| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,913,761 | G→A | L414L (CTG→TTG) | rlmD ← | 23S rRNA m(5)U1939 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,913,761 | 0 | G | A | 92.9% | 80.1 / ‑5.3 | 28 | L414L (CTG→TTG) | rlmD | 23S rRNA m(5)U1939 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (10/16); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACA > NC_000913/2913519‑2913922 | gTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCTCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGcc < 1:165823/250‑1 (MQ=255) gACAATACGCCCGGGTTCCGGCTAAGCACCCCCACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTcgcg < 2:252131/251‑1 (MQ=255) gACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTcgcg > 1:6553/1‑251 (MQ=255) tCGGCTAAGCACTCACACGACTTCTGGTTGGGAATACCCAGACTTGGGATCCATTTTCCCGGATCAAATTCACCAGCCTTAGTGATATGGG‑CTCTTCTTACCGCAACCATCGTCCTCTCCTGTAGGAACCAGCCCAGCCGAAATCGGCAAATCGCATCTATTTAACGCTCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTTTGTGAACATAGCTAACATCGCCAGTCGCGCAATGGTATATCCTGc < 1:296152/250‑1 (MQ=255) cacGACTTCTGGATGGTAATACCCAGACTTTCTATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAGCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACAGCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCg < 1:39930/251‑1 (MQ=255) cacGACTTCGGGTTGGTAATACCAAGACTTGCGATCAATTTTTCCGGAACAATTTCACCAGCCTTATTGATAGGTG‑CACTTCTTCCCCCAACCATCGTCCCCCCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATGCCAGATGTCCCGTGTGTGGGAACATATCCAACAGCGCCAGTCGCGCAAGGGTATATCCTGCTTTTAATAACGCTTCg < 2:129782/251‑1 (MQ=255) cacGACTTATGGCTGGTAATACCCAGACGTGCGATCCATTTTTCCGGAGCAAATCCACCAGCCTTATGGATA‑GTGGCGCTTCTTACCGCAACCATCTTCCTCTCCTTTAGGAACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAGTCCAGACGTCCCGTGTGTGGGAACATAGCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACTCTTCg < 2:281316/251‑1 (MQ=255) gtggctggAAATACACAGACTTGTGAGCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑AACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGAACCATCGATTCCAGATGTCCCGTGAGTGGGAACATATCAAACATCCCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCg > 2:217704/2‑251 (MQ=255) accccatccTTGCGATCCATTTTTCGGTACCAAATTCGCAAGCTTTATTGCAATGTG‑CACTTCTTACCGCACCTATCTTCCCCCCCTGTAGGGACCTGCCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGTGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGGCGCGCAATGGTATATCCTGCTTTTAATACCGATTCGTTATCCCGAGCCAGCGTTg < 2:302188/243‑1 (MQ=255) aCCCAGACTTGCGATACATTTTTACGGAGCAAATTCAACAGCCTTATCGCTATGTG‑CACTTCTTACCGCAACCATCGTCCTCTACTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCAAACATCCACATTCGCGCAATGGAATATCCTGCTTTTAATAACGCTTCGCTATcccactccagcgtttc > 2:175105/1‑238 (MQ=255) actggCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGAAACCAGCGGCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGtt < 1:40989/247‑1 (MQ=255) gctCCATTCTGCCGGATCAAATCCACCAGCCTTTGTGACAGGTC‑CACTTCTTACCGCACCCATCGTCCTCACCTTTAGGGACCAGGCTTGCCGAAATCGGCTAAGCGCAACGATTTAACGCGCGAGAAAAGTTCCATCGATTCCATATGTCCCGTGTGTTGGAACATAGCCAGCAACGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCGATTCCGAGCTAACGGTTCAGGGTTTCAGGa < 2:226811/249‑1 (MQ=255) tCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCCACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACt > 2:316243/1‑251 (MQ=255) ggATCAAATTCCCCAGCCTTATTTATATGTG‑CACTTCTTCCCGCAACCATCGTCCTCCCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACCTCGCCAGTCGCGCAATGGTATAACCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACtata < 2:270959/251‑1 (MQ=255) ccAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTcc > 2:76072/1‑251 (MQ=255) ccTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGc > 1:10372/1‑205 (MQ=255) ccTTATTGATAGGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATAGCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGc < 2:10372/205‑1 (MQ=255) tGATATGTG‑CACTTCTTACCGCAACAATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATAGGCAAATCGCAACTATATAACGCGCAAGCAAAGTACCATCGATTACAGATGTCCAGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCACTGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAATCTACACGAATAGGTTCCAGGTTtata > 1:85428/1‑250 (MQ=255) gATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTACTTTAGGGACCAGACATGCCGAACTCGGCCAATCGCCACTATTTAACGCGCGCGAAAAGTCCCATAGACTCCCGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGAATATCCTGCTTTTAATAACGCTTCGCTATCCCGCGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAAt > 1:24053/1‑251 (MQ=255) atatGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGTATACATAAACTATACGAATAGGTTCCAGTTTTATAAtt > 2:101619/1‑251 (MQ=255) tg‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGACAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGAt > 2:14556/1‑210 (MQ=255) tg‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGAt < 1:14556/210‑1 (MQ=255) cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCACATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCa > 2:329139/1‑183 (MQ=255) cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCa < 1:329139/183‑1 (MQ=255) tACCGCAACCATCGTCCGCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAac < 1:316243/248‑1 (MQ=255) cgttgaGGGCCCAGCCCTGCCGAAATCGGCAAAGCGCAACTATTTAACGCGCGAGAAAAGGACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACAGCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCg < 2:307523/246‑1 (MQ=255) gggACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTGCCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCa < 2:133881/251‑1 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAACATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAaca < 1:76072/251‑1 (MQ=255) | GTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTG‑CACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACA > NC_000913/2913519‑2913922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |