Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,292	G→A	R94H (CGT→CAT)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,292	0	G	A	98.2%	187.7 / NA	58	R94H (CGT→CAT)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (28/28);  minor base C (1/0);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCT  >  NC_000913/350047‑350532                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCg                                                                                                                                                                                                                                             >  2:332016/1‑251 (MQ=255)   tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTCCCGGCTAACGTGCATACCGTg                                                                                                                                                                                                                                           >  1:349863/1‑251 (MQ=255)                          gCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTc                                                                                                                                                                                                                    <  1:255724/251‑1 (MQ=255)                          gCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGGGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTc                                                                                                                                                                                                                    <  1:324432/251‑1 (MQ=255)                              tttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCAc                                                                                                                                                                                                                >  1:267617/1‑251 (MQ=255)                                   ggCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCgat                                                                                                                                                                                                           >  1:179109/1‑251 (MQ=255)                                     cGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCgatgg                                                                                                                                                                                                         >  1:152756/1‑251 (MQ=255)                                               ggCAATACGGCGCTCTGCACCATGGGTAAAAGTGGCAATGAACTGCATTCCCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGGTCCACGGCACACTGCCGACCCGTGACGAACTCGCCGATTACAAAAAGAACCTGAAAGCCCTGGGCGGTTTACCGGCTAACGGGCGTACCGTGCTGGAAGCCTTGCCGGCGGAGTCGCAACCGAAGGATGTTATg                                                                                                                                                                                                 >  2:298883/1‑249 (MQ=255)                                                  aaTACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCAc                                                                                                                                                                                            >  1:98652/1‑251 (MQ=255)                                                            ccggaaCCGTGGGTAAAAGTGGCAATGACCTGCATGACCGCGGCTACGATATTCTTAATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTATCGTGCATACCGTGCTGGAAGCCGTACCGGCGGCGTCGCCCCCGATGGATGTTATGCGCACCGGTGTTTcc                                                                                                                                                                                  <  2:224059/246‑1 (MQ=255)                                                                  ccGTTGGTAAAAGTGGCAATGCCCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATGTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTAGAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTTGGAGCTTTACCGGCGGCGTCGTACCCGATTGATGTTATGCGCTCCTGTGTTTCCGCGCTc                                                                                                                                                                            <  2:163340/251‑1 (MQ=255)                                                                         tAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGACACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCtagg                                                                                                                                                                     >  2:176314/1‑248 (MQ=255)                                                                         tAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCt                                                                                                                                                                        <  1:176314/248‑1 (MQ=255)                                                                          aaaaGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCAc                                                                                                                                                                    >  2:99476/1‑251 (MQ=255)                                                                          aaaaGTGGCAATGACCGGCATTACCGCGGCTACGATATTCTTGATCGGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCAc                                                                                                                                                                    <  2:275573/251‑1 (MQ=255)                                                                             aGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCt                                                                                                                                                                 <  2:89438/251‑1 (MQ=255)                                                                              gTGGCAATGACCTGCATTACCGCGGCTACGATATACTTGATCTGGCGAACCATTGCGAATTTGAACAAGAAGCGCATCTGCAGATACACGCCCCACTGCCGCCCCGAGAAGAACTCGCAGCTTACACAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGCTGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTg                                                                                                                                                                >  1:159817/1‑251 (MQ=255)                                                                                  cAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAg                                                                                                                                                            <  2:95070/251‑1 (MQ=255)                                                                                             aTTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAgggg                                                                                                                                                 >  1:101879/1‑251 (MQ=255)                                                                                               tACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGc                                                                                                                                                <  2:74087/250‑1 (MQ=255)                                                                                                aCCGGGGCTACGAGATTCTTGATCTGGCGAAACATTGCGAATGTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGGATACCGTGCTGGAAGCCTTACCGGCGGCGGCGCACGCGATGGAGGTTATGCGCAGCGGTGTTGCCGCGCTGGGCTGCACGCTGCCAGAAAAAGAGGGGCAt                                                                                                                                              <  1:340149/251‑1 (MQ=255)                                                                                                  cgcgGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGGTTCcg                                                                                                                                                                                 <  1:301906/214‑1 (MQ=255)                                                                                                  cgcgCCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCcg                                                                                                                                                                                 >  2:301906/1‑214 (MQ=255)                                                                                                     ggCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATTCGCACCGGTGTTTCCTCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCgg                                                                                                                                         >  2:10339/1‑250 (MQ=255)                                                                                                           gATATTCTTGATCTGGCGAAACATGGCGCATTTGAAGAAGTGGCTCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTAGGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTgg                                                                                                                                   <  2:242531/251‑1 (MQ=255)                                                                                                              atTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGgcg                                                                                                                                 <  2:269991/250‑1 (MQ=255)                                                                                                                 cTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGCTCCACGGCAAACTGCCGACCAGAGACGAACTCGCCGCTTCCAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCCTAACGTGCTGGAAACCTTACCGGCGGCGTCGCACCCGCTGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCAGCACGCTGCCCAAAAACGAGGGGCATACCCTCTCTGgcgcccg                                                                                                                             >  1:129102/1‑248 (MQ=255)                                                                                                                         ggCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCTCCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGa                                                                                                                          <  1:237149/246‑1 (MQ=255)                                                                                                                         ggCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGAATATTg                                                                                                                     <  1:99476/251‑1 (MQ=255)                                                                                                                          gCGAAACATTGAGAATTCGAAGAAGCGGCGCATCTGCTGATCCCCGGCAAACTGCCCACCCGTGACGCACTCGCCGCTTACAAAACGAACCTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATCCCGTGCTGGCAGACTTACCGTCGGCCTCTCCCCCGATGGCTGGTATGCGACCCGGGTTTTCCGCGCTCCGCTGCACGCCGCCAGACAAAGGGGGGCATACCGTCGCTGGCGCGTGGGGTATTGc                                                                                                                    >  2:171302/1‑251 (MQ=255)                                                                                                                                     cGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGTAAGCCTTACCGGCGGCGTCGCACCCGATCGATGTTATGCGCACCGGTGTTTCCGCGCTCCGCTGCACGCGACCCGACAAAGAGGGGCATACCGTCTCTGTCGCGCGGGATAGTCCCGACaaccggc                                                                                                         >  2:5378/1‑246 (MQ=255)                                                                                                                                       aaTTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAAgtgctg                                                                                                       <  1:80022/251‑1 (MQ=255)                                                                                                                                            gaagaaGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTc                                                                                                  >  1:110462/1‑251 (MQ=255)                                                                                                                                               gaagTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGAGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCt                                                                                               >  1:23820/1‑251 (MQ=255)                                                                                                                                                aagTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCtt                                                                                              <  1:332016/251‑1 (MQ=255)                                                                                                                                                             gctgATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACAGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTcc                                                                                 >  1:306093/1‑251 (MQ=255)                                                                                                                                                               tgATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCtt                                                                               >  1:205848/1‑251 (MQ=255)                                                                                                                                                                   ccACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAtt                                                                           >  2:261161/1‑251 (MQ=255)                                                                                                                                                                          aaaCTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGGGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCa                                                                    <  2:171615/251‑1 (MQ=255)                                                                                                                                                                                ccGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCcgcg                                                                                                                                                                               >  1:166392/1‑138 (MQ=255)                                                                                                                                                                                ccGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCcgcg                                                                                                                                                                               <  2:166392/138‑1 (MQ=255)                                                                                                                                                                                ccGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGGGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCGGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAg                                                              <  2:51314/251‑1 (MQ=255)                                                                                                                                                                                 cGACCCGGGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCGCCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGc                                                             <  2:267617/251‑1 (MQ=255)                                                                                                                                                                                                    cgcTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATcc                                          >  1:225856/1‑251 (MQ=255)                                                                                                                                                                                                        taaaaaaCGAAACGTAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCaa                                        <  2:110462/246‑1 (MQ=255)                                                                                                                                                                                                         aCAAAACGAAACTGAAAGCCCGGCGCGGTTTACCGGCTAACGTGCATGCCGTGCTGGAAGCCTTACCGGCGGCGTCGCACGCGATGGATGTTATGCGCGCCGGTGTTTCCGCGCTCCGCTGCACGCTGCCAGAAAAAGAGGGTCATGCCGTCTCGGGCGCTCGGTATATTGCCGGCAAACTGCTGGCGTCGCTTAGGTCGATTCTCCTGTAGGGGGATCACTACAGCCACAACGGCGAACGCATCCAAcc                                      <  1:46828/250‑1 (MQ=255)                                                                                                                                                                                                          cAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCgg                                    >  1:234766/1‑251 (MQ=255)                                                                                                                                                                                                           aaaaCGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTCTTGGTATCACTACAGCCACAACGGCGAACGCATCCCACCggg                                   >  1:3906/1‑250 (MQ=255)                                                                                                                                                                                                                                   ggTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATcggcgg           >  1:35280/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggTTTACAGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCCCCAGATGGATGTTATGCGCACCGGTGGTTCCGCGCTCGGATGCACGCTGCCAGAAAAAGACGGGCATACCGTCTCTGGAGCGCGTGATATTGCCGACAAACTACTGCCGTCGCTTAGCTCGATTTTCCTTTATTGGTATTACTACATCCACAACGGCGGACGCCGCCAGCCGGCAACCGTAGACGACCCCaaagaagg           >  2:198893/1‑244 (MQ=255)                                                                                                                                                                                                                                          cGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTc    <  1:345991/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTc    <  2:234766/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATcggcgggtaactcc   >  2:156160/1‑243 (MQ=255)                                                                                                                                                                                                                                           ggCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTcc   <  1:168367/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCTAACGTGCATACCGTGCTGGAGGCCTTACCGGCTGCGTCGCACCCGATGGATGTGATGCTCACCGGTGTTTCCGCGCTCGGCTGCACGCTTCCAGAAAAAGAGGGGCATGCCGTCTCTGGCGCTCGGGAGTTTTCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTGTATTGGTATCACTACTGCCACATGGCCGAACGCATCCAACCGGAAACCGATgacga                       <  1:159881/230‑1 (MQ=255)                                                                                                                                                                                                                                            gCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGCTGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGACAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACCAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATgacgaagatcggaagaagtcgtgt    >  2:159881/1‑230 (MQ=255)                                                                                                                                                                                                                                            gCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATTTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCTCGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGTGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCt  <  1:50964/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCCGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCCCTTccc  >  1:50724/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCT  >  NC_000913/350047‑350532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑