| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 782,011 | C→T | intergenic (+359/‑74) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 782,011 | 0 | C | T | 83.3% | 24.5 / ‑1.7 | 12 | intergenic (+359/‑74) | lysQ/nadA | tRNA‑Lys/quinolinate synthase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (5/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCGAAAT > NC_000913/781767‑782245 | cccGAATGGCGAGAGAAGCGAGTCAGCCTGCACGACCCACCAATGGAAAAAAGCGCCCTGAAGGCGCTTTTTTGCTATTCAGGGATCCTCAATTTCACTGTGTAAACCTGATGACAGCGTCAGAGCTTAATGTGCAAGCAACTCTATGTCGGTGGAATTAGGCTTAAAATGGCGCATCCTGCACATTAGGCGTAATGCGAGTGATGTTGCCCCACCATTGTACTATCTGGTATAGCATATAAAACAAATTa < 2:51478/251‑1 (MQ=255) cATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACg < 1:61512/251‑1 (MQ=255) cATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACg > 1:71303/1‑251 (MQ=255) ccTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCt < 1:43606/250‑1 (MQ=255) aCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTGTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAATAAATGACACCGATAACAGCGAATATTACGCTAATGTCGGt < 2:234557/251‑1 (MQ=255) gTCAGAGCTTACTGTGCAAGCAACTCTATGCCGGTGGAATTAGGCGAAAAACGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGa > 2:48925/1‑249 (MQ=255) aGTGACTTTTCCCCACCATTCGACTATCTTGATTAGAATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGAAGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATg > 1:222179/1‑251 (MQ=255) cccACCATTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCact > 1:159300/1‑250 (MQ=255) aTTCGACTATCTTGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATAc < 1:61136/251‑1 (MQ=255) gACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGCAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGAt > 1:12173/1‑251 (MQ=255) tGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCg < 2:159300/247‑1 (MQ=255) tGTTTAGCATATAAAATAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAAAGTTAAGCCTGTAAAACGTGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGAGGTGCTGGTTGCCCACTACTATACCGATCCCGAAAt > 2:53909/1‑251 (MQ=255) | CCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGTTTTAACGTTAAGCCTGTAAAACGAGATGGTAAGATGAGCGTAATGTTTGATCCAGACACGGCGATTTATCCTTTCCCCCCGAAGCCGACGCCGTTAAGCATTGATGAAAAAGCGTATTACCGCGAGAAGATAAAACGTCTGCTAAAAGAACGTAATGCGGTGATGGTTGCCCACTACTATACCGATCCCGAAAT > NC_000913/781767‑782245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |