Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,930,364	G→A	W263* (TGG→TGA)	sdaB →	L‑serine deaminase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,930,364	0	G	A	100.0%	194.9 / NA	58	W263* (TGG→TGA)	sdaB	L‑serine deaminase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/28);  total (30/28)

AGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGGATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTG  >  NC_000913/2930120‑2930605                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAAc                                                                                                                                                                                                                                               <  1:90381/251‑1 (MQ=255)        cGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTg                                                                                                                                                                                                                                        >  2:110813/1‑251 (MQ=255)            cTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATAAACATGTTTGCACt                                                                                                                                                                                                                                    >  1:89149/1‑251 (MQ=255)                       tGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGACCGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACg                                                                                                                                                                                                                         >  1:345274/1‑251 (MQ=255)                        ggCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACg                                                                                                                                                                                                                         <  1:261599/250‑1 (MQ=255)                        ggCCCGATGATGAAAAACGAGCGTGCGCTGCACAGCAACGGCTAGCCGTAACAGATCCTGGCGAACGTCTGGGAAGTCTTGCGCTGCGGTATTGAGCGCTGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCGGCGGAACTACGCCTGATGCTGGTCAGTCAGGATAAAACCACCGCTGACCCGATGTCGGTTGTTGACGGAAGTAACATGTTTGCACTTGCAGTGAACGa                                                                                                                                                                                                                        <  2:33878/251‑1 (MQ=255)                                       aaCGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGCACGACTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTgg                                                                                                                                                                                                         >  1:309504/1‑251 (MQ=255)                                          gAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGATGCTGGCgg                                                                                                                                                                                                      >  1:274945/1‑251 (MQ=255)                                            gCTGGCGCTGCACAGCAAAGAAGCGCTGGAACAGCACCTGGCGATCGTCTGGGACGTCATGCGCGGCGGTATTGATCGCGGTATTTCAACCGAAGGCGTGTTGCCTTGCAAACTGCGCGTTCCACGCCGTGCTGCGGCCCCACGCCGGATGCTGGTCAGCCAGGATAAAAACACCACTGACCCGATGGCGGTTGTTGACTGAATCGACACTTTTGCACTGGCCGTGAACGAAGAGaaagagggtggcgtgg                                                                                                                                                                                                    >  2:70615/1‑237 (MQ=255)                                                                     cTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGGGACTGCGCCGACTAACgg                                                                                                                                                                           >  1:60581/1‑251 (MQ=255)                                                                            aGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGgtgcgtgc                                                                                                                                                                    <  1:154752/251‑1 (MQ=255)                                                                                      gAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATcc                                                                                                                                                          <  2:280728/251‑1 (MQ=255)                                                                                      gAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGGGCGTGCGGGATTATcc                                                                                                                                                          >  1:290676/1‑251 (MQ=255)                                                                                       aaCGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCGCCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAAGCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATccc                                                                                                                                                         <  2:331331/251‑1 (MQ=255)                                                                                             tGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGCGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGAGTGCGGGATTATCCCGGCAG‑t                                                                                                                                                  >  1:323197/1‑251 (MQ=255)                                                                                                gAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCt                                                                                                                                               <  2:181752/251‑1 (MQ=255)                                                                                                gAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAAAGGTGCGTGCGGGGTTATCCCGGCAG‑TTCt                                                                                                                                               >  2:88253/1‑251 (MQ=255)                                                                                                         cgcgGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACGGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCGGGCGtacta                                                                                                                                      <  2:345274/251‑1 (MQ=255)                                                                                                           cggcggTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACg                                                                                                                                    <  2:273216/251‑1 (MQ=255)                                                                                                             gcggTATTGAGCGCTGAATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGCCTGAATCAACATTTTTGCACTGGCAGTGAACGAAGAGACCGCTGCTGGCGGTCGCGTGGGGTCTGGGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACgcc                                                                                                                                  >  2:195197/1‑249 (MQ=255)                                                                                                                gTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAg                                                                                                                               <  2:25717/251‑1 (MQ=255)                                                                                                                 tATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGAATGCGCCGACTAAAGGGGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGt                                                                                                                              >  1:45711/1‑251 (MQ=255)                                                                                                                     gAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGGGCGGGATTATCCCGGCCG‑TTCTGGCGTTCTACGACAAGTTTAt                                                                                                                          >  2:89290/1‑251 (MQ=255)                                                                                                                            gTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGaa                                                                                                                   <  1:83380/251‑1 (MQ=255)                                                                                                                              aTTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGGGCGTGCGGGGTTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAgg                                                                                                                 >  1:177511/1‑250 (MQ=255)                                                                                                                              aTTTCCACCGAAGGCGTGTTGCCTGGCAAACGGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGAGTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGt                                                                                                                 <  2:178351/251‑1 (MQ=255)                                                                                                                                 tCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGGGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑GTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGa                                                                                                               >  1:57739/1‑250 (MQ=255)                                                                                                                                   cACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAct                                                                                                            <  1:260234/251‑2 (MQ=255)                                                                                                                                   cACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑g                                                                                                           <  1:270162/251‑1 (MQ=255)                                                                                                                                   cACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑g                                                                                                           <  1:260236/251‑1 (MQ=255)                                                                                                                                    aCCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑GTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑Gc                                                                                                          >  1:176094/1‑251 (MQ=255)                                                                                                                                               gtTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATGAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGGGGGGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGAGAAA‑GCTAACTCACTgg                                                                                               >  1:14639/1‑251 (MQ=255)                                                                                                                                                ttGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGc                                                                                              >  1:72037/1‑251 (MQ=255)                                                                                                                                                  gCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCt                                                                                             <  1:110813/250‑1 (MQ=255)                                                                                                                                                      ggCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGtt                                                                                         >  1:132281/1‑250 (MQ=255)                                                                                                                                                               cgcgTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGCCTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGAGAAC‑GCTAACTCACTGGCTCGTTACCTGCTggg                                                                               >  1:356068/1‑250 (MQ=255)                                                                                                                                                               cgcgTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGt                                                                               <  1:89290/251‑1 (MQ=255)                                                                                                                                                               cgcgTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGGCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGt                                                                               <  1:195251/251‑1 (MQ=255)                                                                                                                                                                         gCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAATAGAACGCTGCTGGCGGTCGCGGGGGGACTGGGACGACTAACGGGGCCTGCGGGCTTATCCCGGCAGGTTC‑GTCGTACTACGAAAAGGTTATCCGCGTAGTGAAC‑GCTAACTCACTGGCTCGTTAACTGCTGGTAGCCAGAGcc                                                                     >  2:68099/1‑251 (MQ=255)                                                                                                                                                                          ccGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGCTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGGGACTGCGCCGACTAACGGTGCGTGCGGGACTATCCCGGCAC‑ATCTGGCGTACTACGACAAAGTTATCCCCGAAGAGAACCGC‑AACTCCCTGGCTccatccccgccggtaaccaatgccc                                                                    >  2:121439/1‑227 (MQ=255)                                                                                                                                                                            gTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGGGGTGACTGCGCCGACTAACGGAGCGGGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCCCTGGCTCGTTAACCGCTGGTAGCCCGCGccctt                                                                  >  2:105447/1‑248 (MQ=255)                                                                                                                                                                              gaggcGGCCCTACGCTGTATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAACCAACATGTTTGCACGTGTAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGGGGGATTATCCCGGCAT‑TTGTGGCGGACTGCGCCAAGTTTATTCGCGTAGTGAAC‑GATAACTCGCGGGCTCGTTACCTGATGGTAGCCAGCTCCATTgg                                                                <  2:44481/248‑1 (MQ=255)                                                                                                                                                                                 gcGGCACGACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGt                                                               <  2:171599/249‑1 (MQ=255)                                                                                                                                                                                          cGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATaa                                                    <  1:66089/251‑1 (MQ=255)                                                                                                                                                                                            ccGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGa                                                  <  2:352095/251‑1 (MQ=255)                                                                                                                                                                                              ggATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATTTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTTCTACGACAAGTGTAGCCGCGAAGTGAAC‑GCTAACTGACTGGCTCGTTACCTGGTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATg                                                <  2:201360/251‑1 (MQ=255)                                                                                                                                                                                              ggATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGGTTATCCCGGCAG‑TTCTGGCGTACTACGACAAGGTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTTCCTTCTGGTAGCCCGCGCCATTGGTTCTCTTTTTAAGATg                                                >  2:121522/1‑251 (MQ=255)                                                                                                                                                                                                gtGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑TCTAACTCACTGGCTCGTTACCTGCTGGGAGCCAGCGCCATTGGTTCTCTTTATAAGATGaa                                              <  2:177451/250‑1 (MQ=255)                                                                                                                                                                                                aTGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGGTTATCCCGGCAG‑GTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTTTAAGATGa                                               >  1:293335/1‑250 (MQ=255)                                                                                                                                                                                                 tGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGCCTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGGCAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAAc                                             >  1:307777/1‑251 (MQ=255)                                                                                                                                                                                                    tGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGGGGTGACTGCGCCGACTAAAGGAGCGTGCGGGCTTATCCCGGCAG‑TTCTGGCGTACTACGACAAGGTTATCCGCGAAGTGAAC‑GCTAACTCCCTGGCGCGTTACCTGCTGGTAGCCAGCGCCCTTAGTTCTCTTtaataaatgaaagcg                                          >  2:321917/1‑238 (MQ=255)                                                                                                                                                                                                                  aaaaCCACCACTGACCCGATGGCGGTTGTTGACTGAATCAACAGGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCTACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGGGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCGTCGATTTCTGGTGc                            <  2:200169/251‑1 (MQ=255)                                                                                                                                                                                                                             tGACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGACTAGAACGCTGCTGGCGGTCGCGGGGTGGCTGCGCCGACTAACGGTGCGTGCGGGATTATTCCGGACG‑TTCTGGCGTACCACGACAAGTTTATCCGCGAAGGGAAC‑GCTAACTCACTGGCTCGGTACCTCCCGGTAGCCCGCGCCCTTGGTTCTCTTTTTAAACTGAACACGTCggatttttgggccgaagcgggtt                 >  2:95274/1‑229 (MQ=255)                                                                                                                                                                                                                              ggcccGATGGAGGTTGTTGACTGAATCAACATTGTTGCACTGGCAGTGAACGAAGAGTACGCGGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCTTACTACGACAAGTTTATCCGCGAAGTTAAC‑GCTAACTCACTGGCTCGTTACCTTCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCTTCGATTTCTGGTGCGGAAgttggttg                <  2:312095/249‑1 (MQ=255)                                                                                                                                                                                                                              gACCCGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCGGCGATTTCTGGTGCTGAAGTGGGTTg                >  1:312699/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccGATGGCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTGCCTGCTGGTAGCCAGCGCCATTGGTGCTCTTTATAAGATGAGCGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCa             <  2:177511/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggCGGTTGTTGACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTg        <  2:57739/251‑1 (MQ=255)                                                                                                                                                                                                                                            tgttgACTGAATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGATGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGAGCGTGCGGGGTTATCCCGGCAG‑TTCTGGCGTAATACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAAATGAACGCGTCGATTTCTGGTGCTGAAGGGGGTTGCCCGGGTGAAGTtg  >  1:215335/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCTGCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGGATCAACATGTTTGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGTCGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCCCGGCAG‑TTCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAAC‑GCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGCGCCATTGGTTCTCTTTATAAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTG  >  NC_000913/2930120‑2930605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑