Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,937,789	G→A	A108T (GCA→ACA)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,937,789	0	G	A	94.9%	110.3 / ‑1.1	40	A108T (GCA→ACA)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (13/24);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

TGTTGCCCGCGCCTCAACGCCTAATGCCAGCGATATCGCGATGGAAAACAACACCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAACCAGCCAGATGCTGGATATCCAGC  >  NC_000913/2937548‑2938032                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    tgtggCCCGCGCCTCAACGCCTAATGCCAGCGATATCGCGATGGAAAACAACACCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAAGGCCACATCCGCTGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAATCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGtt                                                                                                                                                                                                                                             <  2:304535/246‑1 (MQ=255)                 aCGCCTAATGCCAGCGATATCGCGATGGAAAACAACACCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACg                                                                                                                                                                                                                            >  2:294672/1‑251 (MQ=255)                          gCCAGCGATATCGCGATGGAAAAAAAAACATAGCACCAGCGGTCTTTAGACGCCACTTTGCAACGATTAGCCGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCTGTATCGCCGTCACCAACTTTGGTGCGGATGGCGCTCTGGTAGATTAGCCAGGCAATCTGCTCTCTCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGCCAATATTGAATGGTTAAtatc                                                                                                                                                                                                                   >  2:358286/1‑248 (MQ=255)                              gCGATATCGCGATGGAAAACAACACCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGca                                                                                                                                                                                                               <  1:102771/251‑1 (MQ=255)                                      gcgATGGAAAACAACACCGGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTGGCTGAGTGCTGTCTGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAAGCTGCTGTATCCGATTAGTAGCTGTAAATGTCCGCGAACAACAGCGGTGATGGACAATATTGAACTGTTAATCTCCGCGCATCGGtt                                                                                                                                                                                                       <  2:340920/251‑1 (MQ=255)                                                 caacaCCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCTAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAAGCTGCTCTATCCGATTAGTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGTCTAttt                                                                                                                                                                                            <  1:287751/251‑1 (MQ=255)                                                   acacCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTAGCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATGGAACGGGTAATCTCCGCACAGCGGTTGCAGGCTATTTCt                                                                                                                                                                                          <  1:301855/251‑1 (MQ=255)                                                         ggCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCTAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCg                                                                                                                                                                                      <  1:40422/249‑1 (MQ=255)                                                                          gACGCCATTTTGCAACGCTTTGATGATTGCTGCCGGCAAATCCATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATCAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTcc                                                                                                                                                                    >  1:110436/1‑249 (MQ=255)                                                                             gCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATAc                                                                                                                                                                >  1:72662/1‑251 (MQ=255)                                                                              ccATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                                                                                                               <  1:237331/251‑1 (MQ=255)                                                                              ccATTTTGCAAAGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                                                                                                               >  2:139564/1‑251 (MQ=255)                                                                                aTTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGCCTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGCTGGCGCTCTGGTAGACAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGtt                                                                                                                                                             >  1:157458/1‑251 (MQ=255)                                                                                  tttGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTta                                                                                                                                                            <  1:34751/250‑1 (MQ=255)                                                                                       aaCGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                                                                                                                        >  1:81306/1‑249 (MQ=255)                                                                                               gCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtgt                                                                                                                                               <  1:222048/250‑1 (MQ=255)                                                                                               gCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGtgtg                                                                                                                                              <  2:281528/251‑1 (MQ=255)                                                                                                        tGTCGGCAAATCAATAGGGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaag                                                                                                                                      <  2:110436/250‑1 (MQ=255)                                                                                                             gCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatc                                                                                                                                >  2:195627/1‑251 (MQ=255)                                                                                                                          gAACTGATGGAATGCCACATCCGCGGTATCGCCGGCACCACCTTTGTTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGTTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGTGTCGGATCCTTTAGTTGCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgct                                                                                                                   <  2:169576/251‑1 (MQ=255)                                                                                                                              tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGa                                                                                                                >  1:91833/1‑250 (MQ=255)                                                                                                                                   gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgc                                                                                                          <  1:195627/251‑1 (MQ=255)                                                                                                                                                       cGCCGTCACTCCCTTTGGTGTTGCTGGCTCTCTTGTAGATAAGCTAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGTACAACAGCTGATATGGAAAATATTTAACGGTTAATCTCCGCACAGCTGTTGCAGGCTATTTCTGGCGTCGGATCCTTTAGTTTCAATACGTTATATAAGTTGTTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACCCGCGCACGCCTTGCTCTTTAttt                                                                                      <  2:62511/251‑1 (MQ=255)                                                                                                                                                        gccgtatccaccTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCGGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATTGACAATATTGAACGGTTAAGCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGGTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTtc                                                                                     <  2:56961/244‑1 (MQ=255)                                                                                                                                                           gTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTtcgtc                                                                                  >  1:162473/1‑251 (MQ=255)                                                                                                                                                                       ggTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAacc                                                                       >  1:284209/1‑250 (MQ=255)                                                                                                                                                                        gtgtGGAAGGCGCTATGGTGGAAAAGCAAGGCAATCTGTTCTATCCAATTATTAGCTTGAAATGTCCGCGAACAACAGCGTTTATGGCCAATATTGAACGGTTAATCTCCGCATAGCGTTTGCAGGCTATTTCTGGCGTCGGAGCCGTTATTTTCAATACGTTATATAAGTTGGTTTGGTTGAAAGTAAAGCATCCACAACTGCTGGAACGCGCGCACGCCTGTCTCTTTATTTCGTCGATGATTAaccac                                                                     <  2:203638/251‑1 (MQ=255)                                                                                                                                                                                           aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCa                                                  <  2:107096/251‑1 (MQ=255)                                                                                                                                                                                                      gCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTTGTGTGGTTGAAAGAAAATCATCCACAACGGCTGGAACGCGCGCACGCCTGTCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATc                                       <  1:223695/251‑1 (MQ=255)                                                                                                                                                                                                             gCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATg                                 <  2:219893/250‑1 (MQ=255)                                                                                                                                                                                                                  aTCCGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCg                             <  1:294672/249‑1 (MQ=255)                                                                                                                                                                                                                     cGATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATGTTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAAcc                        >  1:140067/1‑251 (MQ=255)                                                                                                                                                                                                                      gATTATTAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGT‑ATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCAACGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAAcc                        >  1:26031/1‑249 (MQ=255)                                                                                                                                                                                                                       attattAGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACTGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGTAACTCGCGCTCGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGGGAATTCACTACTGATATCACGATGGCCGGAAccag                      <  1:42159/251‑1 (MQ=255)                                                                                                                                                                                                                       attattAGCTGGAAATGTCCGCGAACAACAGCGGTTAGGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGGCTTTAGTTTCAATACGTTATAGAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAGCCACCTTTTATCCGGCGAAGTCACTACTGATATCACGATGGCCGGAAccag                      <  1:16264/251‑1 (MQ=255)                                                                                                                                                                                                                          attaGCTGGAAATGTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAAccagcca                   <  1:36447/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggccGCGAACAATAGTGGTTATGGATAATAATTAACGGGTAATCTCCGATTAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCTACATCTGCTGGAACGAGCTCTCGCCTGGCTCATTATTTCGTCGCTTATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGTCGGAACCAGCCAGATGCTGGagat       <  1:285806/248‑4 (MQ=255)                                                                                                                                                                                                                                       gTCCGCGAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTGCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAACCAGCCAGATGCTGGatat       <  1:209032/250‑1 (MQ=255)                                                                                                                                                                                                                                          cgcgAACAACAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAACCAGCCAGATGCTGGATATcc     >  1:303077/1‑249 (MQ=255)                                                                                                                                                                                                                                           gcgAACACCCGCGGTTATGGACAATATTGAACGGATAATCTCCGCACAGCGGTTGAAGGCTCTATATGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAACCAGCCAGATGCTGGATATCCAGc  >  2:144616/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    TGTTGCCCGCGCCTCAACGCCTAATGCCAGCGATATCGCGATGGAAAACAACACCTGGCACCAGTGGTCTTTAGACGCCATTTTGCAACGCTTTGCTGATTGCTGTCGGCAAATCAATAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGGCTCTTTATTTCGTCGCTGATTAACCACCGTTTAACCGGCGAATTCACTACTGATATCACGATGGCCGGAACCAGCCAGATGCTGGATATCCAGC  >  NC_000913/2937548‑2938032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑