Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,633,668	T→A	intergenic (‑1078/‑1173)	yhiJ ← / → yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,633,668	0	T	A	100.0%	67.5 / NA	20	intergenic (‑1078/‑1173)	yhiJ/yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/11);  total (9/11)

TTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTT  >  NC_000913/3633452‑3633908                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 ttattaATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCtt                                                                                                                                                                                                                 <  1:291175/250‑1 (MQ=255)                        aCCCTCCAAATTTCTCATATAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGTAAGATACAAGGGGTTATCGCACCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAATATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGGATGCAGATGAATCCATCTCTTCGTGTTTTAATAACTCACataag                                                                                                                                                                                          <  2:56922/250‑2 (MQ=255)                                                                     aTCACCAAACATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCAAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGc                                                                                                                                             >  2:358657/1‑250 (MQ=255)                                                                      tCACCAAAACAAGAACTAAATCTATCGCATGTCTGATCCCCAAGGAAGTAACAAGGGGTTATCGCATCGCCCTTTAAAAAACCATGTTGACGCAACTAATTCAAGATCATGTCTCTCGAATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCAATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCACACCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCa                                                                                                                                            >  2:348153/1‑250 (MQ=255)                                                                         ccAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGc                                                                                                                                        <  2:347826/251‑1 (MQ=255)                                                                                    aCTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGGTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTGTAGTAATTTTATGTTTGTATGCAGATGTAGCCATCGCTTCGTGTTTTAATAATTCTCATAATAAACTGCAACCCATTTCCGTGATGCCTATATGCCCTGTAACGAGCAATGCGTAAAGTACa                                                                                                                              <  2:48965/250‑1 (MQ=255)                                                                                                       tGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGt                                                                                                          >  2:12476/1‑251 (MQ=255)                                                                                                           ccccAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCg                                                                                                      >  1:312213/1‑251 (MQ=255)                                                                                                                     gATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAg                                                                                            <  2:98850/251‑1 (MQ=255)                                                                                                                                 tATCGCATCGTCCTTTAAAAAAACATGTTGATTCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGAAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATCAGCCGTGTTTGCa                                                                                 <  2:184751/250‑1 (MQ=255)                                                                                                                                            ccTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCAGCTGTATCGCCAAAATAAGCCGTGTTGGTATTTTGTGGGtt                                                                      <  1:358657/250‑1 (MQ=255)                                                                                                                                                     aaaCATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATcacacca                                                            <  2:56455/251‑1 (MQ=255)                                                                                                                                                           gTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCAATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGt                                                      >  1:174540/1‑251 (MQ=255)                                                                                                                                                                         ttCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCGGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCt                                          <  2:155343/249‑1 (MQ=255)                                                                                                                                                                              gATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTg                                   >  1:43808/1‑251 (MQ=255)                                                                                                                                                                                             aTTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTaa                    <  2:312213/251‑1 (MQ=255)                                                                                                                                                                                                   taaATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGc              >  1:253070/1‑251 (MQ=255)                                                                                                                                                                                                         gTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATAACACCACACTGATTATAGAAGTCTATTTTTGAAAACACAGATTTAACATCGCTATCa         >  2:330247/1‑250 (MQ=255)                                                                                                                                                                                                             agCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACtt    <  1:356356/251‑1 (MQ=255)                                                                                                                                                                                                               cACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACtttt  >  1:220072/1‑251 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 TTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTT  >  NC_000913/3633452‑3633908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑