Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 584,506 C→T intergenic (+76/+174) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913584,5060CT93.8% 47.0 / ‑6.9 16intergenic (+76/+174)appY/ompTDNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (11/4);  minor base A (1/0);  total (12/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCAGCAGTAGTGATGAAGTTATAGTTTTCTATACCTGCT  >  NC_000913/584281‑584737
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       
tATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGTCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTg                                                                                                                                                                                                                  <  1:233167/249‑1 (MQ=255)
         aaTGTCGTAGCCCAGACATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTAtttt                                                                                                                                                                                                       >  2:19024/1‑251 (MQ=255)
                                                              aTTTAAAGATTATTATGGCGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACAATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGTTTTAGTTAATAACCTTCTggg                                                                                                                                                  >  2:87921/1‑251 (MQ=255)
                                                                         attatGGTGACACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTagg                                                                                                                                       >  2:79533/1‑249 (MQ=255)
                                                                                 gtCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAAtt                                                                                                                               >  2:58051/1‑251 (MQ=255)
                                                                                                                  ggCGTCACAGATGGACTAA‑CCAAACACACCGACTGCTCATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATAAAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTCTGTGATTTAGTTAATACCCTTCTGGGTTTATTTTATGGGTTACTTGTTACATTGAAATGGCTAGTTATTCTCCgggtg                                                                                              >  2:174790/1‑249 (MQ=255)
                                                                                                                       cacaGATGGCACAAACAAAACA‑ATTGCCTGATAATGTTTATTACAAGCTGTCTACCAGTCAATTATACTATTATACAGCGTTTCTTCTCATGCGATATTCTGGACCTATTAATTTGTAATTGGATTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTATTACCCTTCTGGGTTTATTTTCAGGGTTACTTGTTACATTGCAATGGCTAGTTATTCCCCGGGGCGCttt                                                                                         >  2:318045/1‑251 (MQ=255)
                                                                                                                                        aaaca‑aTTGACTGATAATGTTTATTACACGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAAttt                                                                        >  2:180531/1‑251 (MQ=255)
                                                                                                                                                          tGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATAAAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACCTACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTATTTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGCAATTTTAGTTGGCGTTATTaat                                                       >  1:340599/1‑250 (MQ=255)
                                                                                                                                                                           cTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGtt                                                                                                                                   >  1:73953/1‑158 (MQ=255)
                                                                                                                                                                           cTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGtt                                                                                                                                   <  2:73953/158‑1 (MQ=255)
                                                                                                                                                                                 gTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCAGCagt                                 >  1:134333/1‑250 (MQ=255)
                                                                                                                                                                                         taataTTATACAGCGTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCAGCAGTAGtgatgc                         >  2:71358/1‑249 (MQ=255)
                                                                                                                                                                                                       gTTTTTTTTGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTGCTTAAAATGTGTACTTAAGACCAGCAGTAGTGATGAAGTTATAGTTTTCta          <  1:180747/251‑1 (MQ=255)
                                                                                                                                                                                                              ttGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATTGTTTTCTATGTGAGTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCTGGGCGATTTTCACCTCGGGGAAAGTGTAGTTGGCGGTCTTAAAATGTGTACTTAAGACCAGCAGTAGTGATGAAGTTATAGTTTTCTATACCTg    <  2:29335/250‑1 (MQ=255)
                                                                                                                                                                                                               tGATGTGATATTCTGGAACTATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTACCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTTCTTAACATGTGTACTTAAGACCAGCAGTAGTGATGAAGTTATAGTTTTCTATACCTGCt  >  2:198069/1‑251 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       
TATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACAGATGGAATAAACAAAACA‑ATTGACTGATAATGTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTTTTCTATGTGATTTAGTTAATAACCTTCTGGGTTTATTTTAAGGGTTAATTGTTACATTGAAATGGCTAGTTATTCCCCGGGGCGATTTTCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCAGCAGTAGTGATGAAGTTATAGTTTTCTATACCTGCT  >  NC_000913/584281‑584737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑