| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,605,953 | G→A | Q301Q (CAG→CAA) | lsrB → | Autoinducer‑2 ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,605,953 | 0 | G | A | 95.5% | 70.0 / NA | 22 | Q301Q (CAG→CAA) | lsrB | Autoinducer‑2 ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/9); minor base C (1/0); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCT > NC_000913/1605711‑1606194 | gATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCCGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGt > 1:214969/1‑251 (MQ=255) caAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATg > 1:133339/1‑250 (MQ=255) cAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGaa < 1:105803/251‑1 (MQ=255) gTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACt > 1:319240/1‑251 (MQ=255) cGGATTCAGTACGACAAATGTGATGCGCCCGTATGTAGAGCGCGGCCCGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAgc > 2:48946/1‑251 (MQ=255) aGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTg > 2:339420/1‑234 (MQ=255) aGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTg < 1:339420/234‑1 (MQ=255) agagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCaaa < 1:21237/247‑1 (MQ=255) agagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCaaa > 2:21237/1‑247 (MQ=255) agCGCGGCAAGGGGAAAGAATTTGGACTGAGGGATGAGGTTCAGCACGGCAACATTTAAGAGAATGTAGCGGCCGCCTTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCACGTTGAAGTCTCGCCAAACACCGTTCCGGGCTATGACTACGAAGCGGATGGTACTGGCATCGTACTGTTACCGGAGCGCGTGATCTTCAACAAAGAGAATCACGGCCAATACgct > 2:162277/1‑249 (MQ=255) aCGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTAACGGAGCGCGTGATATTCAACAAAGCGAATATCGGCAAATACGATTTCTGAtg > 2:82819/1‑251 (MQ=255) gTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGc < 1:354267/251‑1 (MQ=255) tgtATGTCGCGGATGCATTATTGACAAAAGGATCAATGCAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTAATTACCCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATg > 2:242720/1‑251 (MQ=255) gTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTaaa < 2:242187/251‑1 (MQ=255) gAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAACGATGGTAAAGAATTTCGTACCGATc > 1:262531/1‑250 (MQ=255) aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGTAGCGGATGGTAATGGCATCGTACTTTTACCGGAGCGTGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGAGATTAAAGGTGGTAAAGTTTGTCGTACCGATCaa < 2:203119/251‑1 (MQ=255) aGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGCTTTCTGATGTGCCTTACTTAACCGGAGTAAGTTATGGAGGTTTTAGGCGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGc > 2:343962/1‑250 (MQ=255) aTGAAAACGTGCGTCAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGTGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTTCTTAACCGGAGTAAGTTATGGCATATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAat < 1:183469/251‑1 (MQ=255) aaaaCGGGCGACAAGCTGGATATCAAGGGCGGAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATc < 2:214969/251‑1 (MQ=255) aaGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGCAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGCGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGAAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCACTTTTACCCTg > 1:232993/1‑251 (MQ=255) ggCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCCGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGCGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTgcg > 2:333796/1‑251 (MQ=255) cGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCt < 2:319240/251‑1 (MQ=255) | GATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCT > NC_000913/1605711‑1606194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |