Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,434	G→A	intergenic (+816/+135)	iap → / ← cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,434	0	G	A	100.0%	45.8 / NA	17	intergenic (+816/+135)	iap/cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

GGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAGCGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCC  >  NC_000913/2878210‑2878675                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ggggAACTCGCGCCCGCTCAGAAATTCCAGACCCGATCCACACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTCCAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGTCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGaa                                                                                                                                                                                                                         <  2:238794/251‑1 (MQ=255)     aaCTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGTGGAActct                                                                                                                                                                                                                     <  2:296725/251‑1 (MQ=255)                gCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGCGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTACGTGTGCGTATCGCCGCGCGTCTGCGTACACGGTTTAACCCCGATCGCACGGGGCACTCTCTaaatgcat                                                                                                                                                                                                           >  2:120067/1‑245 (MQ=255)                                                          ggCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCCCCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATc                                                                                                                                                                                                                                          >  2:192764/1‑177 (MQ=255)                                                          ggCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATc                                                                                                                                                                                                                                          <  1:192764/177‑1 (MQ=255)                                                                                      tACAACCGACAGGGAGCCCGGTTAATCCCCGCAGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCCCCGCCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTACCATTTCCttattatt                                                                                                                                    >  1:302835/1‑251 (MQ=255)                                                                                          aCCGACAGGGAGCCCGGATTATCCCCGCTAGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTAGGCTGCGGTTTATCCCCGCTGGCGCGGGGAAATCTGAGTGAGCGTATCGCCGCGCGTCTGCGGAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATAAATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTaa                                                                                                                                  >  1:80831/1‑249 (MQ=255)                                                                                                                ccccGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACATTAATGTAACATTTCCTTATTATTAAAGATCCGCTACTTCTTTGTTTTc                                                                                                           >  2:249069/1‑250 (MQ=255)                                                                                                                    gCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAAc                                                                                                       >  2:86350/1‑250 (MQ=255)                                                                                                                                                        ttGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCAGCCAAATc                                                                   <  1:100812/250‑1 (MQ=255)                                                                                                                                                                ggCTGCGGTTTATCCCCTCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCCCGTCTGCGAAAACAGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATGAAAGATCCGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCGTAAACCATCCAAAGCTACCgggtt                                                          <  2:202173/251‑3 (MQ=255)                                                                                                                                                                                 gCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGAAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCaa                                         <  1:83047/251‑1 (MQ=255)                                                                                                                                                                                      cgcgGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTc                                    >  1:180661/1‑251 (MQ=255)                                                                                                                                                                                                 ctGCGTGAGCGTAGCGCCGCGCGTCTGCGAAAACGGTATATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCGGGAAc                              <  1:240710/246‑1 (MQ=255)                                                                                                                                                                                                                 ccgcGCGTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTc         >  2:217465/1‑251 (MQ=255)                                                                                                                                                                                                                  cgcgcgTCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCg        >  2:180088/1‑251 (MQ=255)                                                                                                                                                                                                                        tCTGCGAAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGccc  >  1:361575/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  GGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAGCGGTTTATCCCCGCTGGCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCC  >  NC_000913/2878210‑2878675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑