Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,648,617	T→C	D1224G (GAT→GGT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,648,617	0	T	C	100.0%	248.4 / NA	74	D1224G (GAT→GGT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

GGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCATCCGCCGGAATCGCCCATGTC  >  NC_000913/2648379‑2648862                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ggCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCAc                                                                                                                                                                                                                                           >  1:355522/1‑251 (MQ=255)    tttGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGACGATACCGACCGATGCACGGc                                                                                                                                                                                                                                        >  2:246436/1‑251 (MQ=255)     ttGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCg                                                                                                                                                                                                                                       >  1:175458/1‑251 (MQ=255)     tgtggaTTAGCCGCGTACGGGATCGCCATCACGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATGACCCGGGTTAATGTGGTCTGT‑CGGCACGCGGTACCCCGGGTCGCCGGCGCGGACCAGGAAATCCACCCCGTAAGCCGTCAGCCAGTACTCTTCGTCCCCTTTTTGATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACgg                                                                                                                                                                                                                                         <  2:143624/245‑1 (MQ=255)       gagaTTATCCGCGTACGGGATCGACAGCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTCCCCCGGTTAATGGCGTCTGC‑CGGCCCGCGGTACCCCTGTTCGCCTGCGCGGCCCAGGAAATCCACCCCGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTACCCCACAGCGAAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGtt                                                                                                                                                                                                                                     <  2:175458/251‑1 (MQ=255)               ccgcgttggggcgcGACACCATGCCGGGATTTTGTAAATAGCGCAGAAGACGCTCATTACCCCGGTTAAGGGCGTATGT‑GGGCACGCTGTAACCCTTTTCGCCGGCGCGGACCAGGAAATCCATCACGTAAGCGTTCAGCCAGTACTCTTCGTCCCCGTTTTAATCCCACAGCGTAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCAGGGCGTTTCtcatca                                                                                                                                                                                                                             <  2:157412/238‑1 (MQ=255)                cgcgTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCTCCGCAGACGCTCATTTCCCCGTTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCTTGCGCGGACCAGTAAACCCACCACGTAATCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCAc                                                                                                                                                                                                                            <  1:239591/251‑1 (MQ=255)                   gTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGt                                                                                                                                                                                                                         >  1:100058/1‑251 (MQ=255)                    gccggcatctctaccttGCCCGGATCCTGTAAATAGCGCAGCAGCCCCTCATTTCCCCGGTGAAGCGCGT‑TGTACGCCCCGCTGTACCCCTGTTCGCCGACGCGGCCCAGGAAACCCTCCCCGTAAGCCGTCCGCCAGTTCTCTTCTCCCCCGTTTTTATCCCACATCTCAATG‑CCGCCGTTATCTCGTTGCATTTGCAGCAAACGGGAAATTCCGATACCGACCGATGCACGGCGTTTCTCATCACTGt                                                                                                                                                                                                                         <  2:28043/234‑1 (MQ=255)                        ggATCTACATCATGCCCGGATCTTGTAAAGAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTACCCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCt                                                                                                                                                                                                                    <  1:156038/251‑1 (MQ=255)                         gATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCtt                                                                                                                                                                                                                   >  1:174210/1‑251 (MQ=255)                                        cggtctttGTAAATCGCCCAGCAGGCGCCCATTCCCCGGGTTATGGGCGTCTGC‑CGCCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAAGCCTTCACGTAAGCCGTCAGCCATTACTCTTCGTCACCTTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCt                                                                                                                                                                                                    <  2:10732/245‑1 (MQ=255)                                         ggATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTg                                                                                                                                                                                                   >  1:276729/1‑251 (MQ=255)                                               tGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTTC‑CGGCCCGCTGTACCCCTTTTCGCCGGCGCGGCCCAGGAAATCCATCACGTAAGCCGTCGGCCAGTACTCTTCGTCCCCGTTTTTACCCCCCAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCa                                                                                                                                                                                                 <  2:159449/247‑1 (MQ=255)                                                            ccagACGCTCATTCCCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCATTGGta                                                                                                                                                                                <  1:102300/250‑1 (MQ=255)                                                             cagACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGta                                                                                                                                                                                >  2:102300/1‑250 (MQ=255)                                                              agACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGtat                                                                                                                                                                               >  1:190427/1‑250 (MQ=255)                                                                     cATTACCCCGGTTAATGGTGTTGGT‑CGGCACGCGGTAACCTGGTCCGCCGGCGCGGACCAGGAAATCCATCCCGTAAGCCGTCAGCCAGTACGCTGCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGAc                                                                                                                                                                       <  2:284764/251‑1 (MQ=255)                                                                       ttACCCTGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACgg                                                                                                                                                                     >  1:98612/1‑251 (MQ=255)                                                                       ttACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACgg                                                                                                                                                                     >  2:325981/1‑251 (MQ=255)                                                                        tACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGGGACgg                                                                                                                                                                     >  1:339905/1‑250 (MQ=255)                                                                         cccccGGTTAAGGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCGGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTGCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACGGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGa                                                                                                                                                                    <  1:333823/249‑1 (MQ=255)                                                                                  aagggCGTCTGT‑CGGCACGCTGTAACCCTGTCCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCg                                                                                                                                                           <  1:325981/247‑1 (MQ=255)                                                                                  aaTGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGc                                                                                                                                                          >  1:143118/1‑251 (MQ=255)                                                                                  aaTGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGc                                                                                                                                                          >  1:76213/1‑251 (MQ=255)                                                                                    tGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTg                                                                                                                                                        >  2:232934/1‑251 (MQ=255)                                                                                     ggCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTgg                                                                                                                                                       >  2:95006/1‑251 (MQ=255)                                                                                      gCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGc                                                                                                                                                      >  1:183863/1‑251 (MQ=255)                                                                                               cGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTCCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCt                                                                                                                                              <  1:51967/251‑1 (MQ=255)                                                                                                        gtaccccTGTTCGCCGGCGCGGACCAGGAAATCCACCCCGTAAGCCGTCAGCCAGTCCTCTTCGTCCCCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACa                                                                                                                                       <  2:277314/245‑1 (MQ=255)                                                                                                        gTAACCCTGTTCGCCGGCGCGGACCAGGAAATCCATCCCGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCCCAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAg                                                                                                                                      <  1:144022/250‑1 (MQ=255)                                                                                                         tAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCCCCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGcc                                                                                                                                    >  1:174142/1‑251 (MQ=255)                                                                                                           aCCCTGTTCGCCTGCGCGGCCCAGGAAATCCACCTCGTAACCCTTCAGCCAGTACTCTCCGTCCCCGTTTTAATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGcc                                                                                                                                                                                                                     <  1:352588/168‑1 (MQ=255)                                                                                                           aCCCTGTTCGCCTGCGCGGAGCAGGAAATCCATCACGTAAGACGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGcc                                                                                                                                                                                                                     >  2:352588/1‑168 (MQ=255)                                                                                                            cccTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGAGGTTTGCTCAAGACAGCCgg                                                                                                                                  >  1:1495/1‑249 (MQ=255)                                                                                                              cggtccGCCGGCGCGGCCCAGAAAACCCACCCCGTAAGCCGCCAGCCAGTACTCTCCGCCACCGTTTTTACCCCACAGCGAAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACg                                                                                                                               <  2:276729/246‑1 (MQ=255)                                                                                                                ttccGCCTGCGCGGACCAGGAAATCCATCCCGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTGGCTCAAGACAGCCGTACg                                                                                                                               <  1:136732/246‑1 (MQ=255)                                                                                                                gTTCGCCGGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGGTTGCTCACGACAGCCGTACgg                                                                                                                              >  2:22722/1‑250 (MQ=255)                                                                                                                    gCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGt                                                                                                                                                   <  1:284054/225‑1 (MQ=255)                                                                                                                    gCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGt                                                                                                                                                   >  2:284054/1‑225 (MQ=255)                                                                                                                     ccTGCGCGGACCAGGAAATCCATCCCGTAAGCCGTCAGCCAGTTCTCTTCGTCACCGTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGc                                                                                                                        <  1:45527/251‑1 (MQ=255)                                                                                                                            ggACCAGAAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAAc                                                                                                                 >  1:76356/1‑251 (MQ=255)                                                                                                                              aCCAGGCAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTATCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAActc                                                                                                               >  1:246051/1‑251 (MQ=255)                                                                                                                               ccagaaaatcacccACGTAAGCCGCCACCCAGTCTTCTTCTTCACCGTTTTTACCCCACAGCAAAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTGCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAActct                                                                                                              <  2:283918/238‑1 (MQ=255)                                                                                                                                  ggAAACCCATCACGTAAGGCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGAAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTg                                                                                                           <  1:2252/251‑1 (MQ=255)                                                                                                                                  gaaaatccctcccggaagcccccatccAGTACTCTGCGTCCCCGTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCTTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTTCTCAAGACAGCCGTACGGATACGCTTTTAACTCGTTg                                                                                                           <  1:2249/226‑1 (MQ=255)                                                                                                                                    aaacccATCACGTAAGCCGTCAGCCAGTACTCTCCGTCACCGTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCGGCAGTTGGGCTGCGTTGGTATAAAGTGACg                                                                                                                                                                      <  2:218857/186‑1 (MQ=255)                                                                                                                                    aaaTCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACg                                                                                                                                                                      >  1:218857/1‑190 (MQ=255)                                                                                                                                    aaaTCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATAAGCTTTTCACTCTTTGat                                                                                                         >  1:266659/1‑251 (MQ=255)                                                                                                                                            cacaaaggCCGTCAGCAAGTACTTTTCGTCACCGTTTTTACCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTg                                                                                                 <  2:143118/244‑1 (MQ=255)                                                                                                                                                  aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGTAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGt                                                                                           >  2:181215/1‑251 (MQ=255)                                                                                                                                                  aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGt                                                                                           >  1:124839/1‑251 (MQ=255)                                                                                                                                                            cAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCggtgg                                                                                 >  2:181094/1‑251 (MQ=255)                                                                                                                                                                       gTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGGTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGGTCAGCGGTGGTTTGCCGCTc                                                                       >  1:106732/1‑250 (MQ=255)                                                                                                                                                                        tccgcttttgtttCCCACAGC‑CAAAGACCGCCGTTATACCGTGGCATTTGCAGGAAACGGGAAATGCCGTTACCGACCGATGCACGGCGTTTCTCCTCACTGTCGCCTTTGATGCCCAACTCCTGCAGTTGGGTTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGACGTACGGATACTCTTTTAACGCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTca                                                                      <  2:184855/238‑1 (MQ=255)                                                                                                                                                                             gTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTcaaaaac                                                                 >  1:130748/1‑250 (MQ=255)                                                                                                                                                                                tttATCGCACAGCGCAAAG‑CCGCCCTTATCACGTTGCATTTGCAGCACCCGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTAATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAACGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGGCCGGGTACGCTTTTACCTCTTTGATATCACGTGCGATGTTCAGCGGTGGTTTGACGCTcacaaccaagt                                                             >  2:318605/1‑242 (MQ=255)                                                                                                                                                                                tttACCCCACAGCACAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAAt                                                              <  2:98612/250‑1 (MQ=255)                                                                                                                                                                                  tATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTg                                                            <  1:293452/250‑1 (MQ=255)                                                                                                                                                                                    ccccACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGccc                                                         <  1:69768/250‑1 (MQ=255)                                                                                                                                                                                      ccACAGCGCAAAG‑CCGCCGGTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGGCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCtt                                                       <  2:31236/251‑1 (MQ=255)                                                                                                                                                                                       cacaGCGAAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTc                                                      <  1:181094/251‑1 (MQ=255)                                                                                                                                                                                                           tATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGaa                                    <  2:130748/250‑1 (MQ=255)                                                                                                                                                                                                           tATCACGTTGCATGTGCAGCAAACGGGAAATGCCGATACCGCCCGAGGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTAGAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCGTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGaa                                    <  2:87651/250‑1 (MQ=255)                                                                                                                                                                                                              caccttGCATTTGCAGCAATCAGGCAATGCCGATACCGACAGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGATCACGACAGCCGTACGGATACGCTTTTAACTCTTTGATATACAGTGCGATGTTCAGCGGTGGTTTGCAGCTCCACAACAATTGCCCTTCCCGCGTAACAGGCGGGCAGttt                                >  2:358560/5‑251 (MQ=255)                                                                                                                                                                                                                   tGCATTTGCAGCAAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCa                            >  1:90852/1‑250 (MQ=255)                                                                                                                                                                                                                          gcagcaAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAatccatcc                    >  1:48264/1‑251 (MQ=255)                                                                                                                                                                                                                          gcagcaAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAatccatcc                    <  1:225546/251‑1 (MQ=255)                                                                                                                                                                                                                          gcagcaAACGGGAAATGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAatccatc                     <  1:135677/250‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGGAAATGCCGATCCCGCCCGATGCACGGCGTGTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCTTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACGCTTTGATATAACGTGCGATGTTCAGCGGTGTTTTGCCGCTCAACAACAATTGCCCTGCCAGCGTAACAGGCGAGAAGGTTTGCAATCCATCCGCCGGac             <  2:157034/251‑2 (MQ=255)                                                                                                                                                                                                                                    ggAAAAGCCGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCATCCGCCGGAATCg          >  1:247454/1‑251 (MQ=255)                                                                                                                                                                                                                                     gAAATGCCGATACCGACCGATGCACGGCGTTTCCCATCACTGTCGACTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCACGACAGCCGCACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCATCCGCCGGAATCGc         >  1:362312/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaTGCCGATGCCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCGTTGATATAACGTGCGATGTTCAGCGGGGGTTTGCCGCTCAACAACAATTGCCCGTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCACCCGCCGGAATCGccc       <  2:24272/251‑1 (MQ=255)                                                                                                                                                                                                                                            cGATACCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCATCCGCCGGAATCGCCCATGtc  <  1:37390/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGT‑CGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAG‑CCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGACCGATGCACGGCGTTTCTCATCACTGTCGCCTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAATTGCCCTTCCAGCGTAACAGGCGAGAAGTTTTGCAATCCATCCGCCGGAATCGCCCATGTC  >  NC_000913/2648379‑2648862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑