Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,497	G→A	Q232Q (CAG→CAA)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,497	0	G	A	97.7%	141.3 / NA	44	Q232Q (CAG→CAA)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (24/18);  minor base C (1/0);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCT  >  NC_000913/3065258‑3065720                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                cGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATaa                                                                                                                                                                                                                      <  1:8952/251‑1 (MQ=255) cGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGGGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATa                                                                                                                                                                                                                       >  2:213901/1‑250 (MQ=255)   ggCGAAGAAAGTGAGCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACg                                                                                                                                                                                                                    <  2:85715/251‑1 (MQ=255)           aaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACg                                                                                                                                                                                                            <  1:273994/251‑1 (MQ=255)           aaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTGGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACg                                                                                                                                                                                                            <  1:319061/251‑1 (MQ=255)                            aaTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATGCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAAt                                                                                                                                                                                           <  2:100977/251‑1 (MQ=255)                                     cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGAGCCCCGGCGGCGCAATAGCGTGTCGATCTACCATGCAATGGATCGCGTCGGGCCCCGCTATGTGCAAATCCATCCGAAAAAGATTGTCACCGACGAGGTAACCAACTTGCCCG‑ACGCCGGGAATATGCTGAATAAACAAAATCCCATGGGCCAGCGAATTGCGGGTAACGTTGTCACGTTCTTATTGCAGGAAACGGCGCATgg                                                                                                                                                                                  <  1:358572/251‑1 (MQ=255)                                      aCGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCTCTATGTGCAAATCGATCCGAAAAAGATTGTCACCGTCGTGGAAACCAAATTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAACAAATTGCCGATAAAGTTGTCACGTTCTTATTGCAGGAAATGGCGCATggg                                                                                                                                                                                 >  1:240206/1‑251 (MQ=255)                                      aCGATCCGCGCGTTGCAGAACTGGCGGATAATGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGAGCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTGATTGCAGGAAATGGCGCATggg                                                                                                                                                                                 <  2:133101/251‑1 (MQ=255)                                       cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGt                                                                                                                                                                                                                   >  2:70988/1‑216 (MQ=255)                                       cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGt                                                                                                                                                                                                                   <  1:70988/216‑1 (MQ=255)                                         aTCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCACGCCACCGCGGCGCAATAGCGTGTCGATCTTCAATGCAATGGATCGCGTCGGTACCCGCTCTGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGGAATATGCTGGATACGCAAAATCCCATGTACCAGCAAATTGCCGCTAACGTGGTCACGTTCTTATTGCAAGAAATGGCCCATGGGCGt                                                                                                                                                                              >  2:292385/1‑251 (MQ=255)                                                  ttGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTCTGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGCTAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGCAATGGCGCATGGGCGTATTccgccg                                                                                                                                                                     >  1:343679/1‑251 (MQ=255)                                                             gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCATGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATAGATCCGAAAAAGATTGTCGCCGTAGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGcc                                                                                                                                                          >  1:165355/1‑251 (MQ=255)                                                                          ttCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGc                                                                                                                                             >  1:320872/1‑251 (MQ=255)                                                                             cTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCCATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTg                                                                                                                                          >  1:44730/1‑251 (MQ=255)                                                                                 cgcgcAACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGc                                                                                                                                       >  1:71515/1‑250 (MQ=255)                                                                                              ggcAATAGCGAGGCGATCTTCCATGCAAGGTATCGCGTCGGTCCCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTTTAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGTATAAGCAAAAGTCCAGGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTAGTGCAGGAAATGGCGCATGGTCGTATTCCGGCGGAATTGCTGCCGCTGCTAAGTGGCGGGGGCAATATCTGTAATGc                                                                                                                         <  2:36441/250‑1 (MQ=255)                                                                                                 aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGt                                                                                                                      <  1:11772/251‑1 (MQ=255)                                                                                                        tgtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGAAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATTCGGTAATGgcg                                                                                                               >  1:241497/1‑251 (MQ=255)                                                                                                         gtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGgcgc                                                                                                              >  1:207064/1‑251 (MQ=255)                                                                                                                  ccATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTggggg                                                                                                     <  2:320872/251‑1 (MQ=255)                                                                                                                  ccATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTggggg                                                                                                     >  2:241547/1‑251 (MQ=255)                                                                                                                                  gTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTcc                                                                                                                                                                         >  1:102622/1‑167 (MQ=255)                                                                                                                                  gTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTcc                                                                                                                                                                         <  2:102622/167‑1 (MQ=255)                                                                                                                                  gTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGAGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATAACAAAAATGCGGTAATGGCGCGTCTGGGGGAGAACCCGGTAATtcc                                                                                     >  1:201713/1‑251 (MQ=255)                                                                                                                                   tCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGCACACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGGAAACCCGGTAATtcct                                                                                    >  1:166395/1‑251 (MQ=255)                                                                                                                                                tgtgCAAATCGATCCGAACAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGATAATTCCTCCGTTTATGATGt                                                                       >  2:43761/1‑251 (MQ=255)                                                                                                                                                 gtgCAAATCGATCCGCAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGt                                                                       >  2:119997/1‑250 (MQ=255)                                                                                                                                                         cGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCggc                                                                >  2:61040/1‑248 (MQ=255)                                                                                                                                                          gATCCGAAAAAGATTGTCGCCGTCGTGGCAACCCACTTGCGCGCAC‑AAGGTGATATGCTGGATACGCAAAATCACATGTGCCAGCAAATTGACGATAACGTGGTCACGTTCTTATTGCAGGGAATGGCGCATGGGCGTATTCAGCCGGAATTTCTGCCGCTGCAAAGCGGCGTGGGCAATCTCAATAAGACGATAATGGCACGTCTGGGGGTAAAAACGGGAATTCCTCCGGTTAAGATGTATTCggcggg                                                             >  2:199348/1‑247 (MQ=255)                                                                                                                                                                      aTTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCTCGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc                                                 <  2:241497/251‑1 (MQ=255)                                                                                                                                                                       ttGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGGGTGGGGGAAAACCCGGTAAATCCTCCGTTTATGATGTATTCGGAAGTGCTACCGGAATCg                                                >  2:168981/1‑251 (MQ=255)                                                                                                                                                                       ttGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACACCGGTAATATGCTGGATAAGCAACATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGCGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAACCGGGAATTCCTCCGTTTATGGTGTATTGTGAAGGGCTAAAGGAATCg                                                >  2:168986/1‑251 (MQ=255)                                                                                                                                                                             ccGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGgtggtg                                          <  1:196113/251‑1 (MQ=255)                                                                                                                                                                                      aaaCCGACTTGCCTG‑ACGCAGGTAATATGCTGGCCAACCCAAATCCCATGTGCCACCCCATTGCCGATAACGTGGTCACCTTCTTATTCCAGGAAATGGCGCATGACCTTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCACTATCAATAAAGCCACAATCGCGCGGCTCCGGGAAACCCCGGGAATTCCTCCGCTTATGCTGTATTCGGAGGTGCtcctggaaccgggggggaatttacac                                 >  2:323388/1‑226 (MQ=255)                                                                                                                                                                                       aaCCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCAGTGGTGCATTTACTgg                                <  1:61040/251‑1 (MQ=255)                                                                                                                                                                                                   cG‑ACGGCGGTAATATGGTGAAGAAGCAAAATCCCAAGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAAGTGCTGCCGCTGCAAAGTGTCGTGGGCAATATCAATAATGCGGGAATGGCGCGTCTGGGGTAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGGAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaaaa                    <  1:115897/251‑1 (MQ=255)                                                                                                                                                                                                         ccGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGc               >  1:133024/1‑251 (MQ=255)                                                                                                                                                                                                          cGGTAATATGCTGGATAAGCAAAATCCCATGTGACAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTACGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACACGGTAATTTCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGGGCATTTACTGGAAAACGGCAAAATCAGCg              >  1:286309/1‑251 (MQ=255)                                                                                                                                                                                                           ggTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCgg             <  1:213901/251‑1 (MQ=255)                                                                                                                                                                                                             tAATATGCTGGATCAGCAAAATCCCCTGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTg                                                                                                                                          >  2:332742/1‑124 (MQ=255)                                                                                                                                                                                                             tAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTg                                                                                                                                          <  1:332742/124‑1 (MQ=255)                                                                                                                                                                                                                      ggATAAGCGAAATCCCATGTGCCAGCAAATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGACGTATTCCGCCGGAATTTCTGCCGCTGCACAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGATTATGATGTATTCGGAAGGGCTACAGGAATCGGGGGTGCATGTTCTGGGAACCGGCAAAATCAGCGGGGCCAGCGccc  >  2:85272/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                CGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG‑ACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCT  >  NC_000913/3065258‑3065720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑