Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	299,490	T→C	E482G (GAA→GGA)	paoC ←	aldehyde dehydrogenase: molybdenum cofactor‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	299,490	0	T	C	98.0%	158.2 / NA	50	E482G (GAA→GGA)	paoC	aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/17);  minor base G (0/1);  total (32/18)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTTCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTCCGGTGCGCAAGCAC  >  NC_000913/299243‑299726                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              gccgccTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCg                                                                                                                                                                                                                                            >  1:243278/1‑251 (MQ=255)        gCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGcc                                                                                                                                                                                                                                       <  2:183234/249‑1 (MQ=255)               cTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGcc                                                                                                                                                                                                                              >  1:91280/1‑251 (MQ=255)                         cGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGgtgt                                                                                                                                                                                                                    >  1:277823/1‑251 (MQ=255)                            ggCGCTTCGGGTACCGTTGGTAATCTGGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAAGGGGGTAGc                                                                                                                                                                                                                 <  2:290226/251‑1 (MQ=255)                                cTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGcc                                                                                                                                                                                                             >  1:102160/1‑251 (MQ=255)                                    gggTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTg                                                                                                                                                                                                         >  1:299132/1‑251 (MQ=255)                                          cGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACCGGCGGCGTAAACGCCGGAGGTGGCGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATg                                                                                                                                                                                                   >  1:17826/1‑251 (MQ=255)                                              ggTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCg                                                                                                                                                                                                <  2:353911/250‑1 (MQ=255)                                                  aTCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTACCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCAt                                                                                                                                                                                           >  2:74867/1‑251 (MQ=255)                                                       gCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGTTATTCGCGCCCCAT‑GTGCCACCAGAACCCGCAGAAACCGGGAAACGGGAATCGCCGAGGTGAACCGCAACCTGTTCCATCGGTACTCCATTTAGTGCCGCTGCCGTCGGGGCCAGAATGGTGTAGCGGCCTTTGCCAATGTCTGTCATGTcc                                                                                                                                                                                       <  2:158471/250‑1 (MQ=255)                                                       gCCGTCGGCAAACTGCGACTGCTCAGGAGCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTGCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCATCCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCg                                                                                                                                                                                      <  2:25903/251‑1 (MQ=255)                                                          gTCGGCAAACTGCGACTGCTCAGGATCAAACCCGCCTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGttt                                                                                                                                                                                   >  1:222941/1‑251 (MQ=255)                                                          gTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCACACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGAGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGCCTGGGCCAGAATGGTGTAGCTGCCGGTGCCCATGTCGGTCATGTCCGttt                                                                                                                                                                                   >  1:20422/1‑251 (MQ=255)                                                           tCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTc                                                                                                                                                                                  >  1:34653/1‑251 (MQ=255)                                                             ggcgaaCTGCGCCTGCACAGGAGTAAACCCGGTGGCCGAGGCAATCATTTCGCGAGGCGTCATACAGGCGGCGGAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCTCCGAGGTGAACCGCAACCGGCGCCAGCGGTACGCCAATCATTCCCGCTGCCGTCTGGGCCAGAATGGGGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTa                                                                                                                                                                                <  2:11439/247‑1 (MQ=255)                                                                   cTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTa                                                                                                                                                                           >  2:215874/1‑250 (MQ=255)                                                                               aGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCACCGAGGTGAACCGCAACCTGCTCCCGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGGATGGGGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCGACGGGAACGGGGCCGtttt                                                                                                                                                              >  2:98783/1‑251 (MQ=255)                                                                                 gATCAACCCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACCGGCGGCGTAAACGCAGGAGGCGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGCAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCCTGTCCGTTTCTACGGTAACGGTGCCGTTTTGt                                                                                                                                                            >  2:167115/1‑251 (MQ=255)                                                                                      aaCCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTTGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGTAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGTTGCCAATGTCGTTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAg                                                                                                                                                       <  1:17059/251‑1 (MQ=255)                                                                                        cccGGCTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCAGTGT‑CCTCCAGAACCCTCAGAAACCGGGAAACTGGAACCGCCGAGGTGAACCGCATCCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGTCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTATCGGTGCCGTTTTGTTCGAGGt                                                                                                                                                     <  1:147867/251‑1 (MQ=255)                                                                                        cccGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGt                                                                                                                                                     >  1:358427/1‑251 (MQ=255)                                                                                         ccGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGTCGTAAACCCCGGAGGTGGAGGTATTCGCGCCCCATGGT‑CCACCAGAACCCGCATAAACCGGGAAACTGGAATCTCCGAGGTGAACCGCAGCCTGCTCCAGCGGTACGCCAAGCAGGCCCTCTTCCGTCTGGGCCAGAAGGGTGTAGCTGCCGGTGTCGATGTCGGTCATTTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTg                                                                                                                                                    <  1:343929/251‑1 (MQ=255)                                                                                           gACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGCGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGa                                                                                                                                                   >  1:179512/1‑250 (MQ=255)                                                                                                cggcggccatcaggtgtcgaggCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTTTGCGCGCCCCCTTGT‑CCAGCAGAACCCGCAGAAACCGGGAAACTGGAACCGCCGAGGTGAGCAGCAACCTGCTCCAGCGGTACGCCAAGGATTCCCGCTGCCGTCTGGTCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCAGGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGa                                                                                                                                             <  2:191607/230‑1 (MQ=255)                                                                                                               cgcgAAGCTTCAGACAGGCGGCGTAAACGCCGGAGGGGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACGGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTcc                                                                                                                              <  1:235905/251‑1 (MQ=255)                                                                                                                            aCAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTg                                                                                                                  <  1:271357/250‑1 (MQ=255)                                                                                                                              aGGCGGTGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCg                                                                                                                >  1:103426/1‑250 (MQ=255)                                                                                                                                   gcgTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGcc                                                                                                          >  1:18584/1‑251 (MQ=255)                                                                                                                                      tAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGCGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCccgc                                                                                                       >  1:54316/1‑251 (MQ=255)                                                                                                                                      tAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑ACACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCCGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCccgc                                                                                                       >  1:351314/1‑251 (MQ=255)                                                                                                                                       aaaCGCCGGAGGTGGAGGTATGAGCGCCCCATAGC‑CCACCAGGACACGCAGAAACCGGGAAACTGGAATCGCCGAGGGGAACCGCAGCCGGCTCCAGAGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCTGGTGCCAATGTCGGTCATGTCTGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCAACAGATTTTTCCAGCAGATTATTGTGACAGccctc                                                                                                       <  2:90423/250‑3 (MQ=255)                                                                                                                                                  gtggaggtATGCGCGCCCCATTGC‑CCACCAGACCCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAAGGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTg                                                                                           <  1:56590/251‑1 (MQ=255)                                                                                                                                                     gaggtATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGcc                                                                                        >  1:202166/1‑251 (MQ=255)                                                                                                                                                     gaggtATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGcc                                                                                        >  1:155020/1‑251 (MQ=255)                                                                                                                                                        gtATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGAc                                                                                     >  1:130861/1‑251 (MQ=255)                                                                                                                                                               cgcCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGCAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCAc                                                                              >  1:304751/1‑251 (MQ=255)                                                                                                                                                                       tGT‑CCACAAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTc                                                                      >  1:264378/1‑251 (MQ=255)                                                                                                                                                                        gT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCCGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTcc                                                                     >  2:303470/1‑250 (MQ=255)                                                                                                                                                                           ccaccaGAACCCGCAGAAACCGGGAACCGGGAACCGCCGAGGTGAACCGCACCCTGTTCCAGCGGTACGCCAGGCATCCCCGTTCCCGGCTGGGCCAGAATGGGTTAGCTGCGGGTGCAAATGGTGGTGATGCCCGTTTCGCCGGTATCGGTGCCGTTTTGTGGGAGGTGAACCCGAGCACCCGTTTTTTCCACCAAATTATTCCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTcgcg                                                                   <  1:244322/251‑1 (MQ=255)                                                                                                                                                                               caGAACCCGCAGAAAACGGGAAACTGGAATCGCCGCGGTGAACCGCAACCTGCTACAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGCGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCCCGC‑Acc                                                              >  2:152200/1‑251 (MQ=255)                                                                                                                                                                                    cccGCAGAACCCGGGAACCTGGAATCGCCGAGGTGACCCGCAACCTCCCCCAGCGGTACGCCAAGCATCCCCGCTGCCGTCTGGGCCAGAATGGTGTGGCTGCCGGTGCCAATGTCGGTCAGGTCCTTGTCTACGGTAACGGTGCCGTTTTGGTCGAGGTGAACCCTACCCCCCGATTTTCCCAGCAGATTATGGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTcc                                                         <  1:167115/251‑1 (MQ=255)                                                                                                                                                                                          gAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCCGCCGTCTGGGCCAGAATGGAGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGGGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCCGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGgtgt                                                   >  1:251488/1‑251 (MQ=255)                                                                                                                                                                                           aaaccgcgaacatgcgatcaccGAGGTCAACCGCCACCTCCTCCAGCGGTACGCCCAGAATTCCCGCAGACGTCTCGGCCAGAATGGAGTAACTGCCGATGCCAATGTCGGTCACATCAGTCTCTACGGTAACGGCGCCGTTTTGTTCGAGGGGACCCCGAGCACCCGACTTTTCCAGCAGATTATTGCGAAAGCCCGCCGAAACACCGCGGCCCACTAGCCCCTCCCCGCCGCGCCACCTG‑CCGGGcgtg                                                  >  2:54052/21‑251 (MQ=255)                                                                                                                                                                                                   aaaCTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTgcg                                          >  1:4687/1‑251 (MQ=255)                                                                                                                                                                                                                     tGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAAttt                        >  1:160731/1‑251 (MQ=255)                                                                                                                                                                                                                             cccctccTCCAGCGGTCCGCCAGGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTCCCATTTTCGTTCATGCCCTTTTCTACGGTAACGGTGCCTTTTTGTTCGAGGTGACCCCGAGCACCCGTTTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTc                <  1:71047/245‑1 (MQ=255)                                                                                                                                                                                                                                cTGCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTCCgg             >  2:189413/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCTCCAGCGGTACGCCAAGCATTCCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTCCGGTg           >  1:320268/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTACGCCAAGCATTCCCGCTGCCGTCGGGGCCATAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGCCCTTTTCTACGTTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCCCCCGATTTTTCCAGCATATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTCCGGTGCGCAAGCAc  <  1:114575/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              GCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACTGCGACTGCTCAGGATCAAACCCGACTGCCGAGGCAATCATTTCGCGAAGCTTCATACAGGCGGCGTAAACGCCGGAGGTGGAGGTATTCGCGCCCCATTGT‑CCACCAGAACCCGCAGAAACCGGGAAACTGGAATCGCCGAGGTGAACCGCAACCTGCTCCAGCGGTACGCCAAGCATTTCCGCTGCCGTCTGGGCCAGAATGGTGTAGCTGCCGGTGCCAATGTCGGTCATGTCCGTTTCTACGGTAACGGTGCCGTTTTGTTCGAGGTGAACCCGAGCACCCGATTTTTCCAGCAGATTATTGCGAAAGCCCGCCGCAACACCGTGGCCGACTAGCCACTCCCCGTCGCGC‑ACCTGTCCGGGTGTGGCGTTGCGCTGCTTCCAGCCAAATTTATCCGCTCCGGTGCGCAAGCAC  >  NC_000913/299243‑299726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑