Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,873,316	T→C	T8A (ACA→GCA)	ygbE ←	DUF3561 domain‑containing inner membrane protein YgbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,873,316	0	T	C	100.0%	120.9 / NA	34	T8A (ACA→GCA)	ygbE	DUF3561 domain‑containing inner membrane protein YgbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/18);  total (16/18)

AACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGTAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCGGCACGCGCTTTC  >  NC_000913/2873074‑2873546                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       aaCAATTCTGGAGCGGAGCTCTCCGTCCATCAAAGAATGCAGCGCAAACCCCACCACTACCGCGACGGGCAGCAGCGACAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTAt                                                                                                                                                                                                                                <  2:117764/251‑1 (MQ=255)                            cATCAATGAATGCAGCGCAATCCCCACCACTACCTCGACGGGCATCAGCGCCAGAAAGAAAGGTCAGGTGTAGATAAAGAAGAACATCGTTGTAGAGCCATAAATCAACAGCGGCATCGCCAGCTCAAATAACCAGGAGATAAAACCGACCACGGTACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGTTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTa                                                                                                                                                                                                     <  2:130393/251‑1 (MQ=255)                                             cAATCCCCACCACTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGc                                                                                                                                                                                     >  1:83958/1‑250 (MQ=255)                                              aaTCCCCACCACTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAAAAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGGGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGACGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCAt                                                                                                                                                                                   >  2:193689/1‑251 (MQ=255)                                                       cacTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCg                                                                                                                                                                          <  1:169412/251‑1 (MQ=255)                                                        acTACCGCGCAGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGCACAGCGTGTTAGAGCCATAAATCAACATCGGCACCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCgg                                                                                                                                                                         >  2:346707/1‑251 (MQ=255)                                                        acTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATCTTTGATCCTGTTACTTTTCCGCACCGGGCATGGAAACCCgg                                                                                                                                                                         >  2:33992/1‑251 (MQ=255)                                                           aCCGCGACGGGCATCAGCGCCCGAAAGAAAGGCCAGGAGAAGATAAAGAAGAAAAGCGCGCTAGAGCCATAAATCACCATCGGCATCGACAGAGCCAATAAACCGGAGATAAAACCGACCAAGGCACCAGGCAGTGCCCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTAAGCATATTTGATACTGTTACTTTGACGAACCGGGCATGGAAACCCggtgg                                                                                                                                                                      >  2:203544/1‑251 (MQ=255)                                                                             gcCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCAGCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACGTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGataa                                                                                                                                                    <  1:203544/251‑1 (MQ=255)                                                                                      aaagGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCt                                                                                                                                           <  2:88646/251‑1 (MQ=255)                                                                                      aaagGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCt                                                                                                                                           >  2:324138/1‑251 (MQ=255)                                                                                        acgCCAGGTGTAGACAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAATCCGACCACGGCACCAGGCAGGGACCATGTGGTGTCTTCATCCTCAGTAAGGCTGTCGTTATTCGTTAGTGCAATGTTAGGGCTATGACGCATATTGGATCCTGTTACTTTGACTAACCGGGCATGGAAACCCGGTGGTGTCTCAGGAGCGGATAATAGCGTTCTGt                                                                                                                                         <  2:242146/249‑1 (MQ=255)                                                                                                  tAGATAAAGAAGAACAGCGTGTTAGAGCCATAACTCACCATCGGCATCGCCAGCGCCAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAAc                                                                                                                                   >  1:206572/1‑247 (MQ=255)                                                                                                   aGATAAAGGAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACAAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCCTATTTGATCCTGTTACTTTGACGAACCGGGCATGGACACCCGGTGGGGTCTCAGGTTCTGATAATATCGt                                                                                                                                              >  2:267337/1‑235 (MQ=255)                                                                                                   aGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCACCAGCGCAAATAACCAGGAGATAAATCCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAAGGTTATGGCTATTACGCATATGTGATCCTGTTACTTTGACGATCCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGt                                                                                                                                              <  1:267337/235‑1 (MQ=255)                                                                                                     aTAAAGAAGAACAGCGTATTAGAGCCATAAAGCAACATCTGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGTCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGGAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGGGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCta                                                                                                                            <  1:211263/251‑1 (MQ=255)                                                                                                                           agCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGCTCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGt                                                                                                      >  2:154746/1‑251 (MQ=255)                                                                                                                              cATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTAc                                                                                                   <  2:137875/251‑1 (MQ=255)                                                                                                                                   aTCAACAGCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTGGTTACTAAtt                                                                                              <  2:85525/251‑1 (MQ=255)                                                                                                                                          ccGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCAcc                                                                                       <  1:233120/250‑1 (MQ=255)                                                                                                                                                        gcAAATAACCAGGAGATGAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAAttt                                                                         <  2:197489/251‑1 (MQ=255)                                                                                                                                                                 cAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAAtt                                                                                              >  2:264642/1‑221 (MQ=255)                                                                                                                                                                 cAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAAtt                                                                                              <  1:264642/221‑1 (MQ=255)                                                                                                                                                                              cGACCACGGCAACAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGt                                                    >  2:317611/1‑250 (MQ=255)                                                                                                                                                                                      gCACCAGGCAGTTACCATGTGGTTTCTTCATCCTCAGTAAGGCTTTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCTGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAAt                                           <  2:229235/251‑1 (MQ=255)                                                                                                                                                                                                    ccATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAg                             <  1:163769/251‑1 (MQ=255)                                                                                                                                                                                                          ggTTTCTTCATCCTAAGTAAGGCTGTCGTTATTTGTTAGGGCAATGTTATGGCTATTACGCATAGTTGAGCCTGTTAATTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTgcgc                       <  2:275172/251‑1 (MQ=255)                                                                                                                                                                                                               cttcATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTc                  >  1:202940/1‑251 (MQ=255)                                                                                                                                                                                                                   aTCCTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCg              >  1:294628/1‑251 (MQ=255)                                                                                                                                                                                                                      cTCAGTAAGGCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCGGc            >  2:57257/1‑250 (MQ=255)                                                                                                                                                                                                                       tCAGTAAGGCTGTCGTTATTTGTGAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGCTTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCGGCAc          >  2:267548/1‑251 (MQ=255)                                                                                                                                                                                                                               gCTGTCGTTATTTGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTGCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCGGCACGCGCTTTc  <  1:324138/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTGATTGtt                                                                                           <  1:99764/151‑1 (MQ=255)                                                                                                                                                                                                                                          ttGTTAGTGCAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGtt                                                                                           >  2:99764/1‑151 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       AACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGACGGGCATCAGCGCCAGAAAGAAAGGCCAGGTGTAGATAAAGAAGAACAGCGTGTTAGAGCCATAAATCAACATCGGCATCGCCAGCGCAAATAACCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCATCCTCAGTAAGGCTGTCGTTATTTGTTAGTGTAATGTTATGGCTATTACGCATATTTGATCCTGTTACTTTGACGAACCGGGCATGGAAACCCGGTGGTGTCTCAGGATCTGATAATATCGTTCTGTCTCAACAGATCTAATAATTGCTGTACCAAATTTGTTACTAATTGTTCACCATTGAGATGAATTTCTGCCGATTCAGGCGCTTCGTAAACGGAATCTATTCCCGTAAAGTTGCGCAGTTCACCGGCACGCGCTTTC  >  NC_000913/2873074‑2873546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑