Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,369	G→A	A261V (GCG→GTG)	hcr ←	NADH oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,369	0	G	A	96.7%	92.1 / NA	30	A261V (GCG→GTG)	hcr	NADH oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/9);  minor base C (1/0);  total (21/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCATATACGGAGCCGGGCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACC  >  NC_000913/911122‑911613                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gaAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCt                                                                                                                                                                                                                                                   >  1:158764/1‑251 (MQ=255)   aaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTcc                                                                                                                                                                                                                                                 >  1:267565/1‑251 (MQ=255)      gaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTCTTGCTTTCCAGCACCTCCAGt                                                                                                                                                                                                                                              >  2:193280/1‑251 (MQ=255)        ggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAg                                                                                                                                                                                                                                            <  1:207063/251‑1 (MQ=255)               aCGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGCACCAAATCCCCCTGCGGATGGCAGGAGCAGGACAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGACACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGCACGCTATTGCTTTCCAGCACCTCCAGTAGCgcggcg                                                                                                                                                                                                                                     >  2:326313/1‑246 (MQ=255)                   ttAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCa                                                                                                                                                                                                                                  >  1:206340/1‑250 (MQ=255)                     aaaTAGCCCGATGAAAGGAATATCATCGGGCATAATGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTAAGCGATTTCGGCGCCGGTCAGCGTCATTGAGCTGCTCACCGTATATTCCCCGGAAACCACTTTTGTATTACAGCAGCCGCAAACACCATCACGGCAGGCGGCGACAACCGGTACGTTATGTCTTGCCAGCCACTCCAGTGGCGGAGTGCCaccc                                                                                                                                                                                                                               >  2:186057/1‑248 (MQ=255)                       aTAGCCCGATGAAAGGAATCTCATCGGGCATAAGGCGATTATGCGCGAACCAAATCCCCCTGCGGATGGCAGGAGCCGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGCAGCGTGGTGCCAACCgg                                                                                                                                                                                                                             >  2:22011/1‑251 (MQ=255)                                                               aTGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCCGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTc                                                                                                                                                                                     >  2:131843/1‑251 (MQ=255)                                                                   gagaACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGAc                                                                                                                                                                                 >  2:117388/1‑251 (MQ=255)                                                                     gaACCAAATCCCCCTGCGGATGGCCGGAGCAGGCCAGTACGTACCCTTCATCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTGGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCATCACCTCCAGTATCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCg                                                                                                                                                                               <  2:1525/251‑1 (MQ=255)                                                                                                           aCGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTtct                                                                                                                                          <  2:267565/250‑1 (MQ=255)                                                                                                                         gATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAATAAAcgcg                                                                                                                           <  1:280644/251‑1 (MQ=255)                                                                                                                             tCGGCGTCGGGCAGCGTCATTGTGCTGCTCACCGGATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTGTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCa                                                                                                                        <  2:49330/250‑1 (MQ=255)                                                                                                                                        cAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCtt                                                                                                            <  1:302616/251‑1 (MQ=255)                                                                                                                                                 tgtgCTGATCACGGTATATTCACCGGAACCCCCTTTTGTCTTACAGCAGCCGCAAACAACCGCACGGCAGGCAGCGACAACCGGAACGATATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCt                                                                                                   >  2:39923/1‑251 (MQ=255)                                                                                                                                                  gtgCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCt                                                                                                   <  1:6808/250‑1 (MQ=255)                                                                                                                                                   tgctgcTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCACACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGc                                                                                                 >  2:208646/1‑251 (MQ=255)                                                                                                                                                            ccGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCa                                                                                         >  1:266278/1‑250 (MQ=255)                                                                                                                                                             cGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGCGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAAt                                                                                       >  1:4897/1‑251 (MQ=255)                                                                                                                                                              gTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATc                                                                                      <  1:193280/251‑1 (MQ=255)                                                                                                                                                                atatTCACCGGCAAACACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCGGGCAGCGACAACCGGAACGTTATTGATTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGATTGGTGAATTTCAGACCACTGGTCGCCGCTTCTGCTACTGGGGGGAAGAATTGCGCTTTAAAGAAACGCGGCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATccc                                                                                    >  2:329513/1‑250 (MQ=255)                                                                                                                                                                  atTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCat                                                                                   >  2:248937/1‑250 (MQ=255)                                                                                                                                                                  atTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGGGAAGAATTTCTCTTTAAAGAAACGAGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCat                                                                                   >  1:181572/1‑250 (MQ=255)                                                                                                                                                                   ttCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCatat                                                                                 >  1:127663/1‑251 (MQ=255)                                                                                                                                                                           aaaCCACTTTTGTCTTACAGCAGCCGCAAACCCCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCATATACGGAGcc                                                                         <  1:283991/251‑1 (MQ=255)                                                                                                                                                                                   tttGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCACTCCATATACGGCGCCGGGCCGc                                                                  >  2:232619/1‑250 (MQ=255)                                                                                                                                                                                                                   gcaggcacCGACAACCCGAACGTTCTTGCTTTCCAGCACCTCCAGTCAAGGGGTACCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCCGTATGGTGAATTTCAAAACGCTGGTCGCCGCTTCTGCTACTGGGGAGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCACTCCATATACGGAGCCGGGCCGCAGGTCATCACGGTACGTGAAGCGAAGTCAGGt                                  >  2:40019/1‑250 (MQ=255)                                                                                                                                                                                                                                    gAACGGTATTGCTTTCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCATATACGGATCCGGGCCGCAGGTCATCACGGTACGTGAAACTAAGTCAGGTACACCTGCCAGCAGTTcc                >  2:141391/1‑250 (MQ=255)                                                                                                                                                                                                                                                  tCCAGCACCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGAAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTAACTTCCTGCTCTACCCAATCCATATACGGAGCCGGGCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGAcc  >  2:295741/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTTCAGACCGCTGGTCGCCGCTTCTGCTACTGGGGTGAAGAATTTCTCTTTAAAGAAACGCGTCACGCCGAGCGCTTTCACTTCCTGCTCTACCCAATCCATATACGGAGCCGGGCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACC  >  NC_000913/911122‑911613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑