTCAGTTGGTAGATCACGACCTTGCCAAGGTCGGGATC‑GGTATTTCGAGTCTCGTTTCCTGCTCCAAATTTTGAAAGAACTTGAAGCGACAGACCACCCAATTTGGAATAGCTCattgttgag                                                                                                                                                          >  2:272769/1‑241 (MQ=11)
                                                                                      aaTAGCTCATTTTGTAGAGCACGACCGTGCCAAGGTGGGTGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAAGTACTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTTGTAGAGCACGACCtt                                                                                                                                               <  1:45870/251‑1 (MQ=18)
                                                                                        tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCACTTGGTAGAGCCCGACCTTGc                                                                                                                                             >  1:136396/1‑251 (MQ=255)
                                                                                              aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAAATTTGAAAAGAGATGCAAAGCACGGCCCACCCAAGCGGGAATAGCTCAGTTGGTAGGGCCCGCCCTTGCAAAGGTCGGGATC‑GCGAGTTCGAGTCGCGTTTCCCGCTCCAAAATTTGAACGTGCTTTCAGGCACCGACCACCCAAGCGGGGATAGCTCAGTTGGTAGGGCACGACCTTGCCa                                                                                                                                           >  2:213774/1‑247 (MQ=18)
                                                                                                gtGGTAGAGCACGTCCGTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                                                     <  1:34476/250‑1 (MQ=255)
                                                                                                                         gcggggcctccacatagaccctcGTTTCCCGCTCCAAAAATTGAAAAGTGTAGCAAAGCACAGACCACCCAAGCGGGAATAGCCCAGTGGGTATAGCACGACCGTCCCACGG‑CGAGATCTACGAGTTCGATCCTCGTTTCCCGCTCCAAAATTTAAAAGTGCTGTAAGGCACAGACGAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                                            <  2:96787/231‑1 (MQ=12)
                                                                                                                                             ctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                                                                        >  1:76433/1‑251 (MQ=255)
                                                                                                                                               cGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGGCGGGATC‑GCGAGTTCGAGTCTCGTTCCCCGCTCCAAAAGTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                                                                        <  1:213774/249‑1 (MQ=255)
                                                                                                                                                 tttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCCCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGCTCGGGATC‑ACGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccac                                                                                                                                                                                    >  1:224794/1‑155 (MQ=18)
                                                                                                                                                 tttCCCGCTCCAAAATTGGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGTAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGTTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGGCTGTAAGGCACAGaccac                                                                                                                                                                                    <  2:224794/155‑1 (MQ=18)
                                                                                                                                                  tccccGCGCCAAAAATTTAAAAGTGCTGCACAGCCCAGACCGCCCAAGCGGGAATAGCCCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGCTC‑GCGAGTCCGATTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATa                                                                                    <  2:136396/248‑1 (MQ=17)
                                                                                                                                                                  tGAAAAGTGCTGAAAAGCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCCCGACCTTGCCAAGGTCGGGATC‑GCGAGTGCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAc                                                                    <  1:169089/250‑1 (MQ=255)
                                                                                                                                                                          gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGt                                                           >  1:47317/1‑251 (MQ=255)
                                                                                                                                                                                      aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGCGCACGACCTTGCCAAGGCCGGCATG‑GCGAGTTAGAGTCTCGTTTCCCACTCCAACATATCCAAGTGCTGTAAGGAACAGAACACCCAAGCGGGAATAGCCCAGTTGTTAGAGAACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGACTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACATCGACGCGATGCGTTATTGCTGGttt                                               >  1:237095/1‑251 (MQ=18)
                                                                                                                                                                                                                               cTTGCCAAGGTCGGGATC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGCGCTGTAAGGCACAGACCACCAAAGCGGGAATAGCTCAGTTGGTAGAGCAAGACCTTGCCAAGGTCGGGGTCGCGCGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACTGAGTTATCcac       >  1:225159/1‑250 (MQ=255)
                                                                                                                                                                                                                                   ccAAGGGCGGGATC‑GCGAGTTCGAGTCTCGCTTCCCGCGCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTGTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCt  <  2:47317/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
AGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTC‑GCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCT  >  NC_000913/4392279‑4392756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑