| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 928,622 | G→A | L192L (CTG→TTG) | cydC ← | glutathione/L‑cysteine ABC exporter subunit CydC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 928,622 | 0 | G | A | 86.2% | 61.3 / 5.0 | 29 | L192L (CTG→TTG) | cydC | glutathione/L‑cysteine ABC exporter subunit CydC |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (13/12); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01 | |||||||||||
AACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCG > NC_000913/928386‑928861 | aaCGCCGCCAGACGCCATCCACAGAATCAGGATCACCGCTAACGCGACAATGAGCAGCATTATCGCTTACGACAATGCGGACAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGACTTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGATGTTGGCGATACTGTCCGCGAAGATGAGGCAAATTTTGCCCGGTGc > 2:213965/1‑251 (MQ=255) gCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGc > 1:74120/1‑251 (MQ=255) cAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTg < 1:168942/251‑1 (MQ=255) aGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTgg > 1:119365/1‑251 (MQ=255) gATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGCTACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCCCGATAAAACAGCGGTGGc > 2:202890/1‑250 (MQ=255) aTCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCAt > 2:18483/1‑251 (MQ=255) aCCGCCAACGCGCCAATTAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCGCAGCTTGCCCTTGCAGCCATGCCGTCAGTTGTTGGCGATACTGCCCGCGAAGATGAGTCAAATGTTGCCCGGTGCTATTTCCCGCACGATAAAGCAGCGGTGGCATCAg < 2:176040/251‑1 (MQ=255) gCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACACTGCGGTCAGTTCAGATTGCCGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGa > 1:174198/1‑250 (MQ=255) aaTGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGAGGCATCAGGAAAAGCGTCAGt > 1:106000/1‑250 (MQ=255) gAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACa < 2:24899/251‑1 (MQ=255) aTGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGGGGCATCAGGAAAAGAGTCAGTAACATAATGCCGCCCAGCGTAAAGGCg > 2:84907/1‑251 (MQ=255) cGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTCGTTGCGTGCGATAACGATCGCTGGCACAAACAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAgg > 1:35008/1‑251 (MQ=255) gCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAAGGCCGCCTAGCGTAAAGGCTAGGTTGAAATCAAGGAAACTTAACCCGATTGTcacc < 1:119798/251‑1 (MQ=255) tCTGTAATCTCTAGTTTCGTGCGATACCGAACGCGGGCACCAAAAATGGCCAGCTCAGCTGGCCATTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGGCAAATTTTGCCCGGTGCGTTGTCCCGCACGATAAAACAGCGGGTGCATCAGGAAAAGCGTCATGAACATAATGCCGCCTAGCGTAGATGCGTGGGGGAAATCAAGTAACCTTAACTCGATTTTTACCACCAGAATca < 2:50281/250‑1 (MQ=255) gTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATcaccac > 1:70666/1‑251 (MQ=255) gcgtgcgATAACGATCGCTGGCACCAAAAATGTTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGTCGATACTGTCCTCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCTGTATCATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAATCGCccac < 1:213965/250‑1 (MQ=255) aaCGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCga < 2:119365/250‑1 (MQ=255) cGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTGGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAAc < 1:7565/250‑1 (MQ=255) cagctTGCCCGTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCTCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCg < 1:321319/249‑1 (MQ=255) tGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGAAAAGAAGATCGAGCGCATCAAc > 1:133258/1‑251 (MQ=255) gCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAAc > 2:6568/1‑250 (MQ=255) gCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAAc > 2:70387/1‑250 (MQ=255) tGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCTTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCg < 1:220593/250‑1 (MQ=255) gggCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCAcg < 2:174488/250‑1 (MQ=255) aGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCAcg < 1:18483/251‑1 (MQ=255) gTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTTAAGATGATCGAGCGTATCAACATCCGCCACCACGCGAtt > 2:73820/1‑251 (MQ=255) gATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTTAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGccc < 1:73820/251‑1 (MQ=255) gATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTCCTTTTTCCCGCCCGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGCAACATAATGCCGCCCAGCGTAAAGGCGAGAGTGAAATCAAGGACACTTAACCCGATTGTCACCGCCATACTCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGccc > 2:149671/1‑251 (MQ=255) aaGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCTCCACGCGATTGAGCAATTCGCCCTGACGATAgcgcg < 1:198623/251‑1 (MQ=255) | AACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCG > NC_000913/928386‑928861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |