Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,039,623	G→A	H41Y (CAT→TAT)	xerD ←	site‑specific recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,039,623	0	G	A	100.0%	135.3 / NA	40	H41Y (CAT→TAT)	xerD	site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/23);  total (17/23)

GTAATAAACGTTCGACCTGCGCTTCGCTTAAATCTTTTGGCAAACGCTGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATGCAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGT  >  NC_000913/3039377‑3039866                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gTAATAAACGTTCGCCCTGCGCTTCGCTTAAATCTTTTGGCAAACGCTGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAAc                                                                                                                                                                                                                                                 >  1:60126/1‑251 (MQ=255)                         cgcttAAATCTTTTGGCAAACGCTGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATc                                                                                                                                                                                                                          <  1:280172/250‑1 (MQ=255)                            ttAAATCTTTTGGCAAACGCTGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAACCTTTTCGCGATAAAGATACTGGAACAATCGGCGCCCTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGAGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATcgcg                                                                                                                                                                                                                       >  1:341312/1‑250 (MQ=255)                                      tGGCAAACGCTGGGGCCATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGAAAGATCGCGACGGTAAGCGt                                                                                                                                                                                                            >  1:17687/1‑251 (MQ=255)                                            aCGCTGTGGCAATGTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAATCTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACGCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCCCCATCATTGACAGATCGCGACGGTAAGCGTGCAAct                                                                                                                                                                                                      <  2:208651/251‑2 (MQ=255)                                                tGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTAtt                                                                                                                                                                                                  >  1:62237/1‑251 (MQ=255)                                                 ggggCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTAttt                                                                                                                                                                                                 <  1:341730/251‑1 (MQ=255)                                                       aTTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCGCCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGcc                                                                                                                                                                                           <  1:173648/251‑1 (MQ=255)                                                                   cGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCCGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTcc                                                                                                                                                                               >  1:195861/1‑251 (MQ=255)                                                                          cgcACTGAGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGCATTTTCAGCCAGATTTTTTTCCAGCcac                                                                                                                                                                         >  1:73868/1‑250 (MQ=255)                                                                             ccTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATCCCCGCCCTCCAGCCGTTCCGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACagat                                                                                                                                                                     <  2:195861/250‑2 (MQ=255)                                                                             aCTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACag                                                                                                                                                                       >  2:277775/1‑249 (MQ=255)                                                                                     cGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCa                                                                                                                                                                >  1:126191/1‑248 (MQ=255)                                                                                                      tCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCTATGCGTg                                                                                                                                             <  1:87726/250‑1 (MQ=255)                                                                                                        gcgATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCa                                                                                                                                          <  1:134288/251‑1 (MQ=255)                                                                                                         cgATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGcc                                                                                                                                           >  2:86261/1‑249 (MQ=255)                                                                                                                 aTACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGCAACTGCTCGATGCGTGCCAGATCCTGtt                                                                                                                                 >  2:236956/1‑251 (MQ=255)                                                                                                                                       gcactCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTGCTGCCAGTAATGCCTTCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCa                                                                                                           <  1:255453/251‑1 (MQ=255)                                                                                                                                             aGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACGTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCGCCAGCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGGGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTGGCGGCCCTTATGTGCACTCAtt                                                                                                     <  2:341312/251‑1 (MQ=255)                                                                                                                                                aaaCGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTg                                                                                                   >  1:145642/1‑250 (MQ=255)                                                                                                                                                    gcgcTGAGCTGGTGGCTTTATCCCCGCCCTCCAGCCTTTCTGCCAGTAATGCCTGCAAATCTTCACGTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCTTTCAACGTATTTGCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGAGGCGTGCCATATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCa                                                                                              <  1:224046/251‑1 (MQ=255)                                                                                                                                                      gcTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCAtt                                                                                            <  2:145642/251‑1 (MQ=255)                                                                                                                                                          aGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCATTAATGCCGGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCTCCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACATAGCATCAGGAAACTGCGCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGc                                                                                        <  2:124060/251‑1 (MQ=255)                                                                                                                                                          aGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTACAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGc                                                                                        <  1:192391/251‑1 (MQ=255)                                                                                                                                                                     ttATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGc                                                                              <  1:52810/250‑1 (MQ=255)                                                                                                                                                                      tataCCCTCCCCCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGGAAGCGTTCAACTTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCGCTTGCGCCTCTTAGGGTCACTCATTGGATCCATTATGCCTTATTGTGcc                                                                             <  2:175962/250‑1 (MQ=255)                                                                                                                                                                         aCCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGTGCCCCTTATGGTCTCTCATTTGATCCATTATGCCTTATTGTGCCGTg                                                                          <  1:310440/250‑1 (MQ=255)                                                                                                                                                                          cccGCCCTCCCGCCGTTCTGCCAGTAATGCCTGCAAATCGTCCCTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACCACAACTCCACCATCATTGACAGATCGCGCCGGTCAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCCAGAAACTGCGCGATGCGTGCCCAATCCTGTTTCACTTGCGCCCCATGTGGAAACTCATTTGATCCTTTATGCCTACTTGTTCCGTGAc                                                                        >  2:280535/1‑251 (MQ=255)                                                                                                                                                                                cTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTgc                                                                                                                         <  1:213532/196‑1 (MQ=255)                                                                                                                                                                                cTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTgc                                                                                                                         >  2:213532/1‑196 (MQ=255)                                                                                                                                                                                    aGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCCACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGAtt                                                              >  1:140716/1‑251 (MQ=255)                                                                                                                                                                                                   aaTGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTGCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGcc                                                <  1:352353/250‑1 (MQ=255)                                                                                                                                                                                                    aTGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCTTTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGCCGc                                              >  2:71227/1‑251 (MQ=255)                                                                                                                                                                                                            aaaTCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCAACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGc                                                                              >  1:200848/1‑211 (MQ=255)                                                                                                                                                                                                            aaaTCGGCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGc                                                                              <  2:200848/211‑1 (MQ=255)                                                                                                                                                                                                                        tGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCaa                                                                                                                                                               >  2:131783/1‑118 (MQ=255)                                                                                                                                                                                                                        tGCGCCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCaa                                                                                                                                                               <  1:131783/118‑1 (MQ=255)                                                                                                                                                                                                                           gcCGTCGCCAGCGTCAACCCGCGGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGaa                       <  2:186746/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAGCGTCAACCCGCGGTGATACACCCCCTCCACCATCATTGACAGTGCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCCCTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGTTACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGt               <  2:238733/251‑1 (MQ=255)                                                                                                                                                                                                                                                cgGTGATACAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGt  <  1:267260/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GTAATAAACGTTCGACCTGCGCTTCGCTTAAATCTTTTGGCAAACGCTGGGGCAATTTCGGTGAAGCGAGATGCGCACTGGGATCGTCTTCACGAAACTTTTCGCGATAAAGATACTGGAACAATCGGCGCACTGCACTCAGCAAACGCGCTGAGCTGGTGGCTTTATACCCGCCCTCCAGCCGTTCTGCCAGTAATGCCTGCAAATCGTCACTTTGCGCCGTCGCCAGCGTCAACCCGCGGTGATGCAACCACTCCACCATCATTGACAGATCGCGACGGTAAGCGTTCAACGTATTTTCAGCCAGATTTTTTTCCAGCCACAGAGCATCAAGAAACTGCTCGATGCGTGCCAGATCCTGTTTCACTTGCGCCCCTTATGGTCACTCATTTGATCCATTATGCCTTATTGTGCCGTGACTAAAGCGATTCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGT  >  NC_000913/3039377‑3039866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑