Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,847	G→A	Q121Q (CAG→CAA)	matP →	macrodomain Ter protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,847	0	G	A	100.0%	43.0 / NA	14	Q121Q (CAG→CAA)	matP	macrodomain Ter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

CCGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCT  >  NC_000913/1018603‑1019064                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          ccGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGAtt                                                                                                                                                                                                                     <  1:195572/251‑1 (MQ=255)        tgtgTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATg                                                                                                                                                                                                              >  1:358659/1‑251 (MQ=255)                                    gTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAACAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATa                                                                                                                                                                                   >  2:126069/1‑250 (MQ=255)                                     ggCTGGTAAATGGGTGGATTGACAAGCATATGAAACCGGAACTGGTCAATCGGCTGAAGCAAACCAATCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATaaa                                                                                                                                                                                 <  2:340810/250‑1 (MQ=255)                                                           cAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGtctt                                                                                                                                                                          <  1:299135/236‑1 (MQ=255)                                                           cAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGtctt                                                                                                                                                                          >  2:299135/1‑236 (MQ=255)                                                               cATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGc                                                                                                                                                        >  1:252926/1‑250 (MQ=255)                                                                          ggAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTgg                                                                                                                                            <  1:172380/251‑1 (MQ=255)                                                                                                                      aGCGCCATTTTAATGCGGAACCCCAGCATACGTGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGTTCTACAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCa                                                                                                 >  2:255211/1‑250 (MQ=255)                                                                                                                      aGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCTATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTTTTGCGCAGCGTCTCGGTAAAACGCTTTCTGAAACGAGTGTTCAACTGTTTGAAGATGCGGAAAACAAAGAGAAATTCGCTAAGAAAATGTCTTCTTTGAAGCAGGATCTGCATGCATTGCTGGGTAAGGAATAACTGACGAAAGTCATGTCATTTTACTAAATGCaa                                                                                                <  2:325969/251‑1 (MQ=255)                                                                                                                                                                cgatcgatCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAAc                                                      <  1:97628/251‑1 (MQ=255)                                                                                                                                                                    cgagcTGGAATTTATTGTCTGGCAACGGCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCGTCTCTGAAGCAGGATCTGCAGTCATTGCTGGGTAATGAATAACTGACGAAAGTTAGTTCAATTTACTAAATGCAAAAAAAACCCCGCAGCATCGGTGTGGTTCGACCAGACGAGAACTTaa                                                  <  2:234259/247‑1 (MQ=255)                                                                                                                                                                                                      ggTCGTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAATTCAGTACAATTGACTAAAGGCAAAAAAA‑CCCCTCAGCAGCGTGGGTTTTCTACCAGACGAGAACTTAAGCCGGCTGCTAAGTTACAACGTCTTTGATACCttt               <  2:41835/251‑1 (MQ=255)                                                                                                                                                                                                                     cgaggTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCAGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAATTTCGATctct  >  2:121680/4‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          CCGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCT  >  NC_000913/1018603‑1019064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑