Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,242,845	G→A	A79V (GCC→GTC)	ldcA ←	murein tetrapeptide carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,242,845	0	G	A	96.9%	94.5 / NA	32	A79V (GCC→GTC)	ldcA	murein tetrapeptide carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/17);  minor base C (0/1);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGG  >  NC_000913/1242598‑1243082                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              cccTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGacac                                                                                                                                                                                                                                            <  2:193503/251‑1 (MQ=255)                                          gccagAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAAGGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTa                                                                                                                                                                                                   <  1:22058/251‑1 (MQ=255)                                                  tgtgacTCGGAAAAGCCGTCCAGTCCACCCGCCCCAAATTTGGCT‑CCAGGCATGGGGCCGCTAAAGTTGATGACATTGCCATGAGCCAGAGGACCGCACGGAAGGGCGGTAAAACCGCTA‑GTGCCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCATTGCCTGCCAGTCAATATCGGCCAGTAAACGACTGACCCCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTagtcagtc                                                                                                                                                                                            <  2:87620/245‑1 (MQ=255)                                                     ggCTCGGTAAAGGCGTTCAGTTCAGCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACGCCGTAACCGCCGCTTACAGCCAGCACGATTGTGTTGGGGGTAGTCAGTCTGGc                                                                                                                                                                                        <  1:305760/250‑1 (MQ=255)                                                      ccgagcgaaaGGCGTGCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACGAAATGGCGGTAAAATCGCTATTT‑CCGCAAATGAG‑CACCGGGTCATGTTGTGGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCa                                                                                                                                                                                       <  2:29900/244‑1 (MQ=255)                                                             aaaGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCCCGATGGTGTTGGGGGTAGTCAGTCTGGCa                                                                                                                                                                                       >  1:29900/1‑244 (MQ=255)                                                                                                   g‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCCTGTTGTTGGCGAGCCACCAGTGCCCGCCAGTCCATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCaaa                                                                                                                                           >  1:141063/1‑250 (MQ=255)                                                                                                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGCCCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCtt                                                                                                                                                                       <  2:192779/211‑1 (MQ=255)                                                                                                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CCACGGGGCATGTTGTTGGCGCGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACAACGTAACCGCCGCGTACAGCCAGCACGGTGGTGTTGGGGGGAGTCAGTCTGGCAAGGGACTTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCCAAACGCTCACAacg                                                                                                                               >  2:128905/1‑251 (MQ=255)                                                                                                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCtt                                                                                                                                                                       >  1:192779/1‑211 (MQ=255)                                                                                                                    ggtgagatctttcccATGGGCCAGATGACCGCACTAAAGGGCGGTAAAATGGCTATGT‑CCGCAAATGGG‑CAACGGGTCATTTGGTGGGCGAGCCACCAGTCCCTGCCAGACAATGTCTGCCAGTAAACGTCTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGGCGATCTGTTTCCGTTCCCGCAAAACGCTCACAACGACggg                                                                                                                          <  2:2277/238‑1 (MQ=255)                                                                                                                          gaCATTGCCAGGCGCCAGAAGACCGCACTGAAGGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGGCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGATAGCTGCCAGACGCTCTGTTGCCGTACCCGCAAAACGCTCACAACGACGGGCAATaa                                                                                                                    <  1:135773/251‑1 (MQ=255)                                                                                                                            cATTGCCAGGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTGGTTGGCGAGCCACCGGGGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAAcc                                                                                                                  <  2:111563/251‑1 (MQ=255)                                                                                                                               tGCCATGCGCCAGAAGACCGCACTGAAGGGCGGTAAAATCGCTATGT‑CCGCAAAGGAG‑CAACGGGTCAGGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATAGCTGCCAGTAAACGACTGACGCCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTc                                                                                                                <  1:128905/250‑1 (MQ=255)                                                                                                                                   aTGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGCG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGCCGGGCAATAACCTCGACGt                                                                                                           >  1:343859/1‑251 (MQ=255)                                                                                                                                    ggggcCAGAAGACCGCACTGATGGGCGGTAAAATCGCTATGT‑CCGCAA‑TGAGGCAACGGGTCATGTTGTGGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGtt                                                                                                          <  2:123513/248‑1 (MQ=255)                                                                                                                                             agcccGCACTGAAGGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTg                                                                                                 <  1:188460/248‑1 (MQ=255)                                                                                                                                                    aCTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGGTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATTcccc                                                                                          >  2:82927/1‑251 (MQ=255)                                                                                                                                                          gggCGTTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCGCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCg                                                                                    <  1:348131/250‑1 (MQ=255)                                                                                                                                                            gCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGt                                                                                  >  1:200951/1‑251 (MQ=255)                                                                                                                                                                   aaTCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGgcgc                                                                           >  1:311926/1‑251 (MQ=255)                                                                                                                                                                     atcgcgatgcccggaagagAG‑CACCGGGTCAGGTTGTGGGGTAGCCCCCAGGGCCTGCCAGTCAATATCTGCAAGTAAAAGACGGACCCCGTACCCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCCGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGCCGGTAAGGCGCt                                                                          <  2:111527/233‑1 (MQ=255)                                                                                                                                                                       gCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTgg                                                                        >  2:231172/1‑250 (MQ=255)                                                                                                                                                                                  aaaaTGAG‑AAAGGGGTCATGTGTTTGGCGAGCCACCAGTGCCTGCCAGTCAATAGCTGCCAGTAAACGACGGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCACCGCGTCGGTAAGGCGCTGGATACCACGCAg                                                             <  2:114677/250‑1 (MQ=255)                                                                                                                                                                                         g‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCgcggcg                                                      >  2:302006/1‑251 (MQ=255)                                                                                                                                                                                            aaCGGGTCATGTTGTTGGCAAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGCCTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGCCTTGATGCCCCGCGTCGGTAAGGCGCTGGCTACAACGCAGCGCGGCGTg                                                    >  2:352568/1‑251 (MQ=255)                                                                                                                                                                                                ggTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGCGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGGAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGt                                                >  2:72629/1‑251 (MQ=255)                                                                                                                                                                                                        tgttgGCTAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGc                                         >  1:199038/1‑250 (MQ=255)                                                                                                                                                                                                               gagccccccgttcctgccAGCCAATATCTGCCAGTAAACGACGTACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAAccc                                 <  2:272941/239‑1 (MQ=255)                                                                                                                                                                                                                               ccAGTTAATATCTGCCAGTAAGCGACTGACACCTTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAAGCTGGCAAGGGTATGGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCTGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGt                 <  2:255043/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTCAATATCTGCCAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGAGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGa               >  1:279129/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccaatagcggcatgTAAACTACTGCCGCCGTACCCGCCGCGGACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGTATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAGCCGCGACGTTATTGGCTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCGCGCAGCGCGGCGTGCGGTTTAATTCAGTAACCCTACGTGGCAATTAAGTGaaa             <  2:363904/238‑1 (MQ=255)                                                                                                                                                                                                                                              cAGTAAACGACTGACACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATgg  <  1:72499/251‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              CCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAG‑CATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGT‑CCGCAAATGAG‑CAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGG  >  NC_000913/1242598‑1243082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑