Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,314 G→A S120S (TCG→TCA)  yeaH → DUF444 domain‑containing protein YeaH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,3140GA100.0% 84.7 / NA 26S120S (TCG→TCA) yeaHDUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

GACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGA  >  NC_000913/1869087‑1869559
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
ggagctgACAGCGGCGAATCCTTATCCCTGCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTTGCGGGATGCGCTACCGAGTGCATCCGGGCAATGACCATTTCGTCCAGAGCGACCGAATTGAACGTCCCCAGGGTGGCGGCGGATGTTCCGGCAGTGGTCATGGCCAGGCCATCCAGGATGGTGAAGGTCAGTATGAGTTTTTCTTTCATATTTCAAAAGATGATTATCTTGATCTGct                                                                                                                                                                                                                                <  2:150665/245‑1 (MQ=255)
gacGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTg                                                                                                                                                                                                                                  <  2:301600/249‑1 (MQ=255)
   gTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCCGTGGTCAGGGCCCGGCCCGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCtt                                                                                                                                                                                                                             >  2:266229/1‑251 (MQ=255)
        cAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAg                                                                                                                                                                                                                        <  1:266229/251‑1 (MQ=255)
         aGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGa                                                                                                                                                                                                                       <  1:174624/251‑1 (MQ=255)
             gcgAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTg                                                                                                                                                                                                                   <  1:222983/251‑1 (MQ=255)
                                                     gATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTgc                                                                                                                                                                           >  1:70297/1‑250 (MQ=255)
                                                       tGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc                                                                                                                                                                         >  1:67142/1‑251 (MQ=255)
                                                           tcatcaGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCCGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTTGCCTTAACGAATCTGAAACAAAACCAACACCGCCAGCTGACCGAat                                                                                                                                                                     >  1:121228/1‑251 (MQ=255)
                                                           tcatcaGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGaa                                                                                                                                                                      <  1:261959/250‑1 (MQ=255)
                                                                        ggCGGTCTGCGCCACCGCGTGCCTCCGGGCAATGCCAATTTCGTCCAGACAGACCGAAATAACCGTCCCCAGGGAGGCGGAGGAGGATCCGGCAGTGGACAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGCTGACTTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATcg                                                                                                                                                        >  2:215435/1‑251 (MQ=255)
                                                                              cTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGTATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACaaaa                                                                                                                                                                                            <  2:291342/209‑1 (MQ=255)
                                                                              cTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACaaaa                                                                                                                                                                                            >  1:291342/1‑209 (MQ=255)
                                                                                                                        gaCGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAAGTTCCGGAAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAACGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAACATCAGCGTTGTGCg                                                                                                        >  2:25296/2‑251 (MQ=255)
                                                                                                                              cGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCAtt                                                                                                  <  1:303079/251‑1 (MQ=255)
                                                                                                                                      aCGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGCTTTCAAAAGATGAGTATCTTGATCt                                                                                                                                                                                                                                   >  2:86457/1‑114 (MQ=255)
                                                                                                                                      aCGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCt                                                                                                                                                                                                                                   <  1:86457/114‑1 (MQ=255)
                                                                                                                                        gTCCCCAGGGTGGCGGCTGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCTAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCTTCCAATTTCAGCGTTGTGCGTTCATTGCAGAACTCa                                                                                        <  2:181976/251‑1 (MQ=255)
                                                                                                                                                      ggcggAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCTGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGcac                                                                          >  1:253704/1‑251 (MQ=255)
                                                                                                                                                        cggAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATAATGAAGGACAGGATGCATTTGTATTTCAGACTTCAAAAGATGAGTATCTTGCTCTACTCTTTGAAGATCTGGCATTACCGAATCTGAAACAAAACCAACAACGCCAGATGACCGAATATAAAAAGCATCGGACGGGTTATACCGCTAACGCAGTTACGGCCAATATCCGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGcac                                                                          >  1:355550/1‑249 (MQ=255)
                                                                                                                                                                             gggCCAGGGCCGACAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGAGCTGCTCTTTGAAGAGTTGGCCTTACCGAATCGGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACTGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGCCGCACAGCCATGACGGCAGGCTAgcggc                                                   <  2:167353/251‑1 (MQ=255)
                                                                                                                                                                                 caggccagCCAGGATGGTGAAGGTCAGGCTGAATTTGTCTTTCAGATTTCAAAAGCTGAGTATCTTGCTCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCgggg                                               >  2:31432/1‑250 (MQ=255)
                                                                                                                                                                                                 gTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGaa                               <  1:45026/251‑1 (MQ=255)
                                                                                                                                                                                                                   ttGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCcatcat              <  1:17353/250‑1 (MQ=255)
                                                                                                                                                                                                                     gTCTTTCAGATGTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAg           <  2:73133/251‑1 (MQ=255)
                                                                                                                                                                                                                               tttCAAAAGATGAGTATCTTGATCTGCTCTTTGACGATTTGGCCTTACCGAATCTGAAACAAAACGAACAACGCCAGCTGACCGATTATAAAACGCATCGGGCGGGTTATACCGCTAAAGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACAGCAGGCAAGCGGCGGGAACTTCATACACCTGAAGAGACTTTGGCCATCATCAGCAACAGTGa  >  1:144811/1‑250 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
GACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGA  >  NC_000913/1869087‑1869559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑