Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,629,583	T→G	intergenic (+102/‑209)	yfgG → / → yfgH	nickel/cobalt stress response protein YfgG/lipoprotein YfgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,629,583	0	T	G	100.0%	81.7 / NA	26	intergenic (+102/‑209)	yfgG/yfgH	nickel/cobalt stress response protein YfgG/lipoprotein YfgH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/13);  total (13/13)

CGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAA  >  NC_000913/2629349‑2629808                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                  cGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCct                                                                                                                                                                                                                    <  2:167274/250‑1 (MQ=255) cGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGGCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCGCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACAGAACGGCACTTCGCTGTCGGATGCTTTTGCTGGTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTGATccc                                                                                                                                                                                                                    <  2:20798/250‑2 (MQ=255)         taatcagctttatattactatttacccGTTTTATCAAGTCGTCCGACGGAGCTTGGCAGCAACATCAAAGCCCAAAAGAAGATCAGCAATCCACACTCTCCATAGAATCACCGATACAACGTTAGCGGTTACCTTCTACCCTTTCACCGAACATAACGGCACTTCGCTGTCGGATGCTTTAGCTGTTTGGGATTATCAAAACGGCGGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGt                                                                                                                                                                                                           >  2:357323/26‑251 (MQ=255)           atcaGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGttt                                                                                                                                                                                                         >  2:202522/1‑251 (MQ=255)             caGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTa                                                                                                                                                                                                        <  2:113366/250‑1 (MQ=255)             caGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTAt                                                                                                                                                                                                       <  2:250844/251‑1 (MQ=255)                    aTCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGttt                                                                                                                                                                                                >  2:108522/1‑251 (MQ=255)                           tcttTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCt                                                                                                                                                                                            <  1:202522/248‑1 (MQ=255)                           tcttTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTg                                                                                                                                                                                           <  1:17350/249‑1 (MQ=255)                                        tATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGTAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATTAATATaa                                                                                                                                                                             >  2:192529/1‑250 (MQ=255)                                                cgtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAATCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAatat                                                                                                                                                                    <  2:159015/251‑1 (MQ=255)                                                   ccgtcGGTGCCTGGCAGCACCATAACAGCAAAAAAGAAGCTCAGCAATCCACACGCTCCGTCGAATCACAGGTATAACGTTAGCGTGTACCTGCTCCTCTGTCATAGAACATAACGTCACTTCGCTGTCGGATGCTTTTGCTGTTTTGTATTATCAAGTCGGCATATATTCTTTCATCTTTAATGTTACATCTTTATCCTGATTGATGTTTATCGTGTTTGGGTGCGAAATAAAGATAAAAGTAatatat                                                                                                                                                                  <  2:361371/250‑1 (MQ=255)                                                     gtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGTATTATCAAAGCGGCAGATATTCTTGCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATAtg                                                                                                                                                               <  1:300079/251‑1 (MQ=255)                                                         gTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCAGTCGAATCACCGGTACAACGTTAGCGGATACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATAtgttgt                                                                                                                                                           >  2:223121/1‑251 (MQ=255)                                                                          cAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTtata                                                                                                                                          <  1:147493/251‑1 (MQ=255)                                                                                 aaaaaGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAattatt                                                                                                                                   >  1:231527/1‑251 (MQ=255)                                                                                               cAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAAttt                                                                                                                     >  1:349429/1‑251 (MQ=255)                                                                                                                              aaCGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCa                                                                                       >  2:88793/1‑250 (MQ=255)                                                                                                                               aCGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAAt                                                                                     <  1:258040/251‑1 (MQ=255)                                                                                                                                      cGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACAttt                                                                               >  2:31515/1‑250 (MQ=255)                                                                                                                                                                cATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATg                                                    >  1:111218/1‑251 (MQ=255)                                                                                                                                                                             cGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGt                                       >  1:46160/1‑251 (MQ=255)                                                                                                                                                                                tGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAAttt                                                                                                                     <  2:173975/170‑1 (MQ=255)                                                                                                                                                                                tGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAAttt                                                                                                                     >  1:173975/1‑170 (MQ=255)                                                                                                                                                                                           tttGCTGTTGGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATGTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTTATCTATCCCTTTATACGCAATTCATTTACTTTCCTCTTTTGATTATCTTAAATGTCTTATTTTTCGTAATGTGCATAACa                          <  2:111557/250‑1 (MQ=255)                                                                                                                                                                                                                   ggCAGATATTCTTTCATCTTAAATGTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTaaaaa  >  1:60443/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                  CGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAAATATAAAATTAATATATATGTTGTAATGATATATTTTTATAAATTATTCCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAA  >  NC_000913/2629349‑2629808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑