Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,903,727 G→A intergenic (+353/+1020) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,7270GA91.7% 29.0 / ‑6.3 13intergenic (+353/+1020)ygcE/queEputative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1/10);  minor base T (0/1);  total (2/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GGTTTATCCCCGCTGATGCAGGGAACATAATAAAAACTTTAACTAGTTCGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATT‑AATTCAATATTAAAAAAATAATAGATTAAAA‑TTTCTTAACGATTTAAGAATCATACAAATAACACTTTGATTAAATTTAATTTTTTGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGA  >  NC_000913/2903482‑2903930
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                             
ggtgtATCCCCGCTGATGCAGGGAACATAACAAAAACTTTAACGAGTTCGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTTGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCAGTTATGTAAAACCACTCATTAGCCTTAAAACAAAGACAAACTT‑CCAAATtta                                                                                                                                                                                                          <  2:195505/247‑1 (MQ=255)
   ttATCCCCGCTGATGCAGGGAACATAATAAAAACTTTAACGAGTTCGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAAGTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATATAGGCGGTTATGTAAAACCACTCATTAGCCGCAAAACAAAGACAAACTT‑CCAAACttatt                                                                                                                                                                                                        <  2:363425/250‑1 (MQ=255)
                            aataaAAACTTTAACTAGTTCGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACAAGCTTa                                                                                                                                                                               <  1:26675/250‑1 (MQ=255)
                                            atttCGGTTTATCCCTGGTGTTACAAGGAATATTAACTAGACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATAGCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGTCGGTTATGTATAGCCACTTATTAGCCACAAAACAAAGATAAACTT‑CCAAATTTATTACTTATCCAATGTATAACAAGTGTAAAAAATATAGTGTTa                                                                                                                                                                <  2:232253/247‑1 (MQ=255)
                                                cGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAATACAAACTT‑CCAAATTTATTCCTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTa                                                                                                                                                           <  1:184343/250‑1 (MQ=255)
                                                         ccTGTTTTTACAAGGAATAGTAACTACACTCGATGTCTTTAGAATAAAAGACACTACGAATCACACTTTATCAAATTTCACTTAAAATATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACATCGTATAGAGGCGGTTATGTAAAACCACTCAGTAGCCTCAAAACAAAGACAAACGT‑CCATAGTTAATACTTAACCAATGAATGACAAGCATAAAAAATAGACTGTTATTCTAAAGAAAAAAt                                                                                                                                                 <  2:97003/251‑1 (MQ=255)
                                                                             tAACTCCACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAACCCCCCACTAGCCTCAAAAAAAAAAAAAAAAT‑TCAAAAATATTACTTAACCAATGAATAAAAAGCATAAAAAATATACTGATATTTTAAAAAAAAAAATAACatacaataattaaatt                                                                                                                             >  1:297694/1‑236 (MQ=255)
                                                                                           gTCTTTAGAATAAAAGACAATACGAATCTCACTTTATCAATTTTCACTTAAAGTATGAAATGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAAGTAACAGGCATT‑AATTCAATATTAAAAAAataata                                                                                                              <  2:297694/251‑1 (MQ=255)
                                                                                                                                                                     cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATT‑AATTCAATATTAAAAAAATAATAGATTAAAA‑TTTCTTATCGATTTAAGAATCATACAAATAACACGTTGATTAAATTTAATTTTTTGTATCGATAAt                                   <  1:282027/251‑1 (MQ=255)
                                                                                                                                                                          gAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACCCATTTGCCTCAAAAAAAAAAAAAACTTTCCA‑ATTTTTTACTTTTACAAAGAATAACAAAATTAAAAAATATATTGTTATTTTAAAAAAAAAAATAAAAAGCATTTAATT‑AATATTTAAAAAAAAAAAGATTAAAAATTTTTTAA‑GATTTTAAAATCATACAAAAAACACATTTATTTAATTTTATTTTTTTTATTGATAAttttg                               >  1:301700/1‑247 (MQ=255)
                                                                                                                                                                                           cGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACa                                                                                                                                                                                     <  1:204197/85‑1 (MQ=255)
                                                                                                                                                                                              ataGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATT‑AATTCAATATTAAAAAAATAATAGATTAAAA‑TTTCTTAACGATTTAAGAATCATACAAATAACACTTTGATTAAATTTAATTTTTTGTATCGATAATTGTGAACCTCTCTGGCATGGAGaa           <  2:11484/250‑1 (MQ=255)
                                                                                                                                                                                                      ggTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAAATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATT‑AAATCAATATTAAAAAAAGAATAGATTAAAA‑TTTCTTAACGATTTAAGAATCATACAAATAACACTTTGATTAAATTTAAGTTTTTGTATCGAGAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGa  <  1:16000/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                             
GGTTTATCCCCGCTGATGCAGGGAACATAATAAAAACTTTAACTAGTTCGGTTTATCCCTGTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTT‑CCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATT‑AATTCAATATTAAAAAAATAATAGATTAAAA‑TTTCTTAACGATTTAAGAATCATACAAATAACACTTTGATTAAATTTAATTTTTTGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGA  >  NC_000913/2903482‑2903930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑