Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,832	C→T	R6C (CGC→TGC)	yqfG →	uncharacterized protein YqfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,832	0	C	T	100.0%	49.2 / NA	15	R6C (CGC→TGC)	yqfG	uncharacterized protein YqfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/5);  total (10/5)

ACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGCGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGATAAACGCTCACTTGGTTAATCATTTCACTCTTCAATTATCTATAATGATGAGTGATCAGAATTACATGTGAGAAATTATG  >  NC_000913/3032585‑3033067                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             aCGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATgtgcg                                                                                                                                                                                                                                           <  1:351702/251‑1 (MQ=255)                  cttttctAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCtgttg                                                                                                                                                                                                                          >  1:58650/1‑251 (MQ=255)                     ttctAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTgttgtt                                                                                                                                                                                                                        <  2:103246/250‑1 (MQ=255)                                 ggCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTActctc                                                                                                                                                                                                           >  1:143365/1‑251 (MQ=255)                                                gctgctACATAGCGGCATTATGCTCGCAACAATTACCGCCGTAGTCATGCACGACTTCTTTAATGGCCAACAGCATCATGCCGCCCTGGTGAAGGAATACGTATCTGATCAAGATTTAAAAATAAGACCCGTACGTATGTGGCTTATGATGCGCAAGCTGAAGAAAAATCAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCAtt                                                                                                                                                                                            >  1:326186/1‑251 (MQ=255)                                                          aGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACt‑gtg                                                                                                                                                                                 >  1:270170/1‑251 (MQ=255)                                                                                                gAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATatgatg                                                                                                                                           >  1:286286/1‑251 (MQ=255)                                                                                                gAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATGTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATGTCTGACT‑GTGTTGCAAGGGCCATTGAAAGTCGCTTCAAATATatgatg                                                                                                                                           <  1:17974/251‑1 (MQ=255)                                                                                                            tAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGATTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACCCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTa                                                                                                                                >  1:341579/1‑250 (MQ=255)                                                                                                             aaTGGCTATCAGCATCATGCTGACCTGGTGAAGAAATCCGTACCTGATCAAGACCTAAAAGTCAGGACAGTACGTATGTGACCTCTGATGCGAAAGAAGAAGAAAAATGCACATGGAGAATACTATGAAATTTTTAATGTGCGCTATATTCAGTCTGTTGCTGCTTTTTCATCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTaa                                                                                                                               >  1:166866/1‑250 (MQ=255)                                                                                                                                                                               aCAGTACGTATGTGGCTTCTGATGCGCAAGCTGACGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGATAAACGCTCACTTGGTTAATc                                                             >  2:320534/1‑250 (MQ=255)                                                                                                                                                                                                                aataaaaaTGAGCATGGTGAATAATATGAATTTTTTATTGTGCGCTATATGCAGTCTGTTTTTTCTTTTTTCTCTCTCTATTCCTTTCATTTCTGACTCGT‑TTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAAGGTTTTTTCCCTGTCGGCATCCGCATAATACGGGCGGTTGTCGATAAACGCTCCCTTGGTTAATCATTTCACTTTTCAATTATCTATAATGAAGAGTg                            <  2:11676/247‑1 (MQ=255)                                                                                                                                                                                                                             aTGGAGAATAATATGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGATAAACGCTCACTTGGTTAATCATTTCACTCTTCAATTATCTATAATGATGAGTGATCAGAATTACAtg              <  2:270170/251‑1 (MQ=255)                                                                                                                                                                                                                                gagaATACTATGAAACTTTTAATGTGCGCCCCAATCACTCTGTTGTCACATTTAACTCTCTCTATTCCTATCATTTCTGACT‑GTGTTGCAAAGGACATTGAAAGTAGCTTCAACTATCTGATGCTACTTTTTTCATTGGTTTTTAACTGCACGCATCCGCTCAAAACGGGCGGTTGTCGATAAACACTCACTTGGATAATCATTTCACTCTTCACTTATCTATCATGCTGAGTGATCAGAATTACAtgtg            >  2:328837/1‑250 (MQ=255)                                                                                                                                                                                                                                      aataTGAATTTTTTAATGTGCGCTATATTCCGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGACAGTCGCTTCAAATATATGATGCTCCTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGGTAAACGCTCACTTGGTTAATCATTTCACTCTTCAATTATCTATAATGATGAGTGATCAGAATTACATGTGAGAAAtt     >  2:127889/1‑251 (MQ=255)                                                                                                                                                                                                                                         atGAATTTTTTAATGTGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGATAAACGCTCACTTGGTTAATCATTTCACTCTTCAATTATCTATAATGATGAGTGATCAGAATTACATGTGAGAAATTATg  <  1:320534/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             ACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAAAATGAGCATGGAGAATAATATGAATTTTTTAATGCGCGCTATATTCAGTCTGTTGTTGCTTTTTACTCTCTCTATTCCTGTCATTTCTGACT‑GTGTTGCAATGGCCATTGAAAGTCGCTTCAAATATATGATGCTACTTTTTTAAATGGTTTTTACCTGTCGGCATCCGCTCAAAACGGGCGGTTGTCGATAAACGCTCACTTGGTTAATCATTTCACTCTTCAATTATCTATAATGATGAGTGATCAGAATTACATGTGAGAAATTATG  >  NC_000913/3032585‑3033067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑