Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,180	G→A	intergenic (‑2/+50)	rarD ← / ← yigI	putative transporter RarD/putative thioesterase YigI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,180	0	G	A	100.0%	79.0 / NA	25	intergenic (‑2/+50)	rarD/yigI	putative transporter RarD/putative thioesterase YigI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

CAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCC  >  NC_000913/4003951‑4004414                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           cAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAAACAACTTTGAGTACGCCGGCGCTATACCCCAAATAAAAAAAA‑CGGCACGAGCCGGAAAAACGCCCCGCCCCGCTTGGTTTTCATCCCTCAGGAATACCCACTTTTattttg                                                                                                                                                                                                                       >  1:139024/1‑246 (MQ=255)                                  cGTTGTTAAATAGGACCACGGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGTCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGGCTTCAAc                                                                                                                                                                                      <  2:21143/251‑1 (MQ=255)                                   gTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGGAAGAAAACAAATTGAAGAACCCTGGCGCTATACCCCCAATAAAAAAAA‑CGGCAAGAGGGAGTAAAAAGCCCTGCCCCGTTTTTTTTTCATCCATCAGGAATAATCATTTTTTATTGGTAACAGAAGTTTTCCTGCTTTTTTGTTTTTAAcc                                                                                                                                                                                     >  2:295112/1‑251 (MQ=255)                                                   aCTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCCCGGAGGTAATAAAATTGAAGTACGCTGGGGCCATAACCCCAATAAAATAAAACGG‑AAGAGCGAGGAAAACGCCCCGCCCCGTTTGTTTTGCATCCATCAGGGATACTCATTTTTTATTTGGAAAAGCCGATTTCCTGCTTTTTTGTCTTTCACCTACCCTATAagtggcg                                                                                                                                                                     >  1:20560/1‑251 (MQ=255)                                                    cTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAAGCTGGCGCTTTAACCCAAAAAAAATAAA‑CGGCCAGAGCGGGGAATACGCCCCGCCGCGGTTGTTTTGCATCCATCAGGAATACTCATTTTTTATTGGTAACAGCAGTTTAACTGCTTTTATGTCTTCAACCTTCCATATAggtggcgg                                                                                                                                                                    >  1:162456/1‑251 (MQ=255)                                                           cAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGTCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACGCATTTTTAATTTGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACt                                                                                                                                                             <  1:117597/251‑1 (MQ=255)                                                            aaaTGCTCATCAGCACCTCCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTg                                                                                                                                                            <  2:241418/251‑1 (MQ=255)                                                                       aGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAg                                                                                                                                                 <  1:295112/251‑1 (MQ=255)                                                                            caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGtt                                                                                                                                            <  1:3239/251‑1 (MQ=255)                                                                                 aaaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACGTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAAGTGGTAACAGCAGTTTACCTGCTTTGATGTCTGCAACCTACCATATAGGTGGCGGTGGCACTTGCAATATAAAGCTGTTCTTCa                                                                                                                                       <  2:154305/250‑1 (MQ=255)                                                                                   aaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCAtt                                                                                                                                     <  1:234798/251‑1 (MQ=255)                                                                                    aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAAAAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATAATCATTTTTAATTGGTAACAGCAGGTTACCTGCTTTTTTTTCTTCAACCTACCATATAGGTGGCGGGGGCCCTGGCAATATAAAACTGTTCTTCATtg                                                                                                                                    >  1:133753/1‑251 (MQ=255)                                                                                                       gcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAAGGTGGGGGTATACCCCAAAAAAAAAAAA‑CGGCAAGAGAGGGGAAAACGGCCTCCCGCGGTTGTTTTGCCTCCCACAGGAAAACTAATTTTTTATTTGTAACAGCCGGTTAACACCTTTTATGTCTTCTAACTACCATATAGGAGGCGGTGGCACTGGCAACTTAAAACTGTTCTTCATTGTGTaaattaaccccggggg                                                                                                                 >  1:123528/1‑237 (MQ=255)                                                                                                            gCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGggcgacggc                                                                                                             <  2:123528/250‑1 (MQ=255)                                                                                                                             gcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCATGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCTGTGGCACTGGCAATATAAAGCTGTTCTTCATTTTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACg                                                                                           <  2:106902/251‑1 (MQ=255)                                                                                                                                 gCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAAc                                                                                       >  1:228759/1‑251 (MQ=255)                                                                                                                                     gtagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCa                                                                                         >  2:70828/1‑245 (MQ=255)                                                                                                                                     gtagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCa                                                                                         <  1:70828/245‑1 (MQ=255)                                                                                                                                                  ttGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGt                                                                      >  1:220738/1‑251 (MQ=255)                                                                                                                                                   tGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAg                                                                       <  1:251182/249‑1 (MQ=255)                                                                                                                                                   ggAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCGTCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTAGGTCTTCAACCTACCATATAGGTGGCTGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTAATTGCCTGCACTCAACAGGCGACTAGTAGCAg                                                                       <  2:223592/248‑1 (MQ=255)                                                                                                                                                         aCGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGc                                                               <  1:60734/251‑1 (MQ=255)                                                                                                                                                                                    aaG‑CGGCCAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATAATCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCa                                                                                             >  1:305014/1‑194 (MQ=255)                                                                                                                                                                                    aaG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATAGAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAAGTCAACGCGGGCGACGGCGACTTTATGGCCTGCa                                                                                             <  2:305014/194‑1 (MQ=255)                                                                                                                                                                                                                       ttgtttTGCATCCATCAGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGcc  >  1:141/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           CAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG‑CGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCC  >  NC_000913/4003951‑4004414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑