Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,039,555 A→G L26L (TTA→TTG)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,039,5550AG94.7% 52.9 / ‑5.1 19L26L (TTA→TTG) msrPprotein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/3);  minor base T (0/1);  total (15/4)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GCCTTTATTACTTAGCCAATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTA  >  NC_000913/2039321‑2039784
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     
gCCTTTATTACTTAGCCAATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTgg                                                                                                                                                                                                                             >  1:29567/1‑245 (MQ=255)
gCCTTTATTACTTAGCCAATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTgg                                                                                                                                                                                                                             <  2:29567/245‑1 (MQ=255)
      attaCTAAGCCAATAGTGGTATTCAACCGATAGTGTCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTATTGGTAGGGATGTCTCTCCCTGTCAGATGAACAGTAAACGGTTCTTGCTGGCTAACGACAAAAAAGTGTGATGTCTGATGAAGAGGAATCAATTTTGAAAAGAAGCTGATGTGCCGGCGGAGTCAGTAAGCTAGGTGAGGCGTCGGGAGGTGTGTAAGGCACTGGCCATAAGTGCa                                                                                                                                                                                                                  <  2:227202/250‑1 (MQ=255)
                 aaTATGGGTATTCCACCTATAGTAGGAGCTAATGCATCACTCTGTAAACGCAGATCTCTACTCATGTCAAGGCGGACACTCGCTGACAGCTTAACAGTAAACGACTCTTGCTGGCTAACGACAAAAAAGTGAGATGGCTTATGAAAACGAATCAATTTTTAAAAGAATCAGATGTTACGGCAGAGTCGGAATTCTTTATGAAGCGTCGGCAGGGGTTGAAAGGACAGGGAATCAGTGCacatgcacgtttt                                                                                                                                                                                                      >  2:363590/1‑239 (MQ=255)
                                               aaTGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCtt                                                                                                                                                                        >  1:197594/1‑251 (MQ=255)
                                                                   cAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAACCGGCTCTTGCTGGCTCACGACAACAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTAGGTCTTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTACAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTaaatggcacg                                                                                                                                                     >  2:237746/1‑243 (MQ=255)
                                                                            gCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgccc                                                                                                                                              <  2:197594/248‑1 (MQ=255)
                                                                                           gaCACTCCCTGCCAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGttt                                                                                                                                                               >  1:186137/1‑216 (MQ=255)
                                                                                           gaCACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGAGGTCTTATGAAAAAGAATCAATGTTTAAAAGAATCAGATGTTACGGCCTAGTCGGTATTCTTTATGAAGCGTTGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGtgt                                                                                                                                                               <  2:186137/216‑3 (MQ=255)
                                                                                                    tGACAGATTAACAGTACACGGCTCTTGCTGCCTAACGACAAGAAAGTGAGATGGCATATGAAAACCAATAAATTTTTAAAAGAATCAGATGATACGGACGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGAACTTTCTTTGCCTCAAGCTGAGAATGCCAATCTGCTTGGCTGGTTTAAAGGGAAAGATCGCCCGCCCGCCCCCGCCGGAACAGCGCTggg                                                                                                                   >  2:361741/1‑250 (MQ=255)
                                                                                                     gACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGACCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGAGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTgggg                                                                                                                  >  1:184668/1‑249 (MQ=255)
                                                                                                                          tctTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAACAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGGATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTTAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTGCAGCAAGCCTGCCGCCTgg                                                                                             >  2:22235/1‑251 (MQ=255)
                                                                                                                                 ggCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAAt                                                                                       >  1:8974/1‑250 (MQ=255)
                                                                                                                                       cGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGc                                                                                >  1:22211/1‑251 (MQ=255)
                                                                                                                                                                aaaaaGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTAGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGATGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCC‑GCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc                                                      >  2:132620/1‑251 (MQ=255)
                                                                                                                                                                              tttAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGGGTTGAAAGCACTGGGCATCAGGGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGGTATAATAACt                                         >  2:69384/1‑251 (MQ=255)
                                                                                                                                                                                     gAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATg                                   >  1:59718/1‑250 (MQ=255)
                                                                                                                                                                                                        aGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGcc               >  1:128492/1‑251 (MQ=255)
                                                                                                                                                                                                                      aTGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCCAAATAACCTGCCACTGACGCCAGCCGATAAAGGTTCCGGTTATAATAACTTCTATGACTTCGGGCTGGATAAAGCCGATACCGCCGCTa  >  2:275459/1‑250 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     
GCCTTTATTACTTAGCCAATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTA  >  NC_000913/2039321‑2039784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑